Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae

A.M. Rumyantsev, G.A. Zakharov, A.V. Zhuravlev, M.V. Padkina, E.V. Savvateeva-Popova, E.V. Sambuk

Результат исследований: Научные публикации в периодических изданияхстатья

1 цитирование (Scopus)

Выдержка

The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3′-untranscribed regions (3′-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3′-UTRs' and RNA-binding proteins' interaction in post-transcriptional regulation. For this, the post-transcriptional regulation of Drosophila limk1 gene encoding for the key enzyme of actin remodeling was studied in yeast. Analysis of limk1 mRNA 3′-UTRs revealed the potential sites of yeast transcriptional termination. Computer modeling demonstrated the possibility of secondary structure formation in limk1 mRNA 3′-UTRs. For an evaluation of the functional activity of Drosophila 3′-UTRs in yeast, the reporter gene PHO5 encoding for yeast acid phosphatase (AP) fused to different variants of Drosophila limk1 mRNA 3′-UTRs (513, 1075, 1554 bp) was used. Assessments of AP activity and RT-PCR demonstrated that Drosophila limk1 Gene 3′-U
Язык оригиналаанглийский
Страницы (с-по)569-576
ЖурналRussian Journal of Genetics
Том50
Номер выпуска6
DOI
СостояниеОпубликовано - 2014

Отпечаток

Drosophila melanogaster
Drosophila
Saccharomyces cerevisiae
Yeasts
Messenger RNA
RNA-Binding Proteins
Acid Phosphatase
MicroRNAs
Genes
Protein Biosynthesis
Eukaryota
Reporter Genes
Actins
Polymerase Chain Reaction
Enzymes

Цитировать

Rumyantsev, A.M. ; Zakharov, G.A. ; Zhuravlev, A.V. ; Padkina, M.V. ; Savvateeva-Popova, E.V. ; Sambuk, E.V. / Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae. В: Russian Journal of Genetics. 2014 ; Том 50, № 6. стр. 569-576.
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title = "Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae",
abstract = "The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3′-untranscribed regions (3′-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3′-UTRs' and RNA-binding proteins' interaction in post-transcriptional regulation. For this, the post-transcriptional regulation of Drosophila limk1 gene encoding for the key enzyme of actin remodeling was studied in yeast. Analysis of limk1 mRNA 3′-UTRs revealed the potential sites of yeast transcriptional termination. Computer modeling demonstrated the possibility of secondary structure formation in limk1 mRNA 3′-UTRs. For an evaluation of the functional activity of Drosophila 3′-UTRs in yeast, the reporter gene PHO5 encoding for yeast acid phosphatase (AP) fused to different variants of Drosophila limk1 mRNA 3′-UTRs (513, 1075, 1554 bp) was used. Assessments of AP activity and RT-PCR demonstrated that Drosophila limk1 Gene 3′-U",
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Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae. / Rumyantsev, A.M.; Zakharov, G.A.; Zhuravlev, A.V.; Padkina, M.V.; Savvateeva-Popova, E.V.; Sambuk, E.V.

В: Russian Journal of Genetics, Том 50, № 6, 2014, стр. 569-576.

Результат исследований: Научные публикации в периодических изданияхстатья

TY - JOUR

T1 - Expression of the Drosophila melanogaster limk1 gene 3′-UTRs mRNA in yeast Saccharomyces cerevisiae

AU - Rumyantsev, A.M.

AU - Zakharov, G.A.

AU - Zhuravlev, A.V.

AU - Padkina, M.V.

AU - Savvateeva-Popova, E.V.

AU - Sambuk, E.V.

PY - 2014

Y1 - 2014

N2 - The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3′-untranscribed regions (3′-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3′-UTRs' and RNA-binding proteins' interaction in post-transcriptional regulation. For this, the post-transcriptional regulation of Drosophila limk1 gene encoding for the key enzyme of actin remodeling was studied in yeast. Analysis of limk1 mRNA 3′-UTRs revealed the potential sites of yeast transcriptional termination. Computer modeling demonstrated the possibility of secondary structure formation in limk1 mRNA 3′-UTRs. For an evaluation of the functional activity of Drosophila 3′-UTRs in yeast, the reporter gene PHO5 encoding for yeast acid phosphatase (AP) fused to different variants of Drosophila limk1 mRNA 3′-UTRs (513, 1075, 1554 bp) was used. Assessments of AP activity and RT-PCR demonstrated that Drosophila limk1 Gene 3′-U

AB - The stability of mRNA and its translation efficacy in higher eukaryotes are influenced by the interaction of 3′-untranscribed regions (3′-UTRs) with microRNAs and RNA-binding proteins. Since Saccharomyces cerevisiae lack microRNAs, it is possible to evaluate the contribution of only 3′-UTRs' and RNA-binding proteins' interaction in post-transcriptional regulation. For this, the post-transcriptional regulation of Drosophila limk1 gene encoding for the key enzyme of actin remodeling was studied in yeast. Analysis of limk1 mRNA 3′-UTRs revealed the potential sites of yeast transcriptional termination. Computer modeling demonstrated the possibility of secondary structure formation in limk1 mRNA 3′-UTRs. For an evaluation of the functional activity of Drosophila 3′-UTRs in yeast, the reporter gene PHO5 encoding for yeast acid phosphatase (AP) fused to different variants of Drosophila limk1 mRNA 3′-UTRs (513, 1075, 1554 bp) was used. Assessments of AP activity and RT-PCR demonstrated that Drosophila limk1 Gene 3′-U

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