Are there any species-specific sperm proteins in periwinkles?

Переведенное название: Есть ли какие-либо видоспецифичные спермальные белки у литоральных моллюсков?

Результат исследований: Материалы конференцийтезисы

11 Загрузки (Pure)

Аннотация

Population ranges of different Littorina species overlap, allowing not species-specific copulations and incomplete reproductive isolation between some of them. This poses question how these coexisting related species maintain their identity. Gamete incompatibility is one of the possible mechanisms of
reproductive isolation in such system. In this case, a search for acrosomal
proteins that are responsible for the specificity of fertilization can be a promising direction. In some taxa (e.g. abalones and sea urchins) species-specific acrosomal proteins were studied. Mutations in these proteins are supposed to cause speciation in these groups. Thus, the search for acrosomal proteins advances our understanding of the reproductive isolation mechanisms and speciation in genus Littorina. For present study we obtained reproductive tissue extracts, total lysates of different body parts and phenolic fixations for 5 periwinkle species (L.obtusata, L.littorea, L.saxatilis, L.arcana, L.compressa), and acrosomal extract only for L.obtusata. The protein samples were analyzed using proteomics methods (1D and 2D-electrophoresis and «bottom up» analysis). We were able to identify a sperm-specific protein of L.obtusata with an approximate
weight of 17 kDa. Edman sequencing allowed identifying its first 10 amino acids. Based on this sequence we made degenerate primers and used them for 3'-RACE. Finally, we identified a reproductive-specific transcript of the target protein that shows no homology with any known proteins. This protein is a species-specific marker of L.obtusata. Its acrosomal localization and
involvement into reproductive isolation process need further examination
Переведенное названиеЕсть ли какие-либо видоспецифичные спермальные белки у литоральных моллюсков?
Язык оригиналаанглийский
Страницы28
Число страниц48
СостояниеОпубликовано - 28 июл 2014
СобытиеInternationol Symposium on Littorinid Biology and Evolution - Hong Kong, Китай
Продолжительность: 28 июл 20141 авг 2014
Номер конференции: 11
https://pure.spbu.ru/ws/portalfiles/portal/19067484

конференция

конференцияInternationol Symposium on Littorinid Biology and Evolution
СтранаКитай
ГородHong Kong
Период28/07/141/08/14
Адрес в сети Интернет

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