Based on a study of pyrite from the Uzelga Cu-Zn volcanogenic massive sulfide (VMS) deposit (South Urals) the age of ore mineralization was first determined with the direct age-dating method, based on the fraction of radiogenic helium, incorporated into the pyrite crystal lattice from submicron inclusions of U and Th minerals. Taking into account the measurement errors, the obtained age of 377 ± 8 Ma (MSWD = 1.2) is quite consistent with the independent age dates available for the ore mineralization (Late Eifelian-Early Givetian, 385-390 Ma).