Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae

A. S. Zhuk, E. I. Stepchenkova, Y. I. Pavlov, S. G. Inge-Vechtomov

Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

Выдержка

Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4).

Язык оригиналаанглийский
Страницы (с-по)111-122
Число страниц12
ЖурналCell and Tissue Biology
Том11
Номер выпуска2
DOI
СостояниеОпубликовано - 1 мар 2017
Опубликовано для внешнего пользованияДа

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    • Клеточная биология

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    title = "Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae",
    abstract = "Synchronization of cell division in yeast cultures of Saccharomyces cerevisiae is widely used in studies on regulation of eukaryotic gene expression and biochemical processes at different stages of the cell cycle. In this study, we compared the efficacy of modern widely used methodologies to achieve and assess the degree of synchronization of cell division in yeast. Based on the literature and our own data, we propose practical recommendations for synchronization of cell divisions in S. cerevisiae using chemical reagents (alpha-factor, hydroxyurea, nocodazole), and a genetic cell-cycle block (temperature-sensitive mutation cdc28-4).",
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    Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae. / Zhuk, A. S.; Stepchenkova, E. I.; Pavlov, Y. I.; Inge-Vechtomov, S. G.

    В: Cell and Tissue Biology, Том 11, № 2, 01.03.2017, стр. 111-122.

    Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

    TY - JOUR

    T1 - Evaluation of methods of synchronization of cell division in yeast Saccharomyces cerevisiae

    AU - Zhuk, A. S.

    AU - Stepchenkova, E. I.

    AU - Pavlov, Y. I.

    AU - Inge-Vechtomov, S. G.

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