An ultrastructural and molecular study of Hyperamoeba dachnaya, n. sp., and its relationship to the mycetozoan slime moulds

Giselle Walker, Jeffrey D. Silberman, Serguei A. Karpov, Angelika Preisfeld, Peter Foster, Alexander O. Frolov, Yuri Novozhilov, Mitchell L. Sogin

Результат исследований: Научные публикации в периодических изданияхстатья

26 Цитирования (Scopus)

Выдержка

Hyperamoeba dachnaya, a new taxon affiliated with Hyperamoeba flagellata, is described from freshwater anaerobic sediments. Organisms exist as amoeboid flagellates, amoebae and cysts. The flagellates of H. dachnaya are distinguished from those of H. flagellata by larger size and a longer anteriorly-directed finger-shaped pseudopodium. H. dachnaya cysts have bilaminar walls with connectives between the outer and inner layers appearing to be T-shaped spines, while cyst walls of H. flagellata are smooth. H. dachnaya has two basal bodies and a single anteriorly-directed flagellum emerging from the anterior one (BB1). A fibrillar rootlet with a microtubule organising centre (MTOC;'R1') arises from a diaphragm-shaped basal plate at the base of BB1; a short striated fibre (SSF) covers the dorsal side of BB1 and initiates microtubular rootlets 'R2' and 'R3' and the fibrillar bridge leading to microtubular rootlet 'R4'. 'R5' is composed of 2 microtubules splitting from R4 and 2 others initiating by BB1. The posterior basal body is associated with a fibrillar posterior parakinetosomal structure. A long striated fibre arises in association with the right end of the SSF. A Golgi apparatus is associated with the flagellar apparatus.The flagellar apparatus is present internally in amoebae. All three stages have mitochondria with tubular cristae and an electron-dense central body. Analysis of small subunit ribosomal RNA sequences shows H. dachnaya branching with the myxogastrids within the mycetozoa, but polyphyletic with a nominal Hyperamoeba sp. isolate that is morphologically similar to H. flagellata. We reject the suggestion that H. flagellata and this Hyperamoeba sp. isolate of Zaman et al. (1999) are conspecific. The study reveals extensive similarities between Hyperamoeba and the flagellated stages of myxogastrids.

Язык оригиналаанглийский
Страницы (с-по)319-336
Число страниц18
ЖурналEuropean Journal of Protistology
Том39
Номер выпуска3
DOI
СостояниеОпубликовано - 1 янв 2003

Отпечаток

Microtubule-Organizing Center
Basal Bodies
Cysts
Amoeba
Fungi
Mycetozoa
Pseudopodia
Ribosomal RNA
Flagella
Golgi Apparatus
Diaphragm
Fresh Water
Microtubules
Fingers
Mitochondria
Spine
Electrons

Предметные области Scopus

  • Микробиология

Цитировать

Walker, Giselle ; Silberman, Jeffrey D. ; Karpov, Serguei A. ; Preisfeld, Angelika ; Foster, Peter ; Frolov, Alexander O. ; Novozhilov, Yuri ; Sogin, Mitchell L. / An ultrastructural and molecular study of Hyperamoeba dachnaya, n. sp., and its relationship to the mycetozoan slime moulds. В: European Journal of Protistology. 2003 ; Том 39, № 3. стр. 319-336.
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abstract = "Hyperamoeba dachnaya, a new taxon affiliated with Hyperamoeba flagellata, is described from freshwater anaerobic sediments. Organisms exist as amoeboid flagellates, amoebae and cysts. The flagellates of H. dachnaya are distinguished from those of H. flagellata by larger size and a longer anteriorly-directed finger-shaped pseudopodium. H. dachnaya cysts have bilaminar walls with connectives between the outer and inner layers appearing to be T-shaped spines, while cyst walls of H. flagellata are smooth. H. dachnaya has two basal bodies and a single anteriorly-directed flagellum emerging from the anterior one (BB1). A fibrillar rootlet with a microtubule organising centre (MTOC;'R1') arises from a diaphragm-shaped basal plate at the base of BB1; a short striated fibre (SSF) covers the dorsal side of BB1 and initiates microtubular rootlets 'R2' and 'R3' and the fibrillar bridge leading to microtubular rootlet 'R4'. 'R5' is composed of 2 microtubules splitting from R4 and 2 others initiating by BB1. The posterior basal body is associated with a fibrillar posterior parakinetosomal structure. A long striated fibre arises in association with the right end of the SSF. A Golgi apparatus is associated with the flagellar apparatus.The flagellar apparatus is present internally in amoebae. All three stages have mitochondria with tubular cristae and an electron-dense central body. Analysis of small subunit ribosomal RNA sequences shows H. dachnaya branching with the myxogastrids within the mycetozoa, but polyphyletic with a nominal Hyperamoeba sp. isolate that is morphologically similar to H. flagellata. We reject the suggestion that H. flagellata and this Hyperamoeba sp. isolate of Zaman et al. (1999) are conspecific. The study reveals extensive similarities between Hyperamoeba and the flagellated stages of myxogastrids.",
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An ultrastructural and molecular study of Hyperamoeba dachnaya, n. sp., and its relationship to the mycetozoan slime moulds. / Walker, Giselle; Silberman, Jeffrey D.; Karpov, Serguei A.; Preisfeld, Angelika; Foster, Peter; Frolov, Alexander O.; Novozhilov, Yuri; Sogin, Mitchell L.

В: European Journal of Protistology, Том 39, № 3, 01.01.2003, стр. 319-336.

Результат исследований: Научные публикации в периодических изданияхстатья

TY - JOUR

T1 - An ultrastructural and molecular study of Hyperamoeba dachnaya, n. sp., and its relationship to the mycetozoan slime moulds

AU - Walker, Giselle

AU - Silberman, Jeffrey D.

AU - Karpov, Serguei A.

AU - Preisfeld, Angelika

AU - Foster, Peter

AU - Frolov, Alexander O.

AU - Novozhilov, Yuri

AU - Sogin, Mitchell L.

PY - 2003/1/1

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N2 - Hyperamoeba dachnaya, a new taxon affiliated with Hyperamoeba flagellata, is described from freshwater anaerobic sediments. Organisms exist as amoeboid flagellates, amoebae and cysts. The flagellates of H. dachnaya are distinguished from those of H. flagellata by larger size and a longer anteriorly-directed finger-shaped pseudopodium. H. dachnaya cysts have bilaminar walls with connectives between the outer and inner layers appearing to be T-shaped spines, while cyst walls of H. flagellata are smooth. H. dachnaya has two basal bodies and a single anteriorly-directed flagellum emerging from the anterior one (BB1). A fibrillar rootlet with a microtubule organising centre (MTOC;'R1') arises from a diaphragm-shaped basal plate at the base of BB1; a short striated fibre (SSF) covers the dorsal side of BB1 and initiates microtubular rootlets 'R2' and 'R3' and the fibrillar bridge leading to microtubular rootlet 'R4'. 'R5' is composed of 2 microtubules splitting from R4 and 2 others initiating by BB1. The posterior basal body is associated with a fibrillar posterior parakinetosomal structure. A long striated fibre arises in association with the right end of the SSF. A Golgi apparatus is associated with the flagellar apparatus.The flagellar apparatus is present internally in amoebae. All three stages have mitochondria with tubular cristae and an electron-dense central body. Analysis of small subunit ribosomal RNA sequences shows H. dachnaya branching with the myxogastrids within the mycetozoa, but polyphyletic with a nominal Hyperamoeba sp. isolate that is morphologically similar to H. flagellata. We reject the suggestion that H. flagellata and this Hyperamoeba sp. isolate of Zaman et al. (1999) are conspecific. The study reveals extensive similarities between Hyperamoeba and the flagellated stages of myxogastrids.

AB - Hyperamoeba dachnaya, a new taxon affiliated with Hyperamoeba flagellata, is described from freshwater anaerobic sediments. Organisms exist as amoeboid flagellates, amoebae and cysts. The flagellates of H. dachnaya are distinguished from those of H. flagellata by larger size and a longer anteriorly-directed finger-shaped pseudopodium. H. dachnaya cysts have bilaminar walls with connectives between the outer and inner layers appearing to be T-shaped spines, while cyst walls of H. flagellata are smooth. H. dachnaya has two basal bodies and a single anteriorly-directed flagellum emerging from the anterior one (BB1). A fibrillar rootlet with a microtubule organising centre (MTOC;'R1') arises from a diaphragm-shaped basal plate at the base of BB1; a short striated fibre (SSF) covers the dorsal side of BB1 and initiates microtubular rootlets 'R2' and 'R3' and the fibrillar bridge leading to microtubular rootlet 'R4'. 'R5' is composed of 2 microtubules splitting from R4 and 2 others initiating by BB1. The posterior basal body is associated with a fibrillar posterior parakinetosomal structure. A long striated fibre arises in association with the right end of the SSF. A Golgi apparatus is associated with the flagellar apparatus.The flagellar apparatus is present internally in amoebae. All three stages have mitochondria with tubular cristae and an electron-dense central body. Analysis of small subunit ribosomal RNA sequences shows H. dachnaya branching with the myxogastrids within the mycetozoa, but polyphyletic with a nominal Hyperamoeba sp. isolate that is morphologically similar to H. flagellata. We reject the suggestion that H. flagellata and this Hyperamoeba sp. isolate of Zaman et al. (1999) are conspecific. The study reveals extensive similarities between Hyperamoeba and the flagellated stages of myxogastrids.

KW - Classification

KW - Hyperamoeba dachnaya n. sp.

KW - Myxogastrid

KW - Protist

KW - Protozoa

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U2 - 10.1078/0932-4739-00906

DO - 10.1078/0932-4739-00906

M3 - Article

AN - SCOPUS:0141993555

VL - 39

SP - 319

EP - 336

JO - European Journal of Protistology

JF - European Journal of Protistology

SN - 0932-4739

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