Biological activity of analogs of the peptide hormone luliberin in the regulation of the immune response of T cells

Переведенное название: Биологическая активность аналогов пептидного гормона - люлиберина в регуляции иммунного ответа Т-лимфоцитов

T. B. Kazakova, S. V. Burov, T. V. Grishina, A. A. Myul'berg, E. A. Korneva, N.S. Novikova, T.V. Semko, O.I. Golovko

Результат исследований: Научные публикации в периодических изданияхстатья

Аннотация

The ability of the antitumor analogs of luliberin (LH-RH), a hypothalamic peptide hormone, to stimulate the immune function of T cells is examined in experiments with the gene coding for interleukin-2 (IL-2). Recombinant MIL2C or 4xPu DNA containing the marker gene of chloramphenicol acetyltransferase (CAT) under the control of a 2.2 kb promoter of murine IL-2 gene or four copies of purine-rich element (from -292 to -246 base pairs), respectively, is injected in Xenopus laevis oocytes. The promoter activity is blocked by inoculation of the protein fraction of nuclear extracts from resting mouse splenic T cells. The IL-2 gene promoter is dereppressed after injection of the short LH-RH analog L1 (7 amino acid residues) into the oocyte nucleus or cytoplasm. The addition of L1 or L2 (an LH-RH analog consisting of 10 amino acid residues) to the incubation medium activates mouse splenic T cells and stimulates the synthesis of IL-2 mRNA 2-to 3-fold more intensely than ConA+rIL-2, judging from dot-blot and in situ hybridization data. Cytological analysis of cell culture shows that the presence of L1 and L2 peptides in the culture medium promotes differentiation of T cells. It is hypothesized that the antitumor activity of these peptides is associated with the stimulation of IL-2 synthesis.

Язык оригиналаанглийский
Страницы (с-по)943-946
Число страниц4
ЖурналBulletin of Experimental Biology and Medicine
Том122
Номер выпуска9
СостояниеОпубликовано - 1 сен 1996

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Предметные области Scopus

  • Биохимия, генетика и молекулярная биология (все)

Ключевые слова

  • люлиберин, ген интерлейкина-2, синтез мРНК

Цитировать

Kazakova, T. B., Burov, S. V., Grishina, T. V., Myul'berg, A. A., Korneva, E. A., Novikova, N. S., ... Golovko, O. I. (1996). Biological activity of analogs of the peptide hormone luliberin in the regulation of the immune response of T cells. Bulletin of Experimental Biology and Medicine, 122(9), 943-946.