Stabilization of DNA by sodium and magnesium ions during the synthesis of DNA-bridged gold nanoparticles

Petr A. Sokolov, Ruslan R. Ramazanov, Valeriy I. Rolich, Maria A. Popova, Vyacheslav E. Shalygin, Nina A. Kasyanenko

Research output: Contribution to journalArticlepeer-review

Abstract

Nanostructures synthesized using DNA-conjugated gold nanoparticles have a wide range of applications in the field of biosensorics. The stability of the DNA duplex plays a critical role as it determines the final geometry of these nanostructures. The main way to control DNA stability is to maintain a high ionic strength of the buffer solution; at the same time, high salt concentrations lead to an aggregation of nanoparticles. In this study, by means of the instrumentality of DNA-bridged seeds using tris(hydroxymethyl)aminomethane as a soft reducing agent the dumbbell-like gold nanoparticles up to 35 nm were synthesized with a high concentration of sodium ions of up to 100 mM and magnesium ions up to 1 mM. We also examined at the atomic level the details of the effect of the gold nanoparticle surface, as well as Na+ and Mg2+ ions, on the stability of nucleotide pairs located in close proximity to the grafting site.

Original languageEnglish
Article number045604
Number of pages10
JournalNanotechnology
Volume32
Issue number4
DOIs
StatePublished - 22 Jan 2021

Scopus subject areas

  • Mechanics of Materials
  • Mechanical Engineering
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Electrical and Electronic Engineering

Keywords

  • DNA
  • Dumbbells
  • Gold
  • Nanoparticles
  • Plasmonics
  • Salt
  • salt
  • UNIFORM
  • SIZES
  • ADSORPTION
  • CYTOSINE
  • gold
  • DENSITY
  • dumbbells
  • nanoparticles
  • plasmonics
  • GROWTH
  • SURFACE
  • NUCLEOBASES

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