Sup35 prion protein, a translation termination factor (eRF3) of yeast Saccharomyces cerevisiae has been shown to form both transmissible amyloid fibrils and reversible biomolecular condensates. The Sup35N region, containing IDRs, is sufficient for both LLPS and amyloid aggregation, however the roles of specific regions of N-domain in LLPS remained uncertain. The goal of this study is to determine sequence elements responsible for phase separation.