Autophosphorylation of MAP kinase disables the MAPK pathway in apoptotic Xenopus eggs

A. Tokmakov, Kousuke Akino, Sho Iguchi, Tetsushi Iwasaki, Vasily E. Stefanov, Ken-Ichi Sato

Результат исследований: Научные публикации в периодических изданияхстатья

Выдержка

Mitogen-activated protein kinases (MAPKs) are involved in the regulation of various cellular processes, including cell survival and apoptosis. Here, we report that Xenopus p42 MAPK becomes phosphorylated in apoptotic eggs, however this modification does not activate the enzyme. Using phosphorylation residue-specific antibodies, we demonstrate that this modification occurs on the Tyr residue in the MAPK activation segment, pinpointing the autophosphorylation mechanism. Notably, MAPK phosphorylation in apoptotic Xenopus eggs coincides with prominent intracellular acidification accompanying apoptosis in these cells. Furthermore, autophosphorylation of recombinant Xenopus MAPK is stimulated and phosphorylation of a protein substrate is inhibited under low pH conditions. Thus, acidic intracellular conditions inactivate MAPK and effectively disable the MAPK-mediated survival pathway in the apoptotic eggs. Given that cell acidification is a rather common feature of apoptosis, we hypothesize that stimulation of MAPK autophosphorylation and shutdown of the MAPK pathway may represent universal traits of apoptotic cell death.

Язык оригиналаанглийский
Страницы (с-по)140-145
ЖурналBiochemical and Biophysical Research Communications
Том517
Номер выпуска1
Ранняя дата в режиме онлайн16 июл 2019
DOI
СостояниеОпубликовано - сен 2019

Отпечаток

Xenopus
Mitogen-Activated Protein Kinases
Eggs
Phosphotransferases
Phosphorylation
Acidification
Apoptosis
Mitogen-Activated Protein Kinase 1
Cell death
Cell Survival
Cell Death
Chemical activation
Cells
Antibodies
Substrates
Enzymes

Предметные области Scopus

  • Биофизика
  • Биохимия
  • Молекулярная биология
  • Клеточная биология

Цитировать

Tokmakov, A. ; Akino, Kousuke ; Iguchi, Sho ; Iwasaki, Tetsushi ; Stefanov, Vasily E. ; Sato, Ken-Ichi. / Autophosphorylation of MAP kinase disables the MAPK pathway in apoptotic Xenopus eggs. В: Biochemical and Biophysical Research Communications. 2019 ; Том 517, № 1. стр. 140-145.
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title = "Autophosphorylation of MAP kinase disables the MAPK pathway in apoptotic Xenopus eggs",
abstract = "Mitogen-activated protein kinases (MAPKs) are involved in the regulation of various cellular processes, including cell survival and apoptosis. Here, we report that Xenopus p42 MAPK becomes phosphorylated in apoptotic eggs, however this modification does not activate the enzyme. Using phosphorylation residue-specific antibodies, we demonstrate that this modification occurs on the Tyr residue in the MAPK activation segment, pinpointing the autophosphorylation mechanism. Notably, MAPK phosphorylation in apoptotic Xenopus eggs coincides with prominent intracellular acidification accompanying apoptosis in these cells. Furthermore, autophosphorylation of recombinant Xenopus MAPK is stimulated and phosphorylation of a protein substrate is inhibited under low pH conditions. Thus, acidic intracellular conditions inactivate MAPK and effectively disable the MAPK-mediated survival pathway in the apoptotic eggs. Given that cell acidification is a rather common feature of apoptosis, we hypothesize that stimulation of MAPK autophosphorylation and shutdown of the MAPK pathway may represent universal traits of apoptotic cell death.",
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Autophosphorylation of MAP kinase disables the MAPK pathway in apoptotic Xenopus eggs. / Tokmakov, A.; Akino, Kousuke; Iguchi, Sho; Iwasaki, Tetsushi; Stefanov, Vasily E.; Sato, Ken-Ichi.

В: Biochemical and Biophysical Research Communications, Том 517, № 1, 09.2019, стр. 140-145.

Результат исследований: Научные публикации в периодических изданияхстатья

TY - JOUR

T1 - Autophosphorylation of MAP kinase disables the MAPK pathway in apoptotic Xenopus eggs

AU - Tokmakov, A.

AU - Akino, Kousuke

AU - Iguchi, Sho

AU - Iwasaki, Tetsushi

AU - Stefanov, Vasily E.

AU - Sato, Ken-Ichi

PY - 2019/9

Y1 - 2019/9

N2 - Mitogen-activated protein kinases (MAPKs) are involved in the regulation of various cellular processes, including cell survival and apoptosis. Here, we report that Xenopus p42 MAPK becomes phosphorylated in apoptotic eggs, however this modification does not activate the enzyme. Using phosphorylation residue-specific antibodies, we demonstrate that this modification occurs on the Tyr residue in the MAPK activation segment, pinpointing the autophosphorylation mechanism. Notably, MAPK phosphorylation in apoptotic Xenopus eggs coincides with prominent intracellular acidification accompanying apoptosis in these cells. Furthermore, autophosphorylation of recombinant Xenopus MAPK is stimulated and phosphorylation of a protein substrate is inhibited under low pH conditions. Thus, acidic intracellular conditions inactivate MAPK and effectively disable the MAPK-mediated survival pathway in the apoptotic eggs. Given that cell acidification is a rather common feature of apoptosis, we hypothesize that stimulation of MAPK autophosphorylation and shutdown of the MAPK pathway may represent universal traits of apoptotic cell death.

AB - Mitogen-activated protein kinases (MAPKs) are involved in the regulation of various cellular processes, including cell survival and apoptosis. Here, we report that Xenopus p42 MAPK becomes phosphorylated in apoptotic eggs, however this modification does not activate the enzyme. Using phosphorylation residue-specific antibodies, we demonstrate that this modification occurs on the Tyr residue in the MAPK activation segment, pinpointing the autophosphorylation mechanism. Notably, MAPK phosphorylation in apoptotic Xenopus eggs coincides with prominent intracellular acidification accompanying apoptosis in these cells. Furthermore, autophosphorylation of recombinant Xenopus MAPK is stimulated and phosphorylation of a protein substrate is inhibited under low pH conditions. Thus, acidic intracellular conditions inactivate MAPK and effectively disable the MAPK-mediated survival pathway in the apoptotic eggs. Given that cell acidification is a rather common feature of apoptosis, we hypothesize that stimulation of MAPK autophosphorylation and shutdown of the MAPK pathway may represent universal traits of apoptotic cell death.

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