Comparative genomics uncovers the prolific and distinctive metabolic potential of the cyanobacterial genus Moorea

Tiago Leao, Guilherme Castelão, Anton Korobeynikov, Emily A. Monroe, Sheila Podell, Evgenia Glukhov, Eric E. Allen, William H. Gerwick, Lena Gerwick

Результат исследований: Научные публикации в периодических изданияхстатья

20 Цитирования (Scopus)

Выдержка

Cyanobacteria are major sources of oxygen, nitrogen, and carbon in nature. In addition to the importance of their primary metabolism, some cyanobacteria are prolific producers of unique and bioactive secondary metabolites. Chemical investigations of the cyanobacterial genus Moorea have resulted in the isolation of over 190 compounds in the last two decades. However, preliminary genomic analysis has suggested that genome-guided approaches can enable the discovery of novel compounds from even well-studied Moorea strains, highlighting the importance of obtaining complete genomes. We report a complete genome of a filamentous tropical marine cyanobacterium, Moorea producens PAL, which reveals that about one-fifth of its genome is devoted to production of secondary metabolites, an impressive four times the cyanobacterial average. Moreover, possession of the complete PAL genome has allowed improvement to the assembly of three other Moorea draft genomes. Comparative genomics revealed that they are remarkably similar to one another, despite their differences in geography,morphology, and secondary metabolite profiles. Gene cluster networking highlights that this genus is distinctive among cyanobacteria, not only in the number of secondary metabolite pathways but also in the content of many pathways, which are potentially distinct from all other bacterial gene clusters to date. These findings portend that future genome-guided secondary metabolite discovery and isolation efforts should be highly productive.

Язык оригиналаанглийский
Страницы (с-по)3198-3203
Число страниц6
ЖурналProceedings of the National Academy of Sciences of the United States of America
Том114
Номер выпуска12
DOI
СостояниеОпубликовано - 21 мар 2017

Отпечаток

genomics
secondary metabolites
genome
Cyanobacteria
multigene family
Moorea producens
geography
Moorea
oxygen
metabolism
carbon
nitrogen

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Leao, Tiago ; Castelão, Guilherme ; Korobeynikov, Anton ; Monroe, Emily A. ; Podell, Sheila ; Glukhov, Evgenia ; Allen, Eric E. ; Gerwick, William H. ; Gerwick, Lena. / Comparative genomics uncovers the prolific and distinctive metabolic potential of the cyanobacterial genus Moorea. В: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Том 114, № 12. стр. 3198-3203.
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abstract = "Cyanobacteria are major sources of oxygen, nitrogen, and carbon in nature. In addition to the importance of their primary metabolism, some cyanobacteria are prolific producers of unique and bioactive secondary metabolites. Chemical investigations of the cyanobacterial genus Moorea have resulted in the isolation of over 190 compounds in the last two decades. However, preliminary genomic analysis has suggested that genome-guided approaches can enable the discovery of novel compounds from even well-studied Moorea strains, highlighting the importance of obtaining complete genomes. We report a complete genome of a filamentous tropical marine cyanobacterium, Moorea producens PAL, which reveals that about one-fifth of its genome is devoted to production of secondary metabolites, an impressive four times the cyanobacterial average. Moreover, possession of the complete PAL genome has allowed improvement to the assembly of three other Moorea draft genomes. Comparative genomics revealed that they are remarkably similar to one another, despite their differences in geography,morphology, and secondary metabolite profiles. Gene cluster networking highlights that this genus is distinctive among cyanobacteria, not only in the number of secondary metabolite pathways but also in the content of many pathways, which are potentially distinct from all other bacterial gene clusters to date. These findings portend that future genome-guided secondary metabolite discovery and isolation efforts should be highly productive.",
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Comparative genomics uncovers the prolific and distinctive metabolic potential of the cyanobacterial genus Moorea. / Leao, Tiago; Castelão, Guilherme; Korobeynikov, Anton; Monroe, Emily A.; Podell, Sheila; Glukhov, Evgenia; Allen, Eric E.; Gerwick, William H.; Gerwick, Lena.

В: Proceedings of the National Academy of Sciences of the United States of America, Том 114, № 12, 21.03.2017, стр. 3198-3203.

Результат исследований: Научные публикации в периодических изданияхстатья

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T1 - Comparative genomics uncovers the prolific and distinctive metabolic potential of the cyanobacterial genus Moorea

AU - Leao, Tiago

AU - Castelão, Guilherme

AU - Korobeynikov, Anton

AU - Monroe, Emily A.

AU - Podell, Sheila

AU - Glukhov, Evgenia

AU - Allen, Eric E.

AU - Gerwick, William H.

AU - Gerwick, Lena

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N2 - Cyanobacteria are major sources of oxygen, nitrogen, and carbon in nature. In addition to the importance of their primary metabolism, some cyanobacteria are prolific producers of unique and bioactive secondary metabolites. Chemical investigations of the cyanobacterial genus Moorea have resulted in the isolation of over 190 compounds in the last two decades. However, preliminary genomic analysis has suggested that genome-guided approaches can enable the discovery of novel compounds from even well-studied Moorea strains, highlighting the importance of obtaining complete genomes. We report a complete genome of a filamentous tropical marine cyanobacterium, Moorea producens PAL, which reveals that about one-fifth of its genome is devoted to production of secondary metabolites, an impressive four times the cyanobacterial average. Moreover, possession of the complete PAL genome has allowed improvement to the assembly of three other Moorea draft genomes. Comparative genomics revealed that they are remarkably similar to one another, despite their differences in geography,morphology, and secondary metabolite profiles. Gene cluster networking highlights that this genus is distinctive among cyanobacteria, not only in the number of secondary metabolite pathways but also in the content of many pathways, which are potentially distinct from all other bacterial gene clusters to date. These findings portend that future genome-guided secondary metabolite discovery and isolation efforts should be highly productive.

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KW - Gene cluster network

KW - Genome comparison

KW - Heterocyst glycolipids

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