TY - JOUR
T1 - Evolutionary relationships of Metchnikovella dogieli Paskerova et al., 2016 (Microsporidia: Metchnikovellidae) revealed by multigene phylogenetic analysis
AU - Nassonova, Elena
AU - Bondarenko, Natalya
AU - Paskerova, Gita G.
AU - Kovacikova, Magdalena
AU - Frolova, Ekaterina V.
AU - Smirnov, Alexey
PY - 2021/1/8
Y1 - 2021/1/8
N2 - The species Metchnikovella dogieli (Paskerova et al. Protistology 10:148–157, 2016) belongs to one of the early diverging
microsporidian groups, the metchnikovellids (Microsporidia: Metchnikovellidae). In relation to typical (‘core’) microsporidia,
this group is considered primitive. The spores of metchnikovellids have no classical polar sac-anchoring disk complex, no coiled
polar tube, no posterior vacuole, and no polaroplast. Instead, they possess a short thick manubrium that expands into a manubrial
cistern. These organisms are hyperparasites; they infect gregarines that parasitise marine invertebrates. M. dogieli is a parasite of
the archigregarine Selenidium pygospionis (Paskerova et al. Protist 169:826–852, 2018), which parasitises the polychaete
Pygospio elegans. This species was discovered in samples collected in the silt littoral zone at the coast of the White Sea,
North-West Russia, and was described based on light microscopy. No molecular data are available for this species, and the
publicly accessible genomic data for metchnikovellids are limited to two species: M. incurvata Caullery & Mesnil, 1914 and
Amphiamblys sp. WSBS2006. In the present study, we applied single-cell genomics methods with whole-genome amplification
to perform next-generation sequencing of M. dogieli genomic DNA. We performed a phylogenetic analysis based on the SSU
rRNA gene and reconstructed a multigene phylogeny using a concatenated alignment that included 46 conserved single-copy
protein domains. The analyses recovered a fully supported clade of metchnikovellids as a basal group to the core microsporidia.
Two members of the genus Metchnikovella did not form a clade in our tree. This may indicate that this genus is paraphyletic and
requires revision.
AB - The species Metchnikovella dogieli (Paskerova et al. Protistology 10:148–157, 2016) belongs to one of the early diverging
microsporidian groups, the metchnikovellids (Microsporidia: Metchnikovellidae). In relation to typical (‘core’) microsporidia,
this group is considered primitive. The spores of metchnikovellids have no classical polar sac-anchoring disk complex, no coiled
polar tube, no posterior vacuole, and no polaroplast. Instead, they possess a short thick manubrium that expands into a manubrial
cistern. These organisms are hyperparasites; they infect gregarines that parasitise marine invertebrates. M. dogieli is a parasite of
the archigregarine Selenidium pygospionis (Paskerova et al. Protist 169:826–852, 2018), which parasitises the polychaete
Pygospio elegans. This species was discovered in samples collected in the silt littoral zone at the coast of the White Sea,
North-West Russia, and was described based on light microscopy. No molecular data are available for this species, and the
publicly accessible genomic data for metchnikovellids are limited to two species: M. incurvata Caullery & Mesnil, 1914 and
Amphiamblys sp. WSBS2006. In the present study, we applied single-cell genomics methods with whole-genome amplification
to perform next-generation sequencing of M. dogieli genomic DNA. We performed a phylogenetic analysis based on the SSU
rRNA gene and reconstructed a multigene phylogeny using a concatenated alignment that included 46 conserved single-copy
protein domains. The analyses recovered a fully supported clade of metchnikovellids as a basal group to the core microsporidia.
Two members of the genus Metchnikovella did not form a clade in our tree. This may indicate that this genus is paraphyletic and
requires revision.
KW - микроспоридии, мечниковеллиды, филогения, филогеномика
KW - Microsporidia
KW - Metchnikovellids
KW - Phylogeny
KW - Phylogenomics
UR - https://www.researchgate.net/publication/345842940_Evolutionary_relationships_of_Metchnikovella_dogieli_Paskerova_et_al_2016_Microsporidia_Metchnikovellidae_revealed_by_multigene_phylogenetic_analysis
M3 - Article
JO - Parasitology Research
JF - Parasitology Research
SN - 0932-0113
ER -