Effect of mutations in genes PH085 and PH04 on proline utilization in yeast Saccharomyces cerevisiae

Результат исследований: Научные публикации в периодических изданияхстатья

3 Цитирования (Scopus)

Выдержка

Yeast Saccharomyces cerevisiae can utilize proline as a single nitrogen source. We have found that the strains with mutations in gene PH085, a structural gene encoding cyclin-dependent phosphoprotein kinase, cannot grow on the proline-containing media. The ability to utilize proline is restored in strains of the pho85pho4 genotype. We suggest that phosphoprotein kinase Pho85p is involved in either phosphprylation of a highly specific proline permease, Put4p, or in signaling proline concentration. The Pho4p protein that activates transcription of the PH05 gene, a structural gene of acid phosphatase, seems to participate in the negative regulation of the PUT1 and PUT2 genes encoding enzymes of proline catabolism, proline oxidase and Δ-pyrroline-5-carboxylate dehydrogenase. Thus, regulation of phosphorus and nitrogen metabolism have common elements.

Язык оригиналаанглийский
Страницы (с-по)1364-1369
Число страниц6
ЖурналRussian Journal of Genetics
Том36
Номер выпуска12
DOI
СостояниеОпубликовано - 1 янв 2000

Отпечаток

Proline
Saccharomyces cerevisiae
Yeasts
Mutation
Genes
Phosphoproteins
Proline Oxidase
Nitrogen
Cyclin-Dependent Kinases
Acid Phosphatase
Phosphorus
Oxidoreductases
Phosphotransferases
Genotype
Enzymes
Proteins

Предметные области Scopus

  • Генетика

Цитировать

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title = "Effect of mutations in genes PH085 and PH04 on proline utilization in yeast Saccharomyces cerevisiae",
abstract = "Yeast Saccharomyces cerevisiae can utilize proline as a single nitrogen source. We have found that the strains with mutations in gene PH085, a structural gene encoding cyclin-dependent phosphoprotein kinase, cannot grow on the proline-containing media. The ability to utilize proline is restored in strains of the pho85pho4 genotype. We suggest that phosphoprotein kinase Pho85p is involved in either phosphprylation of a highly specific proline permease, Put4p, or in signaling proline concentration. The Pho4p protein that activates transcription of the PH05 gene, a structural gene of acid phosphatase, seems to participate in the negative regulation of the PUT1 and PUT2 genes encoding enzymes of proline catabolism, proline oxidase and Δ-pyrroline-5-carboxylate dehydrogenase. Thus, regulation of phosphorus and nitrogen metabolism have common elements.",
author = "Popova, {Yu G.} and Padkina, {M. V.} and Sambuk, {E. V.}",
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Effect of mutations in genes PH085 and PH04 on proline utilization in yeast Saccharomyces cerevisiae. / Popova, Yu G.; Padkina, M. V.; Sambuk, E. V.

В: Russian Journal of Genetics, Том 36, № 12, 01.01.2000, стр. 1364-1369.

Результат исследований: Научные публикации в периодических изданияхстатья

TY - JOUR

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N2 - Yeast Saccharomyces cerevisiae can utilize proline as a single nitrogen source. We have found that the strains with mutations in gene PH085, a structural gene encoding cyclin-dependent phosphoprotein kinase, cannot grow on the proline-containing media. The ability to utilize proline is restored in strains of the pho85pho4 genotype. We suggest that phosphoprotein kinase Pho85p is involved in either phosphprylation of a highly specific proline permease, Put4p, or in signaling proline concentration. The Pho4p protein that activates transcription of the PH05 gene, a structural gene of acid phosphatase, seems to participate in the negative regulation of the PUT1 and PUT2 genes encoding enzymes of proline catabolism, proline oxidase and Δ-pyrroline-5-carboxylate dehydrogenase. Thus, regulation of phosphorus and nitrogen metabolism have common elements.

AB - Yeast Saccharomyces cerevisiae can utilize proline as a single nitrogen source. We have found that the strains with mutations in gene PH085, a structural gene encoding cyclin-dependent phosphoprotein kinase, cannot grow on the proline-containing media. The ability to utilize proline is restored in strains of the pho85pho4 genotype. We suggest that phosphoprotein kinase Pho85p is involved in either phosphprylation of a highly specific proline permease, Put4p, or in signaling proline concentration. The Pho4p protein that activates transcription of the PH05 gene, a structural gene of acid phosphatase, seems to participate in the negative regulation of the PUT1 and PUT2 genes encoding enzymes of proline catabolism, proline oxidase and Δ-pyrroline-5-carboxylate dehydrogenase. Thus, regulation of phosphorus and nitrogen metabolism have common elements.

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