Determination of protein adducts of organophosphorus nerve agents in blood plasma

N. L. Koryagina, E. I. Savel’eva, G. V. Karakashev, V. N. Babakov, Ya A. Dubrovskii, E. S. Ukolova, N. S. Khlebnikova, E. A. Murashko, V. Yu Koneva, A. I. Ukolov, V. A. Kopeikin, A. S. Radilov

Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

5 Цитирования (Scopus)

Выдержка

A comparative study of the efficiency of procedures for the determination of the biomarkers of organophosphorus agents (OPAs) in blood plasma was performed. It was found that the gas chromatography–mass spectrometry determination of OPAs reactivated from the composition of protein adducts is a rapid method for the detection of exposure to OPAs. The liquid chromatography–mass spectrometry determination of phosphonylated butyrylcholinesterase and albumin fragments modified with OPA residues provides an opportunity to perform more sensitive and retrospective analysis. The tyrosine adducts of OPAs with serum albumin and other blood plasma proteins are not prone to dealkylation in the course of aging; in the series of the test markers, they possess the greatest diagnostic value because they make it possible to determine the precise structure a toxic agent after the longest time interval after exposure. The tentative limit of detection of OPA markers varies from 0.1 to 1.0 ng/mL.

Язык оригиналаанглийский
Страницы (с-по)849-859
Число страниц11
ЖурналJournal of Analytical Chemistry
Том71
Номер выпуска8
DOI
СостояниеОпубликовано - 1 авг 2016

Предметные области Scopus

  • Аналитическая химия

Цитировать

Koryagina, N. L., Savel’eva, E. I., Karakashev, G. V., Babakov, V. N., Dubrovskii, Y. A., Ukolova, E. S., ... Radilov, A. S. (2016). Determination of protein adducts of organophosphorus nerve agents in blood plasma. Journal of Analytical Chemistry, 71(8), 849-859. https://doi.org/10.1134/S1061934816080086
Koryagina, N. L. ; Savel’eva, E. I. ; Karakashev, G. V. ; Babakov, V. N. ; Dubrovskii, Ya A. ; Ukolova, E. S. ; Khlebnikova, N. S. ; Murashko, E. A. ; Koneva, V. Yu ; Ukolov, A. I. ; Kopeikin, V. A. ; Radilov, A. S. / Determination of protein adducts of organophosphorus nerve agents in blood plasma. В: Journal of Analytical Chemistry. 2016 ; Том 71, № 8. стр. 849-859.
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abstract = "A comparative study of the efficiency of procedures for the determination of the biomarkers of organophosphorus agents (OPAs) in blood plasma was performed. It was found that the gas chromatography–mass spectrometry determination of OPAs reactivated from the composition of protein adducts is a rapid method for the detection of exposure to OPAs. The liquid chromatography–mass spectrometry determination of phosphonylated butyrylcholinesterase and albumin fragments modified with OPA residues provides an opportunity to perform more sensitive and retrospective analysis. The tyrosine adducts of OPAs with serum albumin and other blood plasma proteins are not prone to dealkylation in the course of aging; in the series of the test markers, they possess the greatest diagnostic value because they make it possible to determine the precise structure a toxic agent after the longest time interval after exposure. The tentative limit of detection of OPA markers varies from 0.1 to 1.0 ng/mL.",
keywords = "blood plasma, gas chromatography, HPLC, markers of exposure, mass-spectrometric detection, organophosphorus agents, retrospective analysis",
author = "Koryagina, {N. L.} and Savel’eva, {E. I.} and Karakashev, {G. V.} and Babakov, {V. N.} and Dubrovskii, {Ya A.} and Ukolova, {E. S.} and Khlebnikova, {N. S.} and Murashko, {E. A.} and Koneva, {V. Yu} and Ukolov, {A. I.} and Kopeikin, {V. A.} and Radilov, {A. S.}",
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Koryagina, NL, Savel’eva, EI, Karakashev, GV, Babakov, VN, Dubrovskii, YA, Ukolova, ES, Khlebnikova, NS, Murashko, EA, Koneva, VY, Ukolov, AI, Kopeikin, VA & Radilov, AS 2016, 'Determination of protein adducts of organophosphorus nerve agents in blood plasma', Journal of Analytical Chemistry, том. 71, № 8, стр. 849-859. https://doi.org/10.1134/S1061934816080086

Determination of protein adducts of organophosphorus nerve agents in blood plasma. / Koryagina, N. L.; Savel’eva, E. I.; Karakashev, G. V.; Babakov, V. N.; Dubrovskii, Ya A.; Ukolova, E. S.; Khlebnikova, N. S.; Murashko, E. A.; Koneva, V. Yu; Ukolov, A. I.; Kopeikin, V. A.; Radilov, A. S.

В: Journal of Analytical Chemistry, Том 71, № 8, 01.08.2016, стр. 849-859.

Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

TY - JOUR

T1 - Determination of protein adducts of organophosphorus nerve agents in blood plasma

AU - Koryagina, N. L.

AU - Savel’eva, E. I.

AU - Karakashev, G. V.

AU - Babakov, V. N.

AU - Dubrovskii, Ya A.

AU - Ukolova, E. S.

AU - Khlebnikova, N. S.

AU - Murashko, E. A.

AU - Koneva, V. Yu

AU - Ukolov, A. I.

AU - Kopeikin, V. A.

AU - Radilov, A. S.

PY - 2016/8/1

Y1 - 2016/8/1

N2 - A comparative study of the efficiency of procedures for the determination of the biomarkers of organophosphorus agents (OPAs) in blood plasma was performed. It was found that the gas chromatography–mass spectrometry determination of OPAs reactivated from the composition of protein adducts is a rapid method for the detection of exposure to OPAs. The liquid chromatography–mass spectrometry determination of phosphonylated butyrylcholinesterase and albumin fragments modified with OPA residues provides an opportunity to perform more sensitive and retrospective analysis. The tyrosine adducts of OPAs with serum albumin and other blood plasma proteins are not prone to dealkylation in the course of aging; in the series of the test markers, they possess the greatest diagnostic value because they make it possible to determine the precise structure a toxic agent after the longest time interval after exposure. The tentative limit of detection of OPA markers varies from 0.1 to 1.0 ng/mL.

AB - A comparative study of the efficiency of procedures for the determination of the biomarkers of organophosphorus agents (OPAs) in blood plasma was performed. It was found that the gas chromatography–mass spectrometry determination of OPAs reactivated from the composition of protein adducts is a rapid method for the detection of exposure to OPAs. The liquid chromatography–mass spectrometry determination of phosphonylated butyrylcholinesterase and albumin fragments modified with OPA residues provides an opportunity to perform more sensitive and retrospective analysis. The tyrosine adducts of OPAs with serum albumin and other blood plasma proteins are not prone to dealkylation in the course of aging; in the series of the test markers, they possess the greatest diagnostic value because they make it possible to determine the precise structure a toxic agent after the longest time interval after exposure. The tentative limit of detection of OPA markers varies from 0.1 to 1.0 ng/mL.

KW - blood plasma

KW - gas chromatography

KW - HPLC

KW - markers of exposure

KW - mass-spectrometric detection

KW - organophosphorus agents

KW - retrospective analysis

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U2 - 10.1134/S1061934816080086

DO - 10.1134/S1061934816080086

M3 - Article

VL - 71

SP - 849

EP - 859

JO - Journal of Analytical Chemistry

JF - Journal of Analytical Chemistry

SN - 1061-9348

IS - 8

ER -

Koryagina NL, Savel’eva EI, Karakashev GV, Babakov VN, Dubrovskii YA, Ukolova ES и соавт. Determination of protein adducts of organophosphorus nerve agents in blood plasma. Journal of Analytical Chemistry. 2016 Авг. 1;71(8):849-859. https://doi.org/10.1134/S1061934816080086