TY - JOUR

T1 - Smart colloids containing ensembles of gold nanoparticles conjugated with kappa-carrageenan

AU - Gasilova, Ekaterina R.

AU - Aleksandrova, Galina P.

AU - Volchek, Boris Z.

AU - Vlasova, Elena N.

AU - Baigildin, Vadim A.

N1 - Export Date: 17 September 2019

PY - 2019

Y1 - 2019

N2 - We study colloids of nanobiocomposites (NBC) containing Au nanoparticles (NP) obtained by reduction of gold precursor with functional groups of κCAR. The AuNPs content was changed from 2.0 to 5.6 w/w %. Hydrolysis of κCAR during reduction of gold ions produced carboxylate groups supporting nanobioconjugation (FT-IR data) and electrostatic stabilization through increased negative ζ-potential. The self-assembly of AuNPs took place after storing NBC, as shown by UV/vis. extinction and transmission electron microscopy. Analysis of the structure-sensitive R g/R h ratios obtained by dynamic and static light scattering showed that the colloids have core-shell structure. The macromolecules in the shell exhibited polyelectrolyte expansion at [NaCl] and [KCl] < 0.02 M. Above 0.02 M, the difference between non-specific sodium and specific potassium ions on the shell thickness was registered. The temperature hysteresis of the hydrodynamic radius R h was similar to that of the pristine κCAR observed previously by optical rotation. Monitoring the equilibration of light scattering intensity at each mini-quenching step of the cooling run allowed further insight into the hysteresis origin. These colloids are interesting for sensing applications because of enhanced scattering of plasmonically coupled AuNPE. Their thermo- and ion-sensitivity can be useful in drug delivery.

AB - We study colloids of nanobiocomposites (NBC) containing Au nanoparticles (NP) obtained by reduction of gold precursor with functional groups of κCAR. The AuNPs content was changed from 2.0 to 5.6 w/w %. Hydrolysis of κCAR during reduction of gold ions produced carboxylate groups supporting nanobioconjugation (FT-IR data) and electrostatic stabilization through increased negative ζ-potential. The self-assembly of AuNPs took place after storing NBC, as shown by UV/vis. extinction and transmission electron microscopy. Analysis of the structure-sensitive R g/R h ratios obtained by dynamic and static light scattering showed that the colloids have core-shell structure. The macromolecules in the shell exhibited polyelectrolyte expansion at [NaCl] and [KCl] < 0.02 M. Above 0.02 M, the difference between non-specific sodium and specific potassium ions on the shell thickness was registered. The temperature hysteresis of the hydrodynamic radius R h was similar to that of the pristine κCAR observed previously by optical rotation. Monitoring the equilibration of light scattering intensity at each mini-quenching step of the cooling run allowed further insight into the hysteresis origin. These colloids are interesting for sensing applications because of enhanced scattering of plasmonically coupled AuNPE. Their thermo- and ion-sensitivity can be useful in drug delivery.

KW - Light scattering

KW - Nanobiocomposite colloids

KW - κ-Carrageenan

KW - TRANSITION

KW - DYNAMIC LIGHT-SCATTERING

KW - kappa-Carrageenan

KW - BEHAVIOR

KW - GREEN SYNTHESIS

KW - DEGRADATION

KW - SILVER NANOPARTICLES

UR - http://www.scopus.com/inward/record.url?scp=85068409960&partnerID=8YFLogxK

U2 - 10.1016/j.ijbiomac.2019.06.215

DO - 10.1016/j.ijbiomac.2019.06.215

M3 - Article

VL - 137

SP - 358

EP - 365

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -