Bosea caraganae sp. nov. a new species of slow-growing rhizobia isolated from root nodules of the relict species Caragana jubata (Pall.) Poir. originating from Mongolia

A. L. Sazanova, V.I. Safronova, I.G. Kuznetsova, D. S. Karlov, A. A. Belimov, E.E. Andronov, E.R. Chirak, J.P. Popova, A.V. Verkhozina, A. Willems, I.A. Tikhonovich

Результат исследований: Научные публикации в периодических изданияхстатья

Выдержка

Two Gram-stain-negative strains, RCAM04680T and RCAM04685, were isolated from root nodules of the relict legume Caragana jubata (Pall.) Poir. originating from the south-western shore of Lake Khuvsgul (Mongolia). The 16S rRNA gene (rrs) sequencing data showed that these novel isolates belong to the genus Bosea and are phylogenetically closest to the type strains Bosea lathyri LMG 26379T, Bosea vaviloviae LMG 28367T, Bosea massiliensis LMG 26221T and Bosea lupini LMG 26383T (the rrs-similarity levels were 98.7-98.8 %). The recA gene of strain RCAM04680T showed the highest sequence similarity to the type strain B. lupini LMG 26383T (95.4 %), while its atpD gene was closest to that of B. lathyri LMG 26379T (94.4 %). The ITS, dnaK and gyrB sequences of this isolate were most similar to the B. vaviloviae LMG 28367T (86.8 % for ITS, 90.4 % for the other genes). The most abundant fatty acid was C18 : 1ω7c (40.8 %). The whole genomes of strains RCAM04680T and RCAM04685 were identical (100 % average nucleotide identity). The highest average nucleotide identity value (82.8 %) was found between the genome of strain RCAM04680T and B. vaviloviae LMG 28367T. The common nodABC genes required for legume nodulation were absent in both strains; however, some other symbiotic nol, nod, nif and fix genes were detected. Based on the genetic study, as well as analyses of the whole-cell fatty acid compositions and phenotypic properties, a new species, Boseacaraganae sp. nov. (type strain RCAM04680T (=LMG 31125T), is proposed.
Язык оригиналаанглийский
Страницы (с-по)2687-2695
ЖурналInternational Journal of Systematic and Evolutionary Microbiology
Том69
Номер выпуска9
СостояниеОпубликовано - 2019

Отпечаток

Caragana
Bosea
relict species
Mongolia
Rhizobium
root nodules
rhizobacterium
new species
gene
Genes
Fabaceae
Fatty Acids
Nucleotides
genes
Genome
fatty acid
genome
Lakes
rRNA Genes
legumes

Цитировать

Sazanova, A. L. ; Safronova, V.I. ; Kuznetsova, I.G. ; Karlov, D. S. ; Belimov, A. A. ; Andronov, E.E. ; Chirak, E.R. ; Popova, J.P. ; Verkhozina, A.V. ; Willems, A. ; Tikhonovich, I.A. / Bosea caraganae sp. nov. a new species of slow-growing rhizobia isolated from root nodules of the relict species Caragana jubata (Pall.) Poir. originating from Mongolia. В: International Journal of Systematic and Evolutionary Microbiology. 2019 ; Том 69, № 9. стр. 2687-2695.
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title = "Bosea caraganae sp. nov. a new species of slow-growing rhizobia isolated from root nodules of the relict species Caragana jubata (Pall.) Poir. originating from Mongolia",
abstract = "Two Gram-stain-negative strains, RCAM04680T and RCAM04685, were isolated from root nodules of the relict legume Caragana jubata (Pall.) Poir. originating from the south-western shore of Lake Khuvsgul (Mongolia). The 16S rRNA gene (rrs) sequencing data showed that these novel isolates belong to the genus Bosea and are phylogenetically closest to the type strains Bosea lathyri LMG 26379T, Bosea vaviloviae LMG 28367T, Bosea massiliensis LMG 26221T and Bosea lupini LMG 26383T (the rrs-similarity levels were 98.7-98.8 {\%}). The recA gene of strain RCAM04680T showed the highest sequence similarity to the type strain B. lupini LMG 26383T (95.4 {\%}), while its atpD gene was closest to that of B. lathyri LMG 26379T (94.4 {\%}). The ITS, dnaK and gyrB sequences of this isolate were most similar to the B. vaviloviae LMG 28367T (86.8 {\%} for ITS, 90.4 {\%} for the other genes). The most abundant fatty acid was C18 : 1ω7c (40.8 {\%}). The whole genomes of strains RCAM04680T and RCAM04685 were identical (100 {\%} average nucleotide identity). The highest average nucleotide identity value (82.8 {\%}) was found between the genome of strain RCAM04680T and B. vaviloviae LMG 28367T. The common nodABC genes required for legume nodulation were absent in both strains; however, some other symbiotic nol, nod, nif and fix genes were detected. Based on the genetic study, as well as analyses of the whole-cell fatty acid compositions and phenotypic properties, a new species, Boseacaraganae sp. nov. (type strain RCAM04680T (=LMG 31125T), is proposed.",
keywords = "Bosea caraganae sp. nov., relict legumes, Root nodule bacteria",
author = "Sazanova, {A. L.} and V.I. Safronova and I.G. Kuznetsova and Karlov, {D. S.} and Belimov, {A. A.} and E.E. Andronov and E.R. Chirak and J.P. Popova and A.V. Verkhozina and A. Willems and I.A. Tikhonovich",
year = "2019",
language = "English",
volume = "69",
pages = "2687--2695",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "9",

}

Sazanova, AL, Safronova, VI, Kuznetsova, IG, Karlov, DS, Belimov, AA, Andronov, EE, Chirak, ER, Popova, JP, Verkhozina, AV, Willems, A & Tikhonovich, IA 2019, 'Bosea caraganae sp. nov. a new species of slow-growing rhizobia isolated from root nodules of the relict species Caragana jubata (Pall.) Poir. originating from Mongolia', International Journal of Systematic and Evolutionary Microbiology, том. 69, № 9, стр. 2687-2695.

Bosea caraganae sp. nov. a new species of slow-growing rhizobia isolated from root nodules of the relict species Caragana jubata (Pall.) Poir. originating from Mongolia. / Sazanova, A. L.; Safronova, V.I.; Kuznetsova, I.G.; Karlov, D. S.; Belimov, A. A.; Andronov, E.E.; Chirak, E.R.; Popova, J.P.; Verkhozina, A.V.; Willems, A.; Tikhonovich, I.A.

В: International Journal of Systematic and Evolutionary Microbiology, Том 69, № 9, 2019, стр. 2687-2695.

Результат исследований: Научные публикации в периодических изданияхстатья

TY - JOUR

T1 - Bosea caraganae sp. nov. a new species of slow-growing rhizobia isolated from root nodules of the relict species Caragana jubata (Pall.) Poir. originating from Mongolia

AU - Sazanova, A. L.

AU - Safronova, V.I.

AU - Kuznetsova, I.G.

AU - Karlov, D. S.

AU - Belimov, A. A.

AU - Andronov, E.E.

AU - Chirak, E.R.

AU - Popova, J.P.

AU - Verkhozina, A.V.

AU - Willems, A.

AU - Tikhonovich, I.A.

PY - 2019

Y1 - 2019

N2 - Two Gram-stain-negative strains, RCAM04680T and RCAM04685, were isolated from root nodules of the relict legume Caragana jubata (Pall.) Poir. originating from the south-western shore of Lake Khuvsgul (Mongolia). The 16S rRNA gene (rrs) sequencing data showed that these novel isolates belong to the genus Bosea and are phylogenetically closest to the type strains Bosea lathyri LMG 26379T, Bosea vaviloviae LMG 28367T, Bosea massiliensis LMG 26221T and Bosea lupini LMG 26383T (the rrs-similarity levels were 98.7-98.8 %). The recA gene of strain RCAM04680T showed the highest sequence similarity to the type strain B. lupini LMG 26383T (95.4 %), while its atpD gene was closest to that of B. lathyri LMG 26379T (94.4 %). The ITS, dnaK and gyrB sequences of this isolate were most similar to the B. vaviloviae LMG 28367T (86.8 % for ITS, 90.4 % for the other genes). The most abundant fatty acid was C18 : 1ω7c (40.8 %). The whole genomes of strains RCAM04680T and RCAM04685 were identical (100 % average nucleotide identity). The highest average nucleotide identity value (82.8 %) was found between the genome of strain RCAM04680T and B. vaviloviae LMG 28367T. The common nodABC genes required for legume nodulation were absent in both strains; however, some other symbiotic nol, nod, nif and fix genes were detected. Based on the genetic study, as well as analyses of the whole-cell fatty acid compositions and phenotypic properties, a new species, Boseacaraganae sp. nov. (type strain RCAM04680T (=LMG 31125T), is proposed.

AB - Two Gram-stain-negative strains, RCAM04680T and RCAM04685, were isolated from root nodules of the relict legume Caragana jubata (Pall.) Poir. originating from the south-western shore of Lake Khuvsgul (Mongolia). The 16S rRNA gene (rrs) sequencing data showed that these novel isolates belong to the genus Bosea and are phylogenetically closest to the type strains Bosea lathyri LMG 26379T, Bosea vaviloviae LMG 28367T, Bosea massiliensis LMG 26221T and Bosea lupini LMG 26383T (the rrs-similarity levels were 98.7-98.8 %). The recA gene of strain RCAM04680T showed the highest sequence similarity to the type strain B. lupini LMG 26383T (95.4 %), while its atpD gene was closest to that of B. lathyri LMG 26379T (94.4 %). The ITS, dnaK and gyrB sequences of this isolate were most similar to the B. vaviloviae LMG 28367T (86.8 % for ITS, 90.4 % for the other genes). The most abundant fatty acid was C18 : 1ω7c (40.8 %). The whole genomes of strains RCAM04680T and RCAM04685 were identical (100 % average nucleotide identity). The highest average nucleotide identity value (82.8 %) was found between the genome of strain RCAM04680T and B. vaviloviae LMG 28367T. The common nodABC genes required for legume nodulation were absent in both strains; however, some other symbiotic nol, nod, nif and fix genes were detected. Based on the genetic study, as well as analyses of the whole-cell fatty acid compositions and phenotypic properties, a new species, Boseacaraganae sp. nov. (type strain RCAM04680T (=LMG 31125T), is proposed.

KW - Bosea caraganae sp. nov.

KW - relict legumes

KW - Root nodule bacteria

UR - https://www.ncbi.nlm.nih.gov/pubmed/31166161

M3 - Article

VL - 69

SP - 2687

EP - 2695

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 9

ER -