The Genome of Paramecium caudatum: Analysis by Means of Pulsed Field Gel Electrophoresis

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Abstract

Electrokaryotypes of eleven clones of the ciliated protozoan Paramecium caudatum were obtained by means of pulsed field gel electrophoresis (PFGE) and analyzed using the densitometry and Southern blot hybridization techniques. The main characteristics of the spectra of macronuclear DNA fragments from different clones were identical. Southern hybridization with the sequences complementary to the telomerase RNA component and the calmodulin gene showed that these genes were located in macronuclear DNA fragments of similar size in all clones studied. The interclone polymorphism was demonstrated only for the number and position of the bands corresponding to hyperamplified molecules. Quantitative analysis of the macronuclear DNA spectra from different P. caudatum clones was conducted. The total number of DNA molecules per macronuclear subgenome was estimated at 230 ± 40. The kinetic complexity of a macronuclear genome is discussed.

Original languageEnglish
Pages (from-to)1413-1420
Number of pages8
JournalRussian Journal of Genetics
Volume35
Issue number12
Publication statusPublished - 1 Dec 1999

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Paramecium caudatum
Pulsed Field Gel Electrophoresis
Clone Cells
Genome
DNA
Densitometry
Calmodulin
Southern Blotting
Genes

Scopus subject areas

  • Genetics

Cite this

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title = "The Genome of Paramecium caudatum: Analysis by Means of Pulsed Field Gel Electrophoresis",
abstract = "Electrokaryotypes of eleven clones of the ciliated protozoan Paramecium caudatum were obtained by means of pulsed field gel electrophoresis (PFGE) and analyzed using the densitometry and Southern blot hybridization techniques. The main characteristics of the spectra of macronuclear DNA fragments from different clones were identical. Southern hybridization with the sequences complementary to the telomerase RNA component and the calmodulin gene showed that these genes were located in macronuclear DNA fragments of similar size in all clones studied. The interclone polymorphism was demonstrated only for the number and position of the bands corresponding to hyperamplified molecules. Quantitative analysis of the macronuclear DNA spectra from different P. caudatum clones was conducted. The total number of DNA molecules per macronuclear subgenome was estimated at 230 ± 40. The kinetic complexity of a macronuclear genome is discussed.",
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T1 - The Genome of Paramecium caudatum: Analysis by Means of Pulsed Field Gel Electrophoresis

AU - Potekhin, A. A.

AU - Rautian, M. S.

AU - Brigge, T.

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Y1 - 1999/12/1

N2 - Electrokaryotypes of eleven clones of the ciliated protozoan Paramecium caudatum were obtained by means of pulsed field gel electrophoresis (PFGE) and analyzed using the densitometry and Southern blot hybridization techniques. The main characteristics of the spectra of macronuclear DNA fragments from different clones were identical. Southern hybridization with the sequences complementary to the telomerase RNA component and the calmodulin gene showed that these genes were located in macronuclear DNA fragments of similar size in all clones studied. The interclone polymorphism was demonstrated only for the number and position of the bands corresponding to hyperamplified molecules. Quantitative analysis of the macronuclear DNA spectra from different P. caudatum clones was conducted. The total number of DNA molecules per macronuclear subgenome was estimated at 230 ± 40. The kinetic complexity of a macronuclear genome is discussed.

AB - Electrokaryotypes of eleven clones of the ciliated protozoan Paramecium caudatum were obtained by means of pulsed field gel electrophoresis (PFGE) and analyzed using the densitometry and Southern blot hybridization techniques. The main characteristics of the spectra of macronuclear DNA fragments from different clones were identical. Southern hybridization with the sequences complementary to the telomerase RNA component and the calmodulin gene showed that these genes were located in macronuclear DNA fragments of similar size in all clones studied. The interclone polymorphism was demonstrated only for the number and position of the bands corresponding to hyperamplified molecules. Quantitative analysis of the macronuclear DNA spectra from different P. caudatum clones was conducted. The total number of DNA molecules per macronuclear subgenome was estimated at 230 ± 40. The kinetic complexity of a macronuclear genome is discussed.

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