The plastid ndh genes code for an NADH-specific dehydrogenase - isolation of a complex I analogue from pea thylakoid membranes

L.A. Sazanov, P.A. Burrows, P.J. Nixon

Research output

150 Citations (Scopus)

Abstract

The plastid genomes of several plants contain ndh genes-homologues of genes encoding subunits of the proton-pumping NADH:ubiquinone oxidoreductase, or complex I, involved in respiration in mitochondria and eubacteria. From sequence similarities with these genes, the ndh gene products have been suggested to form a large protein complex (Ndh complex); however, the structure and function of this complex remains to be established. Herein we report the isolation of the Ndh complex from the chloroplasts of the higher plant Pisum sativum. The purification procedure involved selective solubilization of the thylakoid membrane with dodecyl maltoside, followed by two anion-exchange chromatography steps and one size-exclusion chromatography step. The isolated Ndh complex has an apparent total molecular mass of approximately 550 kDa and according to SDS/PAGE consists of at least 16 subunits including NdhA, NdhI, NdhJ, NdhK, and NdhH, which were identified by N-terminal sequencing and immunoblotting. The Ndh complex showed
Original languageEnglish
Pages (from-to)1319-1324
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number3
Publication statusPublished - 1998
Externally publishedYes

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