The Effects of Severe Hypobaric Hypoxia and Inhibition of Hypoxia-Inducible Factor-1 (HIF-1) on Biomarkers of Cardiac and Skeletal Muscle Injury in Rats// ISSN 0006-3509, Biophysics, 2019, Vol. 64, No. 5, pp. 808–811. DOI 10.1134/S000635091905004X ( Scopus, ВАК, article is available as 'Online First': http://link.springer.com/article/10.1134/S000635091905004X )

Research output

Abstract

The goal of the present study was to investigate the molecular mechanisms that underlie heart and
skeletal muscle damage in male Wistar rats weighing 200–250 g in response to a 3-h exposure to 180 mm Hg
(5% O2) in the model of severe hypobaric hypoxia. It has been demonstrated that the level of the cardiac biomarker
troponin I in the blood plasma of rats exposed to severe hypobaric hypoxia for 3 h increased significantly
compared to the control group, indicating myocardial injury. At the same time, the administration of
the HIF-1α transcription factor inhibitor did not affect the plasma level of troponin I. In contrast, the release
of the non-specific biomarker myoglobin into the bloodstream did not increase in response to hypoxia compared
to the control animals. In addition, 24 h after the exposure to severe hypobaric hypoxia the serum myoglobin
level was significantly lower in animals administered with the HIF-1α inhibitor topotecan than in rats
that did not receive topotecan. Therefore, it may be assumed that the inhibition of the HIF-1α transcription
factor 10 min before exposure to severe hypobaric hypoxia reduces skeletal muscle damage. The mechanisms
that affect the adaptation of heart and skeletal muscles to hypoxia are discussed.
Original languageEnglish
Article numberDOI 10.1134/S000635091905004X
Pages (from-to)808-811
Number of pages10
JournalBiophysics (Russian Federation)
Volume64
Issue number5
Publication statusPublished - 2019

Cite this

@article{c00c70aec6ea42d4b71be6b776a39b91,
title = "The Effects of Severe Hypobaric Hypoxia and Inhibition of Hypoxia-Inducible Factor-1 (HIF-1) on Biomarkers of Cardiac and Skeletal Muscle Injury in Rats// ISSN 0006-3509, Biophysics, 2019, Vol. 64, No. 5, pp. 808–811. DOI 10.1134/S000635091905004X ( Scopus, ВАК, article is available as 'Online First': http://link.springer.com/article/10.1134/S000635091905004X )",
abstract = "The goal of the present study was to investigate the molecular mechanisms that underlie heart andskeletal muscle damage in male Wistar rats weighing 200–250 g in response to a 3-h exposure to 180 mm Hg(5{\%} O2) in the model of severe hypobaric hypoxia. It has been demonstrated that the level of the cardiac biomarkertroponin I in the blood plasma of rats exposed to severe hypobaric hypoxia for 3 h increased significantlycompared to the control group, indicating myocardial injury. At the same time, the administration ofthe HIF-1α transcription factor inhibitor did not affect the plasma level of troponin I. In contrast, the releaseof the non-specific biomarker myoglobin into the bloodstream did not increase in response to hypoxia comparedto the control animals. In addition, 24 h after the exposure to severe hypobaric hypoxia the serum myoglobinlevel was significantly lower in animals administered with the HIF-1α inhibitor topotecan than in ratsthat did not receive topotecan. Therefore, it may be assumed that the inhibition of the HIF-1α transcriptionfactor 10 min before exposure to severe hypobaric hypoxia reduces skeletal muscle damage. The mechanismsthat affect the adaptation of heart and skeletal muscles to hypoxia are discussed.",
keywords = "hypoxia, myoglobin, troponin I, topotecan,hypoxia-inducible factor 1-alpha (HIF-1α), myocardium, myoglobin",
author = "Федоров, {Даниил Александрович} and Фролова, {Милена Юрьевна} and Красовская, {Ирина Евгеньевна} and Кулева, {Надежда Владимировна}",
note = "Fedorov, D.A., Frolova, M.Y., Krasovskaya, I.E. et al. The Effects of Severe Hypobaric Hypoxia and Inhibition of Hypoxia-Inducible Factor-1 (HIF-1) on Biomarkers of Cardiac and Skeletal Muscle Injury in Rats. BIOPHYSICS 64, 808–811 (2019) doi:10.1134/S000635091905004X",
year = "2019",
language = "English",
volume = "64",
pages = "808--811",
journal = "Biophysics (Russian Federation)",
issn = "0006-3509",
publisher = "Springer",
number = "5",

}

TY - JOUR

T1 - The Effects of Severe Hypobaric Hypoxia and Inhibition of Hypoxia-Inducible Factor-1 (HIF-1) on Biomarkers of Cardiac and Skeletal Muscle Injury in Rats// ISSN 0006-3509, Biophysics, 2019, Vol. 64, No. 5, pp. 808–811. DOI 10.1134/S000635091905004X ( Scopus, ВАК, article is available as 'Online First': http://link.springer.com/article/10.1134/S000635091905004X )

AU - Федоров, Даниил Александрович

AU - Фролова, Милена Юрьевна

AU - Красовская, Ирина Евгеньевна

AU - Кулева, Надежда Владимировна

N1 - Fedorov, D.A., Frolova, M.Y., Krasovskaya, I.E. et al. The Effects of Severe Hypobaric Hypoxia and Inhibition of Hypoxia-Inducible Factor-1 (HIF-1) on Biomarkers of Cardiac and Skeletal Muscle Injury in Rats. BIOPHYSICS 64, 808–811 (2019) doi:10.1134/S000635091905004X

PY - 2019

Y1 - 2019

N2 - The goal of the present study was to investigate the molecular mechanisms that underlie heart andskeletal muscle damage in male Wistar rats weighing 200–250 g in response to a 3-h exposure to 180 mm Hg(5% O2) in the model of severe hypobaric hypoxia. It has been demonstrated that the level of the cardiac biomarkertroponin I in the blood plasma of rats exposed to severe hypobaric hypoxia for 3 h increased significantlycompared to the control group, indicating myocardial injury. At the same time, the administration ofthe HIF-1α transcription factor inhibitor did not affect the plasma level of troponin I. In contrast, the releaseof the non-specific biomarker myoglobin into the bloodstream did not increase in response to hypoxia comparedto the control animals. In addition, 24 h after the exposure to severe hypobaric hypoxia the serum myoglobinlevel was significantly lower in animals administered with the HIF-1α inhibitor topotecan than in ratsthat did not receive topotecan. Therefore, it may be assumed that the inhibition of the HIF-1α transcriptionfactor 10 min before exposure to severe hypobaric hypoxia reduces skeletal muscle damage. The mechanismsthat affect the adaptation of heart and skeletal muscles to hypoxia are discussed.

AB - The goal of the present study was to investigate the molecular mechanisms that underlie heart andskeletal muscle damage in male Wistar rats weighing 200–250 g in response to a 3-h exposure to 180 mm Hg(5% O2) in the model of severe hypobaric hypoxia. It has been demonstrated that the level of the cardiac biomarkertroponin I in the blood plasma of rats exposed to severe hypobaric hypoxia for 3 h increased significantlycompared to the control group, indicating myocardial injury. At the same time, the administration ofthe HIF-1α transcription factor inhibitor did not affect the plasma level of troponin I. In contrast, the releaseof the non-specific biomarker myoglobin into the bloodstream did not increase in response to hypoxia comparedto the control animals. In addition, 24 h after the exposure to severe hypobaric hypoxia the serum myoglobinlevel was significantly lower in animals administered with the HIF-1α inhibitor topotecan than in ratsthat did not receive topotecan. Therefore, it may be assumed that the inhibition of the HIF-1α transcriptionfactor 10 min before exposure to severe hypobaric hypoxia reduces skeletal muscle damage. The mechanismsthat affect the adaptation of heart and skeletal muscles to hypoxia are discussed.

KW - hypoxia, myoglobin, troponin I, topotecan,hypoxia-inducible factor 1-alpha (HIF-1α), myocardium

KW - myoglobin

UR - http://link.springer.com/article/10.1134/S000635091905004X

M3 - Article

VL - 64

SP - 808

EP - 811

JO - Biophysics (Russian Federation)

JF - Biophysics (Russian Federation)

SN - 0006-3509

IS - 5

M1 - DOI 10.1134/S000635091905004X

ER -