We study formation and fluorescent properties of silver nanoclusters encapsulated in condensed DNA nanoparticles. Fluorescent globular DNA nanoparticles were formed using dsDNA-clusters complex and polyallylamine as condensing agent. Fluorescence emission spectrum of single DNA nanoparticles was obtained using tip enhanced fluorescence microscopy. Fluorescent clusters in condensed DNA nanoparticles appear to be more protected against destructive damage in solution compared with the clusters synthesized on linear polymer chain. Fluorescent clusters on both dsDNA and ssDNA exhibit the same emission bands at 590 nm and 680 nm and formation efficiency suggesting likely the same binding sites. Using the density functional theory, we show that the clusters may bind to the Watson−Crick guanine−cytosine base pairs and to single DNA bases with about the same affinity.
|Журнал||Chemphyschem : a European journal of chemical physics and physical chemistry|
|Состояние||Опубликовано - 2013|