Expression of the Drosophila melanogaster limk1 gene 3'-UTRs mRNA in Yeast Saccharomyces cerevisiae

A. M. Rumyantsev, G. A. Zakharov, A. V. Zhuravlev, M. V. Padkina, E. V. Savvateeva-Popova, E. V. Sambuk

Результат исследований: Научные публикации в периодических изданияхстатья

Выдержка

The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3'-untranscribed regions (3'-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3'-UTRs' and RNA-binding proteins' interaction in post-transcriptional regulation. For this, the post-transcriptional regulation of Drosophila limk1 gene encoding for the key enzyme of actin remodeling was studied in yeast. Analysis of limkl mRNA 3'-UTRs revealed the potential sites of yeast transcriptional termination. Computer remodeling demonstrated the possibility of secondary structure formation in limkl mRNA 3'-UTRs. For an evaluation of the functional activity of Drosophila 3'-UTRs in yeast, the reporter gene PHO5 encoding for yeast acid phosphatase (AP) fused to different variants of Drosophila limk1 mRNA 3'-UTRs (513, 1075, 1554 bp) was used. Assessments of AP activity and RT-PCR demonstrated that Drosophila limkl gene 3'-UTRs were functionally active and recognized in yeast. Therefore, yeast might be used as an appropriate model system for studies of 3'-UTR's role in post-transcriptional regulation.

Язык оригиналаанглийский
Страницы (с-по)652-659
Число страниц8
ЖурналGenetika
Том50
Номер выпуска6
СостояниеОпубликовано - 1 июн 2014

Отпечаток

Drosophila melanogaster
Saccharomyces cerevisiae
Yeasts
Messenger RNA
Drosophila
Genes
RNA-Binding Proteins
Acid Phosphatase
MicroRNAs
Protein Biosynthesis
3' Untranslated Regions
Eukaryota
Reporter Genes
Actins
Polymerase Chain Reaction
Enzymes

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Rumyantsev, A. M. ; Zakharov, G. A. ; Zhuravlev, A. V. ; Padkina, M. V. ; Savvateeva-Popova, E. V. ; Sambuk, E. V. / Expression of the Drosophila melanogaster limk1 gene 3'-UTRs mRNA in Yeast Saccharomyces cerevisiae. В: Genetika. 2014 ; Том 50, № 6. стр. 652-659.
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abstract = "The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3'-untranscribed regions (3'-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3'-UTRs' and RNA-binding proteins' interaction in post-transcriptional regulation. For this, the post-transcriptional regulation of Drosophila limk1 gene encoding for the key enzyme of actin remodeling was studied in yeast. Analysis of limkl mRNA 3'-UTRs revealed the potential sites of yeast transcriptional termination. Computer remodeling demonstrated the possibility of secondary structure formation in limkl mRNA 3'-UTRs. For an evaluation of the functional activity of Drosophila 3'-UTRs in yeast, the reporter gene PHO5 encoding for yeast acid phosphatase (AP) fused to different variants of Drosophila limk1 mRNA 3'-UTRs (513, 1075, 1554 bp) was used. Assessments of AP activity and RT-PCR demonstrated that Drosophila limkl gene 3'-UTRs were functionally active and recognized in yeast. Therefore, yeast might be used as an appropriate model system for studies of 3'-UTR's role in post-transcriptional regulation.",
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Rumyantsev, AM, Zakharov, GA, Zhuravlev, AV, Padkina, MV, Savvateeva-Popova, EV & Sambuk, EV 2014, 'Expression of the Drosophila melanogaster limk1 gene 3'-UTRs mRNA in Yeast Saccharomyces cerevisiae', Genetika, том. 50, № 6, стр. 652-659.

Expression of the Drosophila melanogaster limk1 gene 3'-UTRs mRNA in Yeast Saccharomyces cerevisiae. / Rumyantsev, A. M.; Zakharov, G. A.; Zhuravlev, A. V.; Padkina, M. V.; Savvateeva-Popova, E. V.; Sambuk, E. V.

В: Genetika, Том 50, № 6, 01.06.2014, стр. 652-659.

Результат исследований: Научные публикации в периодических изданияхстатья

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AU - Rumyantsev, A. M.

AU - Zakharov, G. A.

AU - Zhuravlev, A. V.

AU - Padkina, M. V.

AU - Savvateeva-Popova, E. V.

AU - Sambuk, E. V.

PY - 2014/6/1

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N2 - The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3'-untranscribed regions (3'-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3'-UTRs' and RNA-binding proteins' interaction in post-transcriptional regulation. For this, the post-transcriptional regulation of Drosophila limk1 gene encoding for the key enzyme of actin remodeling was studied in yeast. Analysis of limkl mRNA 3'-UTRs revealed the potential sites of yeast transcriptional termination. Computer remodeling demonstrated the possibility of secondary structure formation in limkl mRNA 3'-UTRs. For an evaluation of the functional activity of Drosophila 3'-UTRs in yeast, the reporter gene PHO5 encoding for yeast acid phosphatase (AP) fused to different variants of Drosophila limk1 mRNA 3'-UTRs (513, 1075, 1554 bp) was used. Assessments of AP activity and RT-PCR demonstrated that Drosophila limkl gene 3'-UTRs were functionally active and recognized in yeast. Therefore, yeast might be used as an appropriate model system for studies of 3'-UTR's role in post-transcriptional regulation.

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