Expanding the Described Metabolome of the Marine Cyanobacterium Moorea producens JHB through Orthogonal Natural Products Workflows

P.D. Boudreau, E.A. Monroe, S. Mehrotra, S. Desfor, A. Korobeynikov, D.H. Sherman, T.F. Murray, L. Gerwick, P.C. Dorrestein, W.H. Gerwick

Результат исследований: Научные публикации в периодических изданияхстатья

13 Цитирования (Scopus)

Выдержка

Moorea producens JHB, a Jamaican strain of tropical filamentous marine cyanobacteria, has been extensively studied by traditional natural products techniques. These previous bioassay and structure guided isolations led to the discovery of two exciting classes of natural products, hectochlorin (1) and jamaicamides A (2) and B (3). In the current study, mass spectrometry-based 'molecular networking' was used to visualize the metabolome of Moorea producens JHB, and both guided and enhanced the isolation workflow, revealing additional metabolites in these compound classes. Further, we developed additional insight into the metabolic capabilities of this strain by genome sequencing analysis, which subsequently led to the isolation of a compound unrelated to the jamaicamide and hectochlorin families. Another approach involved stimulation of the biosynthesis of a minor jamaicamide metabolite by cultivation in modified media, and provided insights about the underlying biosynthetic machinery as well as preliminary stru
Язык оригиналаанглийский
ЖурналPLoS ONE
Том10
Номер выпуска7
DOI
СостояниеОпубликовано - 2015

Отпечаток

Moorea producens
metabolome
Metabolome
Workflow
Cyanobacteria
Metabolites
Biological Products
metabolites
Bioassay
Biosynthesis
Biological Assay
Machinery
Mass spectrometry
Mass Spectrometry
bioassays
Genes
biosynthesis
mass spectrometry
Genome
genome

Цитировать

Boudreau, P.D. ; Monroe, E.A. ; Mehrotra, S. ; Desfor, S. ; Korobeynikov, A. ; Sherman, D.H. ; Murray, T.F. ; Gerwick, L. ; Dorrestein, P.C. ; Gerwick, W.H. / Expanding the Described Metabolome of the Marine Cyanobacterium Moorea producens JHB through Orthogonal Natural Products Workflows. В: PLoS ONE. 2015 ; Том 10, № 7.
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abstract = "Moorea producens JHB, a Jamaican strain of tropical filamentous marine cyanobacteria, has been extensively studied by traditional natural products techniques. These previous bioassay and structure guided isolations led to the discovery of two exciting classes of natural products, hectochlorin (1) and jamaicamides A (2) and B (3). In the current study, mass spectrometry-based 'molecular networking' was used to visualize the metabolome of Moorea producens JHB, and both guided and enhanced the isolation workflow, revealing additional metabolites in these compound classes. Further, we developed additional insight into the metabolic capabilities of this strain by genome sequencing analysis, which subsequently led to the isolation of a compound unrelated to the jamaicamide and hectochlorin families. Another approach involved stimulation of the biosynthesis of a minor jamaicamide metabolite by cultivation in modified media, and provided insights about the underlying biosynthetic machinery as well as preliminary stru",
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Boudreau, PD, Monroe, EA, Mehrotra, S, Desfor, S, Korobeynikov, A, Sherman, DH, Murray, TF, Gerwick, L, Dorrestein, PC & Gerwick, WH 2015, 'Expanding the Described Metabolome of the Marine Cyanobacterium Moorea producens JHB through Orthogonal Natural Products Workflows', PLoS ONE, том. 10, № 7. https://doi.org/10.1371/journal.pone.0133297

Expanding the Described Metabolome of the Marine Cyanobacterium Moorea producens JHB through Orthogonal Natural Products Workflows. / Boudreau, P.D.; Monroe, E.A.; Mehrotra, S.; Desfor, S.; Korobeynikov, A.; Sherman, D.H.; Murray, T.F.; Gerwick, L.; Dorrestein, P.C.; Gerwick, W.H.

В: PLoS ONE, Том 10, № 7, 2015.

Результат исследований: Научные публикации в периодических изданияхстатья

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T1 - Expanding the Described Metabolome of the Marine Cyanobacterium Moorea producens JHB through Orthogonal Natural Products Workflows

AU - Boudreau, P.D.

AU - Monroe, E.A.

AU - Mehrotra, S.

AU - Desfor, S.

AU - Korobeynikov, A.

AU - Sherman, D.H.

AU - Murray, T.F.

AU - Gerwick, L.

AU - Dorrestein, P.C.

AU - Gerwick, W.H.

PY - 2015

Y1 - 2015

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