Research output: Contribution to journal › Article › peer-review
Targeting brain tumors with mesenchymal stem cells in the experimental model of the orthotopic glioblastoma in rats. / Yudintceva, Natalia; Lomert, Ekaterina; Mikhailova, Natalia; Tolkunova, Elena; Agadzhanian, Nikol; Samochernych, Konstantin; Multhoff, Gabriele; Timin, Grigoriy; Ryzhov, Vyacheslav; Deriglazov, Vladimir; Mazur, Anton; Shevtsov, Maxim.
In: Biomedicines, Vol. 9, No. 11, 1592, 01.11.2021.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Targeting brain tumors with mesenchymal stem cells in the experimental model of the orthotopic glioblastoma in rats
AU - Yudintceva, Natalia
AU - Lomert, Ekaterina
AU - Mikhailova, Natalia
AU - Tolkunova, Elena
AU - Agadzhanian, Nikol
AU - Samochernych, Konstantin
AU - Multhoff, Gabriele
AU - Timin, Grigoriy
AU - Ryzhov, Vyacheslav
AU - Deriglazov, Vladimir
AU - Mazur, Anton
AU - Shevtsov, Maxim
N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/11/1
Y1 - 2021/11/1
N2 - Despite multimodal approaches for the treatment of multiforme glioblastoma (GBM) advances in outcome have been very modest indicating the necessity of novel diagnostic and therapeutic strategies. Currently, mesenchymal stem cells (MSCs) represent a promising platform for cell-based cancer therapies because of their tumor-tropism, low immunogenicity, easy accessibility, isolation procedure, and culturing. In the present study, we assessed the tumor-tropism and biodistribution of the superparamagnetic iron oxide nanoparticle (SPION)-labeled MSCs in the orthotopic model of C6 glioblastoma in Wistar rats. As shown in in vitro studies employing confocal microscopy, high-content quantitative image cytometer, and xCelligence system MSCs exhibit a high migratory capacity towards C6 glioblastoma cells. Intravenous administration of SPION-labeled MSCs in vivo resulted in intratumoral accumulation of the tagged cells in the tumor tissues that in turn significantly enhanced the contrast of the tumor when high-field magnetic resonance imaging was performed. Subsequent biodistribution studies employing highly sensitive nonlinear magnetic response measurements (NLR-M2 ) supported by histological analysis confirm the retention of MSCs in the glioblastoma. In conclusion, MSCs due to their tumor-tropism could be employed as a drug-delivery platform for future theranostic approaches.
AB - Despite multimodal approaches for the treatment of multiforme glioblastoma (GBM) advances in outcome have been very modest indicating the necessity of novel diagnostic and therapeutic strategies. Currently, mesenchymal stem cells (MSCs) represent a promising platform for cell-based cancer therapies because of their tumor-tropism, low immunogenicity, easy accessibility, isolation procedure, and culturing. In the present study, we assessed the tumor-tropism and biodistribution of the superparamagnetic iron oxide nanoparticle (SPION)-labeled MSCs in the orthotopic model of C6 glioblastoma in Wistar rats. As shown in in vitro studies employing confocal microscopy, high-content quantitative image cytometer, and xCelligence system MSCs exhibit a high migratory capacity towards C6 glioblastoma cells. Intravenous administration of SPION-labeled MSCs in vivo resulted in intratumoral accumulation of the tagged cells in the tumor tissues that in turn significantly enhanced the contrast of the tumor when high-field magnetic resonance imaging was performed. Subsequent biodistribution studies employing highly sensitive nonlinear magnetic response measurements (NLR-M2 ) supported by histological analysis confirm the retention of MSCs in the glioblastoma. In conclusion, MSCs due to their tumor-tropism could be employed as a drug-delivery platform for future theranostic approaches.
KW - Biodistribution
KW - C6 glioma
KW - Magnetic resonance imaging
KW - Mesenchymal stem cells
KW - Multiforme glioblastoma
KW - Nonlinear magnetic response
KW - Superparamagnetic iron oxide nanoparticles
KW - Targeted drug delivery
KW - IRON-OXIDE NANOPARTICLES
KW - biodistribution
KW - magnetic resonance imaging
KW - multiforme glioblastoma
KW - targeted drug delivery
KW - nonlinear magnetic response
KW - BIODISTRIBUTION
KW - THERAPY
KW - TROPISM
KW - mesenchymal stem cells
KW - superparamagnetic iron oxide nanoparticles
KW - GENERATION
UR - http://www.scopus.com/inward/record.url?scp=85118530373&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/893bab4e-2170-3505-a52d-8f4a6eaba899/
U2 - 10.3390/biomedicines9111592
DO - 10.3390/biomedicines9111592
M3 - Article
AN - SCOPUS:85118530373
VL - 9
JO - Biomedicines
JF - Biomedicines
SN - 2227-9059
IS - 11
M1 - 1592
ER -
ID: 90309512