Exogenous ascorbate as a signalling molecule in plants

Переведенное название: Экзогенная аскорбиновая кислота как сигнальная молекула в растениях

Вадим Викторович Демидчик, M. Vaitsiakhovich, D. Svistunenko, I. Navaselsky, P. Hryvusevich, V. Mackievic, V. Samokhina, Григорий Александрович Пожванов, D. Straltsova, Галина Николаевна Смоликова, Сергей Семенович Медведев, A. Sokolik

Результат исследований: Материалы конференцийтезисынаучная

Выдержка

Plant cell signaling relies on a multitude of primary and secondary messenger molecules. Exogenous L-ascorbic acid (ascorbate) has not been considered as a signaling molecule in plant cells. However we have shown that, in Arabidopsis thaliana L. root cells, exogenous ascorbate (>30 μM) induces a transient increase of the cytosolic free Ca2+ activity ([Ca2+]cyt.). This phenomenon is fundamental to plant signaling because it underlies transmission of most important plant signals, such as hormones and stress. Exogenous copper and iron stimulated the ascorbate-induced [Ca2+]cyt. elevation while cation channel blockers, free radical scavengers, low extracellular [Ca2+], transition metal chelators and removal of the cell wall inhibited this reaction. These data show that the apoplastic redox- active transition metals are involved in the ascorbate-induced [Ca2+]cyt. elevation. Exogenous ascorbate also induced moderate increase in programmed cell death symptoms in intact roots, but it did not activate Ca2+ influx currents in patch-clamped root protoplasts. Intriguingly, replacement of gluconate with ascorbate in the patch-clamp pipette revealed a large ascorbate efflux current, which showed sensitivity to anion channel blocker, anthracene-9-carboxylic acid (A9C), indicative of the ascorbate release via anion channels. EPR spectroscopy measurements demonstrated that salinity (NaCl) triggered accumulation of root apoplastic ascorbyl radicals in A9C-dependent manner, confirming that L-ascorbate leaks through anion channels under depolarisation. This mechanism can underlie ascorbate release, signaling phenomena, apoplastic redox reactions, iron acquisition and control of membrane ionic and electrical equilibrium (together K+ efflux via GORK channels). Financial support of the Russian Science Foundation (grant#15-14-30008 to VD) is gratefully acknowledged.
Язык оригиналаанглийский
Страницы46
Число страниц1
СостояниеОпубликовано - мая 2018
СобытиеКлеточная биология и биотехнология растений: Школа молодых ученых "Биология растительной клетки: от теории к практике - Белорусский Государственный Университет. Институт Леса НАН Беларуси, Минск, Белоруссия
Продолжительность: 28 мая 201831 окт 2018
http://www.conf.bsu.by/pcbb2018

Конференция

КонференцияКлеточная биология и биотехнология растений
СтранаБелоруссия
ГородМинск
Период28/05/1831/10/18
Адрес в сети Интернет

Цитировать

Демидчик, В. В., Vaitsiakhovich, M., Svistunenko, D., Navaselsky, I., Hryvusevich, P., Mackievic, V., ... Sokolik, A. (2018). Exogenous ascorbate as a signalling molecule in plants. 46. Выдержка из Клеточная биология и биотехнология растений, Минск, Белоруссия.
Демидчик, Вадим Викторович ; Vaitsiakhovich, M. ; Svistunenko, D. ; Navaselsky, I. ; Hryvusevich, P. ; Mackievic, V. ; Samokhina, V. ; Пожванов, Григорий Александрович ; Straltsova, D. ; Смоликова, Галина Николаевна ; Медведев, Сергей Семенович ; Sokolik, A. / Exogenous ascorbate as a signalling molecule in plants. Выдержка из Клеточная биология и биотехнология растений, Минск, Белоруссия.1 стр.
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title = "Exogenous ascorbate as a signalling molecule in plants",
abstract = "Plant cell signaling relies on a multitude of primary and secondary messenger molecules. Exogenous L-ascorbic acid (ascorbate) has not been considered as a signaling molecule in plant cells. However we have shown that, in Arabidopsis thaliana L. root cells, exogenous ascorbate (>30 μM) induces a transient increase of the cytosolic free Ca2+ activity ([Ca2+]cyt.). This phenomenon is fundamental to plant signaling because it underlies transmission of most important plant signals, such as hormones and stress. Exogenous copper and iron stimulated the ascorbate-induced [Ca2+]cyt. elevation while cation channel blockers, free radical scavengers, low extracellular [Ca2+], transition metal chelators and removal of the cell wall inhibited this reaction. These data show that the apoplastic redox- active transition metals are involved in the ascorbate-induced [Ca2+]cyt. elevation. Exogenous ascorbate also induced moderate increase in programmed cell death symptoms in intact roots, but it did not activate Ca2+ influx currents in patch-clamped root protoplasts. Intriguingly, replacement of gluconate with ascorbate in the patch-clamp pipette revealed a large ascorbate efflux current, which showed sensitivity to anion channel blocker, anthracene-9-carboxylic acid (A9C), indicative of the ascorbate release via anion channels. EPR spectroscopy measurements demonstrated that salinity (NaCl) triggered accumulation of root apoplastic ascorbyl radicals in A9C-dependent manner, confirming that L-ascorbate leaks through anion channels under depolarisation. This mechanism can underlie ascorbate release, signaling phenomena, apoplastic redox reactions, iron acquisition and control of membrane ionic and electrical equilibrium (together K+ efflux via GORK channels). Financial support of the Russian Science Foundation (grant#15-14-30008 to VD) is gratefully acknowledged.",
author = "Демидчик, {Вадим Викторович} and M. Vaitsiakhovich and D. Svistunenko and I. Navaselsky and P. Hryvusevich and V. Mackievic and V. Samokhina and Пожванов, {Григорий Александрович} and D. Straltsova and Смоликова, {Галина Николаевна} and Медведев, {Сергей Семенович} and A. Sokolik",
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note = "null ; Conference date: 28-05-2018 Through 31-10-2018",
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Демидчик, ВВ, Vaitsiakhovich, M, Svistunenko, D, Navaselsky, I, Hryvusevich, P, Mackievic, V, Samokhina, V, Пожванов, ГА, Straltsova, D, Смоликова, ГН, Медведев, СС & Sokolik, A 2018, 'Exogenous ascorbate as a signalling molecule in plants' Клеточная биология и биотехнология растений, Минск, Белоруссия, 28/05/18 - 31/10/18, стр. 46.

Exogenous ascorbate as a signalling molecule in plants. / Демидчик, Вадим Викторович; Vaitsiakhovich, M.; Svistunenko, D.; Navaselsky, I.; Hryvusevich, P.; Mackievic, V.; Samokhina, V.; Пожванов, Григорий Александрович; Straltsova, D.; Смоликова, Галина Николаевна; Медведев, Сергей Семенович; Sokolik, A.

2018. 46 Выдержка из Клеточная биология и биотехнология растений, Минск, Белоруссия.

Результат исследований: Материалы конференцийтезисынаучная

TY - CONF

T1 - Exogenous ascorbate as a signalling molecule in plants

AU - Демидчик, Вадим Викторович

AU - Vaitsiakhovich, M.

AU - Svistunenko, D.

AU - Navaselsky, I.

AU - Hryvusevich, P.

AU - Mackievic, V.

AU - Samokhina, V.

AU - Пожванов, Григорий Александрович

AU - Straltsova, D.

AU - Смоликова, Галина Николаевна

AU - Медведев, Сергей Семенович

AU - Sokolik, A.

PY - 2018/5

Y1 - 2018/5

N2 - Plant cell signaling relies on a multitude of primary and secondary messenger molecules. Exogenous L-ascorbic acid (ascorbate) has not been considered as a signaling molecule in plant cells. However we have shown that, in Arabidopsis thaliana L. root cells, exogenous ascorbate (>30 μM) induces a transient increase of the cytosolic free Ca2+ activity ([Ca2+]cyt.). This phenomenon is fundamental to plant signaling because it underlies transmission of most important plant signals, such as hormones and stress. Exogenous copper and iron stimulated the ascorbate-induced [Ca2+]cyt. elevation while cation channel blockers, free radical scavengers, low extracellular [Ca2+], transition metal chelators and removal of the cell wall inhibited this reaction. These data show that the apoplastic redox- active transition metals are involved in the ascorbate-induced [Ca2+]cyt. elevation. Exogenous ascorbate also induced moderate increase in programmed cell death symptoms in intact roots, but it did not activate Ca2+ influx currents in patch-clamped root protoplasts. Intriguingly, replacement of gluconate with ascorbate in the patch-clamp pipette revealed a large ascorbate efflux current, which showed sensitivity to anion channel blocker, anthracene-9-carboxylic acid (A9C), indicative of the ascorbate release via anion channels. EPR spectroscopy measurements demonstrated that salinity (NaCl) triggered accumulation of root apoplastic ascorbyl radicals in A9C-dependent manner, confirming that L-ascorbate leaks through anion channels under depolarisation. This mechanism can underlie ascorbate release, signaling phenomena, apoplastic redox reactions, iron acquisition and control of membrane ionic and electrical equilibrium (together K+ efflux via GORK channels). Financial support of the Russian Science Foundation (grant#15-14-30008 to VD) is gratefully acknowledged.

AB - Plant cell signaling relies on a multitude of primary and secondary messenger molecules. Exogenous L-ascorbic acid (ascorbate) has not been considered as a signaling molecule in plant cells. However we have shown that, in Arabidopsis thaliana L. root cells, exogenous ascorbate (>30 μM) induces a transient increase of the cytosolic free Ca2+ activity ([Ca2+]cyt.). This phenomenon is fundamental to plant signaling because it underlies transmission of most important plant signals, such as hormones and stress. Exogenous copper and iron stimulated the ascorbate-induced [Ca2+]cyt. elevation while cation channel blockers, free radical scavengers, low extracellular [Ca2+], transition metal chelators and removal of the cell wall inhibited this reaction. These data show that the apoplastic redox- active transition metals are involved in the ascorbate-induced [Ca2+]cyt. elevation. Exogenous ascorbate also induced moderate increase in programmed cell death symptoms in intact roots, but it did not activate Ca2+ influx currents in patch-clamped root protoplasts. Intriguingly, replacement of gluconate with ascorbate in the patch-clamp pipette revealed a large ascorbate efflux current, which showed sensitivity to anion channel blocker, anthracene-9-carboxylic acid (A9C), indicative of the ascorbate release via anion channels. EPR spectroscopy measurements demonstrated that salinity (NaCl) triggered accumulation of root apoplastic ascorbyl radicals in A9C-dependent manner, confirming that L-ascorbate leaks through anion channels under depolarisation. This mechanism can underlie ascorbate release, signaling phenomena, apoplastic redox reactions, iron acquisition and control of membrane ionic and electrical equilibrium (together K+ efflux via GORK channels). Financial support of the Russian Science Foundation (grant#15-14-30008 to VD) is gratefully acknowledged.

M3 - Abstract

SP - 46

ER -

Демидчик ВВ, Vaitsiakhovich M, Svistunenko D, Navaselsky I, Hryvusevich P, Mackievic V и соавт.. Exogenous ascorbate as a signalling molecule in plants. 2018. Выдержка из Клеточная биология и биотехнология растений, Минск, Белоруссия.