The study of microphylls formation molecular mechanisms within the Huperzia selago (L.) Bernh. ex Schrank et Mart. (Lycopodiophyta) shoot apical meristem

Марина Андреевна Романова, Anastasia Evkaikina, Alexandra Ivanova, Lidia Berke, Catharina Rydin, Katharina Pawlovski, Olga Voitsekhovskaya

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The Lycopodiophyta represent the oldest lineage among the extant vascular plants. They represent a sister group to all other vascular plants, from which they differ in that their leaves are microphylls, not megaphylls. Different subgroups of the Lycopodiophyta are distinguished by their type of shoot apical meristem (SAM): the representatives of Selaginellales possess monoplex SAMs and those from Lycopodiales simplex SAMs. The simplex lycopod SAM resembles the SAM of seed plants. In vascular plants, both microphylls and megaphylls emerge within the peripheral zone of the SAM. Molecular-genetic mechanisms of leaf formation have been thoroughly studied among the Magnoliophyta, where leaf initiation is determined by the antagonism between the KNOX transcription factors (TF), which determine cell fate indeterminancy, and the transcription factors ARP and YABBY which are responsible for determinacy and thus required for the identification of leaf primordium founder cells. Since in the only lycopod genome available, of Selaginella
IV (XII) International Botanical Conference of Young Scientists
moellendorffii, ARP homologs are present and YABBY homologs are absent, it has been postulated that YABBY genes are unique to seed plants. We generated a shoot tip transcriptome of Huperzia selago (Lycopodiales) to examine the genes involved in microphyll leaf formation in the simpex SAM. We discovered the presence of HsYABBY expression and the absence of any ARP gene expression activity in H. selago shoot tips. Phylogenetic analysis showed that HsYABBY gene is a sister to YABBY proteins from euphyllophytes. Thus, YABBY genes have been appeared in the last common ancestor of vascular plants. Localization of HsYABBY transcripts in the H. selago SAM and the comparison with the expression pattern of ARP in the monoplex SAM of Selaginella kraussiana based on literature data showed that the development of microphylls does not require the combined activities of ARP and YABBY.
Original languageRussian
Number of pages2
StatePublished - 22 Apr 2018

Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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