Photochemical activity changes accompanying the embryogenesis of pea (Pisum sativum) with yellow and green cotyledons

Переведенное название: Изменение фотохимической активности в процессе эмбриогенеза гороха (Pisum sativum L.) с желтыми и зелеными семядолями

G. Smolikova, V. Kreslavski, O. Shiroglazova, T. Bilova, E. Sharova, A. Frolov, S. Medvedev

Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

2 Цитирования (Scopus)

Выдержка

The pea seeds are photosynthetically active until the end of the maturation phase, when the embryonic chlorophylls degrade. However, in some cultivars, the underlying mechanisms are compromised, and the mature seeds preserve green colour. The residual chlorophylls can enhance oxidative degradation of reserve biomolecules, and affect thereby the quality, shelf life and nutritive value of seeds. Despite this, the formation, degradation, and physical properties of the seed chlorophylls are still not completely characterised. So here we address the dynamics of seed photochemical activity in the yellow- and green-seeded pea cultivars by the pulse amplitude modulation (PAM) fluorometric analysis. The experiments revealed the maximal photochemical activity at the early- and mid-cotyledon stages. Thereby, the active centres of PSII were saturated at the light intensity of 15-20μmol photons m-2 s-1. Despite of their shielding from the light by the pod wall and seed coat, photochemical reactions can be registered in the seeds with green embryo. Importantly, even at the low light intensities, the photochemical activity in the coats and cotyledons could be detected. The fast transients of the chlorophyll a fluorescence revealed a higher photochemical activity in the coat of yellow-seeded cultivars in comparison to those with the green-seeded ones. However, it declined rapidly in all seeds at the late cotyledon stage, and was accompanied with the decrease of the seed water content. Thus, the termination of photosynthetic activity in seeds is triggered by their dehydration.
Язык оригиналаанглийский
Страницы (с-по)228-235
Число страниц8
ЖурналFunctional Plant Biology
Том45
Номер выпуска1-2
DOI
СостояниеОпубликовано - 1 янв 2018

Ключевые слова

  • фотосинтез
  • эмбриогенез
  • горох
  • хлорофилл

Предметные области Scopus

  • Земледелие и биологические науки (все)
  • Биохимия, генетика и молекулярная биология (все)
  • Агрономия и растениеводство
  • Прикладная ботаника

Цитировать

@article{5b6ecaa6eb7b426f877c5d21ff565f32,
title = "Photochemical activity changes accompanying the embryogenesis of pea (Pisum sativum) with yellow and green cotyledons",
abstract = "The pea seeds are photosynthetically active until the end of the maturation phase, when the embryonic chlorophylls degrade. However, in some cultivars, the underlying mechanisms are compromised, and the mature seeds preserve green colour. The residual chlorophylls can enhance oxidative degradation of reserve biomolecules, and affect thereby the quality, shelf life and nutritive value of seeds. Despite this, the formation, degradation, and physical properties of the seed chlorophylls are still not completely characterised. So here we address the dynamics of seed photochemical activity in the yellow- and green-seeded pea cultivars by the pulse amplitude modulation (PAM) fluorometric analysis. The experiments revealed the maximal photochemical activity at the early- and mid-cotyledon stages. Thereby, the active centres of PSII were saturated at the light intensity of 15-20μmol photons m-2 s-1. Despite of their shielding from the light by the pod wall and seed coat, photochemical reactions can be registered in the seeds with green embryo. Importantly, even at the low light intensities, the photochemical activity in the coats and cotyledons could be detected. The fast transients of the chlorophyll a fluorescence revealed a higher photochemical activity in the coat of yellow-seeded cultivars in comparison to those with the green-seeded ones. However, it declined rapidly in all seeds at the late cotyledon stage, and was accompanied with the decrease of the seed water content. Thus, the termination of photosynthetic activity in seeds is triggered by their dehydration.",
keywords = "фотосинтез, эмбриогенез, горох, хлорофилл, chlorophyll, chlorophyll a fluorescence, embryogenesis, photosynthesis, seed development, STORAGE, LIGHT, CHLOROPHYLL B, SEEDS, CHLOROEMBRYOS, DEGRADATION, SENESCENCE, GRADIENTS, EMBRYOS, PHOTOSYNTHESIS",
author = "G. Smolikova and V. Kreslavski and O. Shiroglazova and T. Bilova and E. Sharova and A. Frolov and S. Medvedev",
note = "Smolikova G., Kreslavski V., Shiroglazova O., Sharova E., Bilova T., Frolov A., Medvedev S. Рhotochemical activity changes accompanying the embryogenesis of pea (Pisum sativum L.) with yellow and green cotyledons // Functional Plant Biology. 2018. V. 45. No 1-2. P. 228-235.",
year = "2018",
month = "1",
day = "1",
doi = "10.1071/FP16379",
language = "English",
volume = "45",
pages = "228--235",
journal = "Functional Plant Biology",
issn = "1445-4408",
publisher = "CSIRO",
number = "1-2",

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Photochemical activity changes accompanying the embryogenesis of pea (Pisum sativum) with yellow and green cotyledons. / Smolikova, G.; Kreslavski, V.; Shiroglazova, O.; Bilova, T.; Sharova, E.; Frolov, A.; Medvedev, S.

В: Functional Plant Biology, Том 45, № 1-2, 01.01.2018, стр. 228-235.

Результат исследований: Научные публикации в периодических изданияхстатьянаучнаярецензирование

TY - JOUR

T1 - Photochemical activity changes accompanying the embryogenesis of pea (Pisum sativum) with yellow and green cotyledons

AU - Smolikova, G.

AU - Kreslavski, V.

AU - Shiroglazova, O.

AU - Bilova, T.

AU - Sharova, E.

AU - Frolov, A.

AU - Medvedev, S.

N1 - Smolikova G., Kreslavski V., Shiroglazova O., Sharova E., Bilova T., Frolov A., Medvedev S. Рhotochemical activity changes accompanying the embryogenesis of pea (Pisum sativum L.) with yellow and green cotyledons // Functional Plant Biology. 2018. V. 45. No 1-2. P. 228-235.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The pea seeds are photosynthetically active until the end of the maturation phase, when the embryonic chlorophylls degrade. However, in some cultivars, the underlying mechanisms are compromised, and the mature seeds preserve green colour. The residual chlorophylls can enhance oxidative degradation of reserve biomolecules, and affect thereby the quality, shelf life and nutritive value of seeds. Despite this, the formation, degradation, and physical properties of the seed chlorophylls are still not completely characterised. So here we address the dynamics of seed photochemical activity in the yellow- and green-seeded pea cultivars by the pulse amplitude modulation (PAM) fluorometric analysis. The experiments revealed the maximal photochemical activity at the early- and mid-cotyledon stages. Thereby, the active centres of PSII were saturated at the light intensity of 15-20μmol photons m-2 s-1. Despite of their shielding from the light by the pod wall and seed coat, photochemical reactions can be registered in the seeds with green embryo. Importantly, even at the low light intensities, the photochemical activity in the coats and cotyledons could be detected. The fast transients of the chlorophyll a fluorescence revealed a higher photochemical activity in the coat of yellow-seeded cultivars in comparison to those with the green-seeded ones. However, it declined rapidly in all seeds at the late cotyledon stage, and was accompanied with the decrease of the seed water content. Thus, the termination of photosynthetic activity in seeds is triggered by their dehydration.

AB - The pea seeds are photosynthetically active until the end of the maturation phase, when the embryonic chlorophylls degrade. However, in some cultivars, the underlying mechanisms are compromised, and the mature seeds preserve green colour. The residual chlorophylls can enhance oxidative degradation of reserve biomolecules, and affect thereby the quality, shelf life and nutritive value of seeds. Despite this, the formation, degradation, and physical properties of the seed chlorophylls are still not completely characterised. So here we address the dynamics of seed photochemical activity in the yellow- and green-seeded pea cultivars by the pulse amplitude modulation (PAM) fluorometric analysis. The experiments revealed the maximal photochemical activity at the early- and mid-cotyledon stages. Thereby, the active centres of PSII were saturated at the light intensity of 15-20μmol photons m-2 s-1. Despite of their shielding from the light by the pod wall and seed coat, photochemical reactions can be registered in the seeds with green embryo. Importantly, even at the low light intensities, the photochemical activity in the coats and cotyledons could be detected. The fast transients of the chlorophyll a fluorescence revealed a higher photochemical activity in the coat of yellow-seeded cultivars in comparison to those with the green-seeded ones. However, it declined rapidly in all seeds at the late cotyledon stage, and was accompanied with the decrease of the seed water content. Thus, the termination of photosynthetic activity in seeds is triggered by their dehydration.

KW - фотосинтез

KW - эмбриогенез

KW - горох

KW - хлорофилл

KW - chlorophyll

KW - chlorophyll a fluorescence

KW - embryogenesis

KW - photosynthesis

KW - seed development

KW - STORAGE

KW - LIGHT

KW - CHLOROPHYLL B

KW - SEEDS

KW - CHLOROEMBRYOS

KW - DEGRADATION

KW - SENESCENCE

KW - GRADIENTS

KW - EMBRYOS

KW - PHOTOSYNTHESIS

UR - http://www.scopus.com/inward/record.url?scp=85040187432&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/photochemical-activity-changes-accompanying-embryogenesis-pea-pisum-sativum-yellow-green-cotyledons

U2 - 10.1071/FP16379

DO - 10.1071/FP16379

M3 - Article

VL - 45

SP - 228

EP - 235

JO - Functional Plant Biology

JF - Functional Plant Biology

SN - 1445-4408

IS - 1-2

ER -