TY - JOUR

T1 - Does protein glycation impact on the drought-related changes in metabolism and nutritional properties of mature pea (Pisum sativum L.) seeds?

AU - Leonova, Tatiana

AU - Popova, Veronika

AU - Tsarev, Alexander

AU - Henning, Christian

AU - Antonova, Kristina

AU - Rogovskaya, Nadezhda

AU - Vikhnina, Maria

AU - Baldensperger, Tim

AU - Soboleva, Alena

AU - Dinastia, Ekaterina

AU - Dorn, Mandy

AU - Shiroglasova, Olga

AU - Grishina, Tatiana

AU - Balcke, Gerd U.

AU - Ihling, Christian

AU - Smolikova, Galina

AU - Medvedev, Sergei

AU - Zhukov, Vladimir A.

AU - Babakov, Vladimir

AU - Tikhonovich, Igor A.

AU - Glomb, Marcus A.

AU - Bilova, Tatiana

AU - Frolov, Andrej

PY - 2020/1/2

Y1 - 2020/1/2

N2 - Protein glycation is usually referred to as an array of non-enzymatic post-translational modifications formed by reducing sugars and carbonyl products of their degradation. The resulting advanced glycation end products (AGEs) represent a heterogeneous group of covalent adducts, known for their pro-inflammatory effects in mammals, and impacting on pathogenesis of metabolic diseases and ageing. In plants, AGEs are the markers of tissue ageing and response to environmental stressors, the most prominent of which is drought. Although water deficit enhances protein glycation in leaves, its effect on seed glycation profiles is still unknown. Moreover, the effect of drought on biological activities of seed protein in mammalian systems is still unstudied with respect to glycation. Therefore, here we address the effects of a short-term drought on the patterns of seed protein-bound AGEs and accompanying alterations in pro-inflammatory properties of seed protein in the context of seed metabolome dynamics. A short-term drought, simulated as polyethylene glycol-induced osmotic stress and applied at the stage of seed filling, resulted in the dramatic suppression of primary seed metabolism, although the secondary metabolome was minimally affected. This was accompanied with significant suppression of NF-kB activation in human SH-SY5Y neuroblastoma cells after a treatment with protein hydrolyzates, isolated from the mature seeds of drought-treated plants. This effect could not be attributed to formation of known AGEs. Most likely, the prospective anti-inflammatory effect of short-term drought is related to antioxidant effect of unknown secondary metabolite protein adducts, or down-regulation of unknown plant-specific AGEs due to suppression of energy metabolism during seed filling.

AB - Protein glycation is usually referred to as an array of non-enzymatic post-translational modifications formed by reducing sugars and carbonyl products of their degradation. The resulting advanced glycation end products (AGEs) represent a heterogeneous group of covalent adducts, known for their pro-inflammatory effects in mammals, and impacting on pathogenesis of metabolic diseases and ageing. In plants, AGEs are the markers of tissue ageing and response to environmental stressors, the most prominent of which is drought. Although water deficit enhances protein glycation in leaves, its effect on seed glycation profiles is still unknown. Moreover, the effect of drought on biological activities of seed protein in mammalian systems is still unstudied with respect to glycation. Therefore, here we address the effects of a short-term drought on the patterns of seed protein-bound AGEs and accompanying alterations in pro-inflammatory properties of seed protein in the context of seed metabolome dynamics. A short-term drought, simulated as polyethylene glycol-induced osmotic stress and applied at the stage of seed filling, resulted in the dramatic suppression of primary seed metabolism, although the secondary metabolome was minimally affected. This was accompanied with significant suppression of NF-kB activation in human SH-SY5Y neuroblastoma cells after a treatment with protein hydrolyzates, isolated from the mature seeds of drought-treated plants. This effect could not be attributed to formation of known AGEs. Most likely, the prospective anti-inflammatory effect of short-term drought is related to antioxidant effect of unknown secondary metabolite protein adducts, or down-regulation of unknown plant-specific AGEs due to suppression of energy metabolism during seed filling.

KW - Advanced glycation end products (AGEs)

KW - Drought

KW - Glycation

KW - Metabolomics

KW - Osmotic stress

KW - Pea (Pisum sativum L.)

KW - Pro-inflammatory

KW - Seed metabolism

KW - Seeds

KW - SH-SY5Y human neuroblastoma cells

KW - Signaling pathways

UR - http://www.scopus.com/inward/record.url?scp=85078021391&partnerID=8YFLogxK

U2 - 10.3390/ijms21020567

DO - 10.3390/ijms21020567

M3 - Article

C2 - 31952342

AN - SCOPUS:85078021391

VL - 21

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 2

M1 - 567

ER -