Urethroplasty with a bilayered poly-D,L-lactide-co-ε-caprolactone scaffold seeded with allogenic mesenchymal stem cells

Natalia M. Yudintceva, Yulia A. Nashchekina, Nataliya A. Mikhailova, Tatiana I. Vinogradova, Petr K. Yablonsky, Anna A. Gorelova, Alexandr N. Muraviov, Andrey V. Gorelov, Igor A. Samusenko, Boris P. Nikolaev, Ludmila Y. Yakovleva, Maxim A. Shevtsov

Research output

Abstract

Reconstructive surgery for urethral defects employing tissue-engineered scaffolds represents an alternative treatment for urethroplasty. The aim of this study was to compare the therapeutic efficacy of the bilayer poly-D,L-lactide/poly-ε-caprolactone (PL-PC) scaffold seeded with allogenic mesenchymal stem cells (MSCs) for urethra reconstruction in a rabbit model with conventional urethroplasty employing an autologous buccal mucosa graft (BG). The inner layer of the scaffold based on poly-D,L-lactic acid (PL) was seeded with MSCs, while the outer layer, prepared from poly-ε-caprolactone, protected the surrounding tissues from urine. To track the MSCs in vivo, the latter were labeled with superparamagnetic iron oxide nanoparticles. In rabbits, a dorsal penile defect was reconstructed employing a BG or a PL-PC graft seeded with nanoparticle-labeled MSCs. In the 12-week follow-up period, no complications were detected. Subsequent histological analysis demonstrated biointegration of the PL-PC graft with surrounding urethral tissues. Less fibrosis and inflammatory cell infiltration were observed in the experimental group as compared with the BG group. Nanoparticle-labeled MSCs were detected in the urothelium and muscular layer, co-localizing with the urothelium cytokeratin marker AE1/AE3, indicating the possibility of MSC differentiation into neo-urothelium. Our results suggest that a bilayer MSCs-seeded scaffold could be efficiently employed for urethroplasty.

Original languageEnglish
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
DOIs
Publication statusPublished - 2019

Fingerprint

Stem cells
Scaffolds
Grafts
Nanoparticles
Tissue
Tissue Scaffolds
Defects
Lactic acid
Keratins
caprolactone
poly(lactide)
Iron oxides
Infiltration
Surgery
Lactic Acid
polycaprolactone
Mucous Membrane

Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Yudintceva, Natalia M. ; Nashchekina, Yulia A. ; Mikhailova, Nataliya A. ; Vinogradova, Tatiana I. ; Yablonsky, Petr K. ; Gorelova, Anna A. ; Muraviov, Alexandr N. ; Gorelov, Andrey V. ; Samusenko, Igor A. ; Nikolaev, Boris P. ; Yakovleva, Ludmila Y. ; Shevtsov, Maxim A. / Urethroplasty with a bilayered poly-D,L-lactide-co-ε-caprolactone scaffold seeded with allogenic mesenchymal stem cells. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2019.
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title = "Urethroplasty with a bilayered poly-D,L-lactide-co-ε-caprolactone scaffold seeded with allogenic mesenchymal stem cells",
abstract = "Reconstructive surgery for urethral defects employing tissue-engineered scaffolds represents an alternative treatment for urethroplasty. The aim of this study was to compare the therapeutic efficacy of the bilayer poly-D,L-lactide/poly-ε-caprolactone (PL-PC) scaffold seeded with allogenic mesenchymal stem cells (MSCs) for urethra reconstruction in a rabbit model with conventional urethroplasty employing an autologous buccal mucosa graft (BG). The inner layer of the scaffold based on poly-D,L-lactic acid (PL) was seeded with MSCs, while the outer layer, prepared from poly-ε-caprolactone, protected the surrounding tissues from urine. To track the MSCs in vivo, the latter were labeled with superparamagnetic iron oxide nanoparticles. In rabbits, a dorsal penile defect was reconstructed employing a BG or a PL-PC graft seeded with nanoparticle-labeled MSCs. In the 12-week follow-up period, no complications were detected. Subsequent histological analysis demonstrated biointegration of the PL-PC graft with surrounding urethral tissues. Less fibrosis and inflammatory cell infiltration were observed in the experimental group as compared with the BG group. Nanoparticle-labeled MSCs were detected in the urothelium and muscular layer, co-localizing with the urothelium cytokeratin marker AE1/AE3, indicating the possibility of MSC differentiation into neo-urothelium. Our results suggest that a bilayer MSCs-seeded scaffold could be efficiently employed for urethroplasty.",
keywords = "bilayer scaffold, buccal graft, mesenchymal stem cells, superparamagnetic iron oxide nanoparticles, urethral substitution",
author = "Yudintceva, {Natalia M.} and Nashchekina, {Yulia A.} and Mikhailova, {Nataliya A.} and Vinogradova, {Tatiana I.} and Yablonsky, {Petr K.} and Gorelova, {Anna A.} and Muraviov, {Alexandr N.} and Gorelov, {Andrey V.} and Samusenko, {Igor A.} and Nikolaev, {Boris P.} and Yakovleva, {Ludmila Y.} and Shevtsov, {Maxim A.}",
year = "2019",
doi = "10.1002/jbm.b.34453",
language = "English",
journal = "Journal of Biomedical Materials Research - Part B Applied Biomaterials",
issn = "1552-4973",
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Urethroplasty with a bilayered poly-D,L-lactide-co-ε-caprolactone scaffold seeded with allogenic mesenchymal stem cells. / Yudintceva, Natalia M.; Nashchekina, Yulia A.; Mikhailova, Nataliya A.; Vinogradova, Tatiana I.; Yablonsky, Petr K.; Gorelova, Anna A.; Muraviov, Alexandr N.; Gorelov, Andrey V.; Samusenko, Igor A.; Nikolaev, Boris P.; Yakovleva, Ludmila Y.; Shevtsov, Maxim A.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, 2019.

Research output

TY - JOUR

T1 - Urethroplasty with a bilayered poly-D,L-lactide-co-ε-caprolactone scaffold seeded with allogenic mesenchymal stem cells

AU - Yudintceva, Natalia M.

AU - Nashchekina, Yulia A.

AU - Mikhailova, Nataliya A.

AU - Vinogradova, Tatiana I.

AU - Yablonsky, Petr K.

AU - Gorelova, Anna A.

AU - Muraviov, Alexandr N.

AU - Gorelov, Andrey V.

AU - Samusenko, Igor A.

AU - Nikolaev, Boris P.

AU - Yakovleva, Ludmila Y.

AU - Shevtsov, Maxim A.

PY - 2019

Y1 - 2019

N2 - Reconstructive surgery for urethral defects employing tissue-engineered scaffolds represents an alternative treatment for urethroplasty. The aim of this study was to compare the therapeutic efficacy of the bilayer poly-D,L-lactide/poly-ε-caprolactone (PL-PC) scaffold seeded with allogenic mesenchymal stem cells (MSCs) for urethra reconstruction in a rabbit model with conventional urethroplasty employing an autologous buccal mucosa graft (BG). The inner layer of the scaffold based on poly-D,L-lactic acid (PL) was seeded with MSCs, while the outer layer, prepared from poly-ε-caprolactone, protected the surrounding tissues from urine. To track the MSCs in vivo, the latter were labeled with superparamagnetic iron oxide nanoparticles. In rabbits, a dorsal penile defect was reconstructed employing a BG or a PL-PC graft seeded with nanoparticle-labeled MSCs. In the 12-week follow-up period, no complications were detected. Subsequent histological analysis demonstrated biointegration of the PL-PC graft with surrounding urethral tissues. Less fibrosis and inflammatory cell infiltration were observed in the experimental group as compared with the BG group. Nanoparticle-labeled MSCs were detected in the urothelium and muscular layer, co-localizing with the urothelium cytokeratin marker AE1/AE3, indicating the possibility of MSC differentiation into neo-urothelium. Our results suggest that a bilayer MSCs-seeded scaffold could be efficiently employed for urethroplasty.

AB - Reconstructive surgery for urethral defects employing tissue-engineered scaffolds represents an alternative treatment for urethroplasty. The aim of this study was to compare the therapeutic efficacy of the bilayer poly-D,L-lactide/poly-ε-caprolactone (PL-PC) scaffold seeded with allogenic mesenchymal stem cells (MSCs) for urethra reconstruction in a rabbit model with conventional urethroplasty employing an autologous buccal mucosa graft (BG). The inner layer of the scaffold based on poly-D,L-lactic acid (PL) was seeded with MSCs, while the outer layer, prepared from poly-ε-caprolactone, protected the surrounding tissues from urine. To track the MSCs in vivo, the latter were labeled with superparamagnetic iron oxide nanoparticles. In rabbits, a dorsal penile defect was reconstructed employing a BG or a PL-PC graft seeded with nanoparticle-labeled MSCs. In the 12-week follow-up period, no complications were detected. Subsequent histological analysis demonstrated biointegration of the PL-PC graft with surrounding urethral tissues. Less fibrosis and inflammatory cell infiltration were observed in the experimental group as compared with the BG group. Nanoparticle-labeled MSCs were detected in the urothelium and muscular layer, co-localizing with the urothelium cytokeratin marker AE1/AE3, indicating the possibility of MSC differentiation into neo-urothelium. Our results suggest that a bilayer MSCs-seeded scaffold could be efficiently employed for urethroplasty.

KW - bilayer scaffold

KW - buccal graft

KW - mesenchymal stem cells

KW - superparamagnetic iron oxide nanoparticles

KW - urethral substitution

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U2 - 10.1002/jbm.b.34453

DO - 10.1002/jbm.b.34453

M3 - Article

AN - SCOPUS:85070084643

JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials

JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials

SN - 1552-4973

ER -