Comparative characteristics of the stem cells isolated from subcutaneous and subepicardial adipose tissue

T.A. Krylova, O.A. Bystrova, A.A. Khudyakov, A.B. Malashicheva, O.M. Moiseeva, V.V. Zenin, M.G. Martynova

Результат исследований: Научные публикации в периодических изданияхстатья

1 Цитирования (Scopus)


Background. Stem cells (SCs) considerably vary in morphological, immunophenotypic, proliferative, and differentiation characteristics depending on their tissue source. The comparative analysis of their biological properties is essential for the optimal choice of SCs for regenerative therapies. Methods. Using immunocytochemistry, flow cytometry, histochemistry and real-time RT-PCR, we have investigated SCs obtained from human subepicardial (SEC-AT) and subcutaneous (SC-AT) adipose tissue and cultured under similar culture conditions without any differentiation-promoting factors. Results. The cultures were similar in the high proportion of proliferating cell nuclear antigen (PCNA)-positive cells. In both cultures, immunophenotyping has revealed high expression of mesenchymal stem cell surface markers CD29, CD44, CD73, and CD105, low expression of CD31, CD34 and CD45, and wide variability in CD117, CD146 and CD309 expression. The only distinction in CD marker profile was significantly lower expression of CD90 in
Язык оригиналарусский
Страницы (с-по)212-217
Номер выпуска3
СостояниеОпубликовано - 2014