Biological response of chemically treated surface of the ultrafine-grained Ti-6Al-7Nb alloy for biomedical applications

Diego Pedreira de Oliveira, Tatiane Venturott Toniato, Ritchelli Ricci, Fernanda Roberta Marciano, Egor Prokofiev, Ruslan Z. Valiev, Anderson Oliveira Lobo, Alberto Moreira Jorge Júnior

Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

Выдержка

Background: Nanophase surface properties of titanium alloys must be obtained for a suitable biological performance, particularly to facilitate cell adhesion and bone tissue formation. Obtaining a bulk nanostructured material using severe plastic deformation is an ideal processing route to improve the mechanical performance of titanium alloys. By decreasing the grain size of a metallic material, a superior strength improvement can be obtained, while surface modification of a nanostructured surface can produce an attractive topography able to induce biological responses in osteoblastic cells. Methods: Aiming to achieve such an excellent synergetic performance, a processing route, which included equal channel angular pressing (ECAP), hot and cold extrusion, and heat treatments, was used to produce a nanometric and ultrafine-grained (UFG) microstructure in the Ti-6Al-7Nb alloy (around of 200 nm). Additionally, UFG samples were surface-modified with acid etching (UFG-A) to produce a uniform micron and submicron porosity on the surface. Subsequently, alkaline treatment (UFG-AA) produced a sponge-like nanotopographic substrate able to modulate cellular interactions. Results: After several kinds of biological tests for both treatment conditions (UFG-A and UFG-AA), the main results have shown that there was no cytotoxicity, expressed alkaline phosphatase activity and total protein amounts without statistical differences compared to control. However, the UFG-AA samples presented an attractive effect on the cell membranes, and cell adhesions were preferentially induced as compared with UFG-A. Both conditions demonstrated cell projections, but for UFG-AA, cells were more widely dispersed, and more quantities of filopodia formation could be observed. Conclusion: Herein, the reasons for such behaviors are discussed, and further results are presented in addition to those mentioned above.

Язык оригиналаанглийский
Страницы (с-по)1725-1736
Число страниц12
ЖурналInternational Journal of Nanomedicine
Том14
DOI
СостояниеОпубликовано - 1 янв 2019

Предметные области Scopus

  • Биофизика
  • Биоинженерия
  • Биоматериалы
  • Фармация
  • Поиск новых лекарств
  • Органическая химия

Цитировать

de Oliveira, Diego Pedreira ; Toniato, Tatiane Venturott ; Ricci, Ritchelli ; Marciano, Fernanda Roberta ; Prokofiev, Egor ; Valiev, Ruslan Z. ; Lobo, Anderson Oliveira ; Jorge Júnior, Alberto Moreira. / Biological response of chemically treated surface of the ultrafine-grained Ti-6Al-7Nb alloy for biomedical applications. В: International Journal of Nanomedicine. 2019 ; Том 14. стр. 1725-1736.
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title = "Biological response of chemically treated surface of the ultrafine-grained Ti-6Al-7Nb alloy for biomedical applications",
abstract = "Background: Nanophase surface properties of titanium alloys must be obtained for a suitable biological performance, particularly to facilitate cell adhesion and bone tissue formation. Obtaining a bulk nanostructured material using severe plastic deformation is an ideal processing route to improve the mechanical performance of titanium alloys. By decreasing the grain size of a metallic material, a superior strength improvement can be obtained, while surface modification of a nanostructured surface can produce an attractive topography able to induce biological responses in osteoblastic cells. Methods: Aiming to achieve such an excellent synergetic performance, a processing route, which included equal channel angular pressing (ECAP), hot and cold extrusion, and heat treatments, was used to produce a nanometric and ultrafine-grained (UFG) microstructure in the Ti-6Al-7Nb alloy (around of 200 nm). Additionally, UFG samples were surface-modified with acid etching (UFG-A) to produce a uniform micron and submicron porosity on the surface. Subsequently, alkaline treatment (UFG-AA) produced a sponge-like nanotopographic substrate able to modulate cellular interactions. Results: After several kinds of biological tests for both treatment conditions (UFG-A and UFG-AA), the main results have shown that there was no cytotoxicity, expressed alkaline phosphatase activity and total protein amounts without statistical differences compared to control. However, the UFG-AA samples presented an attractive effect on the cell membranes, and cell adhesions were preferentially induced as compared with UFG-A. Both conditions demonstrated cell projections, but for UFG-AA, cells were more widely dispersed, and more quantities of filopodia formation could be observed. Conclusion: Herein, the reasons for such behaviors are discussed, and further results are presented in addition to those mentioned above.",
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author = "{de Oliveira}, {Diego Pedreira} and Toniato, {Tatiane Venturott} and Ritchelli Ricci and Marciano, {Fernanda Roberta} and Egor Prokofiev and Valiev, {Ruslan Z.} and Lobo, {Anderson Oliveira} and {Jorge J{\'u}nior}, {Alberto Moreira}",
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Biological response of chemically treated surface of the ultrafine-grained Ti-6Al-7Nb alloy for biomedical applications. / de Oliveira, Diego Pedreira; Toniato, Tatiane Venturott; Ricci, Ritchelli; Marciano, Fernanda Roberta; Prokofiev, Egor; Valiev, Ruslan Z.; Lobo, Anderson Oliveira; Jorge Júnior, Alberto Moreira.

В: International Journal of Nanomedicine, Том 14, 01.01.2019, стр. 1725-1736.

Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

TY - JOUR

T1 - Biological response of chemically treated surface of the ultrafine-grained Ti-6Al-7Nb alloy for biomedical applications

AU - de Oliveira, Diego Pedreira

AU - Toniato, Tatiane Venturott

AU - Ricci, Ritchelli

AU - Marciano, Fernanda Roberta

AU - Prokofiev, Egor

AU - Valiev, Ruslan Z.

AU - Lobo, Anderson Oliveira

AU - Jorge Júnior, Alberto Moreira

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: Nanophase surface properties of titanium alloys must be obtained for a suitable biological performance, particularly to facilitate cell adhesion and bone tissue formation. Obtaining a bulk nanostructured material using severe plastic deformation is an ideal processing route to improve the mechanical performance of titanium alloys. By decreasing the grain size of a metallic material, a superior strength improvement can be obtained, while surface modification of a nanostructured surface can produce an attractive topography able to induce biological responses in osteoblastic cells. Methods: Aiming to achieve such an excellent synergetic performance, a processing route, which included equal channel angular pressing (ECAP), hot and cold extrusion, and heat treatments, was used to produce a nanometric and ultrafine-grained (UFG) microstructure in the Ti-6Al-7Nb alloy (around of 200 nm). Additionally, UFG samples were surface-modified with acid etching (UFG-A) to produce a uniform micron and submicron porosity on the surface. Subsequently, alkaline treatment (UFG-AA) produced a sponge-like nanotopographic substrate able to modulate cellular interactions. Results: After several kinds of biological tests for both treatment conditions (UFG-A and UFG-AA), the main results have shown that there was no cytotoxicity, expressed alkaline phosphatase activity and total protein amounts without statistical differences compared to control. However, the UFG-AA samples presented an attractive effect on the cell membranes, and cell adhesions were preferentially induced as compared with UFG-A. Both conditions demonstrated cell projections, but for UFG-AA, cells were more widely dispersed, and more quantities of filopodia formation could be observed. Conclusion: Herein, the reasons for such behaviors are discussed, and further results are presented in addition to those mentioned above.

AB - Background: Nanophase surface properties of titanium alloys must be obtained for a suitable biological performance, particularly to facilitate cell adhesion and bone tissue formation. Obtaining a bulk nanostructured material using severe plastic deformation is an ideal processing route to improve the mechanical performance of titanium alloys. By decreasing the grain size of a metallic material, a superior strength improvement can be obtained, while surface modification of a nanostructured surface can produce an attractive topography able to induce biological responses in osteoblastic cells. Methods: Aiming to achieve such an excellent synergetic performance, a processing route, which included equal channel angular pressing (ECAP), hot and cold extrusion, and heat treatments, was used to produce a nanometric and ultrafine-grained (UFG) microstructure in the Ti-6Al-7Nb alloy (around of 200 nm). Additionally, UFG samples were surface-modified with acid etching (UFG-A) to produce a uniform micron and submicron porosity on the surface. Subsequently, alkaline treatment (UFG-AA) produced a sponge-like nanotopographic substrate able to modulate cellular interactions. Results: After several kinds of biological tests for both treatment conditions (UFG-A and UFG-AA), the main results have shown that there was no cytotoxicity, expressed alkaline phosphatase activity and total protein amounts without statistical differences compared to control. However, the UFG-AA samples presented an attractive effect on the cell membranes, and cell adhesions were preferentially induced as compared with UFG-A. Both conditions demonstrated cell projections, but for UFG-AA, cells were more widely dispersed, and more quantities of filopodia formation could be observed. Conclusion: Herein, the reasons for such behaviors are discussed, and further results are presented in addition to those mentioned above.

KW - biological response

KW - ECAP

KW - implants

KW - SPD

KW - surface treatment

KW - UFG Ti–6Al–7Nb alloy

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DO - 10.2147/IJN.S197099

M3 - Article

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JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

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