Prospects for the Application of Yeast Display in Biotechnology and Cell Biology (Review)

Переведенное название: ПЕРСПЕКТИВЫ ИСПОЛЬЗОВАНИЯ ДРОЖЖЕВОГО ДИСПЛЕЯ В БИОТЕХНОЛОГИИ И КЛЕТОЧНОЙ БИОЛОГИИ

Результат исследований: Научные публикации в периодических изданияхОбзорная статья

2 Цитирования (Scopus)

Выдержка

The technology of the yeast cell surface display, which appeared 20 years ago and was based on the displaying of target proteins on the cell surface via fusion to an abundant cell wall protein finds broad application in basic and applied research. The main advantage of the cell surface display on the basis of eukaryotic microorganisms—yeast—is the opportunity for correct modification of mammalian proteins. The cell surface display is an important tool for the analysis and understanding of protein function and protein–protein interactions and for the screening of novel clones from peptide and protein libraries. This technology makes it possible to obtain cells with novel abilities, such as catalytic functions and affinity binding to valuable ligands, including rare and heavy metals. It provides the chance to use yeast in biotechnology and in bioremediation and biomonitoring of the environment. The review considers the methods of obtaining a cell surface display on the basis of the yeasts Saccharomyces cerevisiae, Pichia pastoris, and Yarrowia lipolytica, the properties of anchor proteins, and the main fields of yeast display technology.

Язык оригиналаанглийский
Страницы (с-по)337-351
Число страниц15
ЖурналApplied Biochemistry and Microbiology
Том54
Номер выпуска4
DOI
СостояниеОпубликовано - 1 июл 2018

Отпечаток

Cytology
Biotechnology
Yeast
Cell Biology
Yeasts
Display devices
Proteins
Cell Surface Display Techniques
Yarrowia
Technology
Peptide Library
Cells
Environmental Biodegradation
Pichia
Environmental Monitoring
Heavy Metals
Bioremediation
Cell Wall
Saccharomyces cerevisiae
Anchors

Предметные области Scopus

  • Биохимия, генетика и молекулярная биология (все)
  • Прикладная микробиология и биотехника
  • Биохимия

Ключевые слова

  • yeast surface display, anchor proteins, immobilized enzymes stability, metal recovery, bioremediation, biomonitoring

Цитировать

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abstract = "The technology of the yeast cell surface display, which appeared 20 years ago and was based on the displaying of target proteins on the cell surface via fusion to an abundant cell wall protein finds broad application in basic and applied research. The main advantage of the cell surface display on the basis of eukaryotic microorganisms—yeast—is the opportunity for correct modification of mammalian proteins. The cell surface display is an important tool for the analysis and understanding of protein function and protein–protein interactions and for the screening of novel clones from peptide and protein libraries. This technology makes it possible to obtain cells with novel abilities, such as catalytic functions and affinity binding to valuable ligands, including rare and heavy metals. It provides the chance to use yeast in biotechnology and in bioremediation and biomonitoring of the environment. The review considers the methods of obtaining a cell surface display on the basis of the yeasts Saccharomyces cerevisiae, Pichia pastoris, and Yarrowia lipolytica, the properties of anchor proteins, and the main fields of yeast display technology.",
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author = "M.V. Padkina and E.V. Sambuk",
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Prospects for the Application of Yeast Display in Biotechnology and Cell Biology (Review). / Padkina, M.V.; Sambuk, E.V.

В: Applied Biochemistry and Microbiology, Том 54, № 4, 01.07.2018, стр. 337-351.

Результат исследований: Научные публикации в периодических изданияхОбзорная статья

TY - JOUR

T1 - Prospects for the Application of Yeast Display in Biotechnology and Cell Biology (Review)

AU - Padkina, M.V.

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AB - The technology of the yeast cell surface display, which appeared 20 years ago and was based on the displaying of target proteins on the cell surface via fusion to an abundant cell wall protein finds broad application in basic and applied research. The main advantage of the cell surface display on the basis of eukaryotic microorganisms—yeast—is the opportunity for correct modification of mammalian proteins. The cell surface display is an important tool for the analysis and understanding of protein function and protein–protein interactions and for the screening of novel clones from peptide and protein libraries. This technology makes it possible to obtain cells with novel abilities, such as catalytic functions and affinity binding to valuable ligands, including rare and heavy metals. It provides the chance to use yeast in biotechnology and in bioremediation and biomonitoring of the environment. The review considers the methods of obtaining a cell surface display on the basis of the yeasts Saccharomyces cerevisiae, Pichia pastoris, and Yarrowia lipolytica, the properties of anchor proteins, and the main fields of yeast display technology.

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KW - biomonitoring

KW - bioremediation

KW - immobilized enzymes stability

KW - metal recovery

KW - yeast surface display

KW - YARROWIA-LIPOLYTICA

KW - SURFACE-ENGINEERED YEAST

KW - ESCHERICHIA-COLI

KW - ETHANOL-PRODUCTION

KW - RECOMBINANT SACCHAROMYCES-CEREVISIAE

KW - WALL-ANCHORED PROTEIN

KW - PICHIA-PASTORIS

KW - RHIZOPUS-ORYZAE LIPASE

KW - BETA-GLUCOSIDASE

KW - ALPHA-AMYLASE

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DO - 10.1134/S0003683818040105

M3 - Review article

VL - 54

SP - 337

EP - 351

JO - Applied Biochemistry and Microbiology

JF - Applied Biochemistry and Microbiology

SN - 0003-6838

IS - 4

ER -