The Thermus thermophilus 5S rRNA-protein complex: Identification of specific binding sites for proteins L5 and L18 in the 5S rRNA

G. M. Gongadze, A. A. Perederina, V. A. Meshcheryakov, R. V. Fedorov, S. E. Moskalenko, A. V. Rak, A. A. Serganov, D. V. Shcherbakov, S. V. Nikonov, M. B. Garber

Research output

4 Citations (Scopus)

Abstract

Three 5S rRNA-binding ribosomal proteins (L5, L18, TL5) of extremely thermophilic bacterium Thermus thermophilus have earlier been isolated. Structural analysis of their complexes with rRNA requires identification of their binding sites in the 5S rRNA. Previously, a TL5-binding site has been identified, a TL5-RNA complex crystallized, and its structure determined to 2.3 Å. The sites for L5 and L18 were characterized, and two corresponding 5S rRNA fragments constructed. Of these, a 34-nt fragment specifically interacted with L5, and a 55-nt fragment interacted with L5, L18, and with both proteins. The 34-nt fragment-L5 complex was crystallized; the crystals are suitable for high-resolution X-ray analysis.

Original languageEnglish
Pages (from-to)521-526
Number of pages6
JournalMolecular Biology
Volume35
Issue number4
DOIs
Publication statusPublished - 1 Jul 2001

Fingerprint

Thermus thermophilus
Carrier Proteins
Binding Sites
Proteins
X-Rays
RNA
Bacteria
ribosomal protein L5
ribosomal protein L18

Scopus subject areas

  • Biophysics
  • Structural Biology

Cite this

Gongadze, G. M. ; Perederina, A. A. ; Meshcheryakov, V. A. ; Fedorov, R. V. ; Moskalenko, S. E. ; Rak, A. V. ; Serganov, A. A. ; Shcherbakov, D. V. ; Nikonov, S. V. ; Garber, M. B. / The Thermus thermophilus 5S rRNA-protein complex : Identification of specific binding sites for proteins L5 and L18 in the 5S rRNA. In: Molecular Biology. 2001 ; Vol. 35, No. 4. pp. 521-526.
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abstract = "Three 5S rRNA-binding ribosomal proteins (L5, L18, TL5) of extremely thermophilic bacterium Thermus thermophilus have earlier been isolated. Structural analysis of their complexes with rRNA requires identification of their binding sites in the 5S rRNA. Previously, a TL5-binding site has been identified, a TL5-RNA complex crystallized, and its structure determined to 2.3 {\AA}. The sites for L5 and L18 were characterized, and two corresponding 5S rRNA fragments constructed. Of these, a 34-nt fragment specifically interacted with L5, and a 55-nt fragment interacted with L5, L18, and with both proteins. The 34-nt fragment-L5 complex was crystallized; the crystals are suitable for high-resolution X-ray analysis.",
keywords = "5S rRNA, Bacterial ribosome, Ribosomal proteins, RNA-protein interactions, Thermus thermophilus",
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The Thermus thermophilus 5S rRNA-protein complex : Identification of specific binding sites for proteins L5 and L18 in the 5S rRNA. / Gongadze, G. M.; Perederina, A. A.; Meshcheryakov, V. A.; Fedorov, R. V.; Moskalenko, S. E.; Rak, A. V.; Serganov, A. A.; Shcherbakov, D. V.; Nikonov, S. V.; Garber, M. B.

In: Molecular Biology, Vol. 35, No. 4, 01.07.2001, p. 521-526.

Research output

TY - JOUR

T1 - The Thermus thermophilus 5S rRNA-protein complex

T2 - Identification of specific binding sites for proteins L5 and L18 in the 5S rRNA

AU - Gongadze, G. M.

AU - Perederina, A. A.

AU - Meshcheryakov, V. A.

AU - Fedorov, R. V.

AU - Moskalenko, S. E.

AU - Rak, A. V.

AU - Serganov, A. A.

AU - Shcherbakov, D. V.

AU - Nikonov, S. V.

AU - Garber, M. B.

PY - 2001/7/1

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AB - Three 5S rRNA-binding ribosomal proteins (L5, L18, TL5) of extremely thermophilic bacterium Thermus thermophilus have earlier been isolated. Structural analysis of their complexes with rRNA requires identification of their binding sites in the 5S rRNA. Previously, a TL5-binding site has been identified, a TL5-RNA complex crystallized, and its structure determined to 2.3 Å. The sites for L5 and L18 were characterized, and two corresponding 5S rRNA fragments constructed. Of these, a 34-nt fragment specifically interacted with L5, and a 55-nt fragment interacted with L5, L18, and with both proteins. The 34-nt fragment-L5 complex was crystallized; the crystals are suitable for high-resolution X-ray analysis.

KW - 5S rRNA

KW - Bacterial ribosome

KW - Ribosomal proteins

KW - RNA-protein interactions

KW - Thermus thermophilus

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