A new method for vibration-based neurophenotyping of zebrafish

Jingtao Wang, Dongmei Wang, Guojun Hu, LongEn Yang, Dongni Yan, Mengyao Wang, Nazar Serikuly, Erik Alpyshov, Tamara G Amstislavskaya, Konstantin A Demin, Murilo S de Abreu, Konstantin N Zabegalov, Allan V Kalueff

Результат исследований: Научные публикации в периодических изданияхстатья

2 Цитирования (Scopus)

Аннотация

The zebrafish (Danio rerio) is rapidly emerging as an important model species in neuroscience research. Neurobehavioral studies in zebrafish are typically based on automated video-tracking of individual or group fish responses to various stressors, drug treatments and genetic manipulations. However, moving zebrafish also emit vibration signals that can be recorded and characterized. New method: Here, we present the first evidence that vibration-based analyses can be used to assess zebrafish behaviors. Utilizing a free accelerometer smartphone application, we developed a simple inexpensive custom-made setup to detect vibration signals in adult zebrafish. Results: We demonstrate that moving zebrafish generate detectable, reproducible vibration power frequency spectra that may be sensitive to various experimental manipulations, including sedative and anxiolytic treatments. Comparison with existing methods: The present study is the first report describing vibration-based behavioral characterization in zebrafish. Conclusions: The present proof-of-concept study expands the toolkit of zebrafish neurophenotyping methods to include vibration data, which may not only reflect major global changes in zebrafish locomotion (e.g., sedation or hyperactivity), but can also eventually help detect more nuanced, behavior- or context-specific changes in zebrafish phenotypes.

Язык оригиналаанглийский
Номер статьи108563
ЖурналJournal of Neuroscience Methods
Том333
Ранняя дата в режиме онлайн18 дек 2019
DOI
СостояниеОпубликовано - 1 мар 2020

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Предметные области Scopus

  • Нейробиология (все)

Цитировать

Wang, J., Wang, D., Hu, G., Yang, L., Yan, D., Wang, M., ... Kalueff, A. V. (2020). A new method for vibration-based neurophenotyping of zebrafish. Journal of Neuroscience Methods, 333, [108563]. https://doi.org/10.1016/j.jneumeth.2019.108563