Silicon ameliorates iron deficiency of cucumber in a pH-dependent manner

Nikolai P. Bityutskii, Kirill L. Yakkonen, Анастасия Игоревна Петрова, Kseniia A. Lukina, Alexey L. Shavarda

Research output

Abstract

Strategy I plants may respond to iron (Fe) deficiency by rhizosphere acidification. Here, the role of medium pH-values in silicon (Si)-induced mitigation Fe deficiency in Strategy I plants (Cucumis sativus) was investigated, particularly the metabolites regulated by a lack of Fe, using a target metabolomics approach. Plants were grown hydroponically, either with (+Fe) or in Fe-free (−Fe) nutrient solution, with (+Si) or without (−Si) a Si supply. The nutrient solution was adjusted to pH 5.0 or 6.0 and checked daily. Leaf metabolites potentially involved in Fe transport were determined. The typical Fe responses of cucumber (e.g., decrease in leaf chlorophyll, Fe imbalance) were more pronounced when plants were grown at pH 6.0 than 5.0, during long-term Fe deficiency (15 days). Major metabolites up-regulated by Fe deficiency and found in young leaf were succinic, citric and glutamic acids, respectively; their maximal concentrations occurred in Fe-starved plants grown at pH 6.0 without Si supply. Silicon (Si)-induced effects accompanied with alleviation chlorosis symptoms, were most distinct in plants grown at pH 6.0 for an extended period without Fe. Changes in abundance of metabolites specifically up-regulated by a lack of Fe may be manifested before any Si-induced changes in plant Fe content were apparent, suggesting that metabolite responses are highly sensitive to a Fe-dependent signal altered by Si treatments under Fe deficiency. The results indicate that Si supply was more evident when plants were more stressed by an increase in nutrient solution pH under Fe-limited conditions.

Original languageEnglish
Pages (from-to)364-373
Number of pages10
JournalJournal of Plant Physiology
Volume231
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

Cucumis sativus
Silicon
silicon
cucumbers
Iron
iron
metabolites
nutrient solutions
Succinates
Glutamates
Hypochromic Anemia
leaves
Rhizosphere
Metabolomics
metabolomics
Chlorophyll
succinic acid
chlorosis
glutamic acid
citric acid

Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Bityutskii, Nikolai P. ; Yakkonen, Kirill L. ; Петрова, Анастасия Игоревна ; Lukina, Kseniia A. ; Shavarda, Alexey L. . / Silicon ameliorates iron deficiency of cucumber in a pH-dependent manner. In: Journal of Plant Physiology. 2018 ; Vol. 231. pp. 364-373.
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title = "Silicon ameliorates iron deficiency of cucumber in a pH-dependent manner",
abstract = "Strategy I plants may respond to iron (Fe) deficiency by rhizosphere acidification. Here, the role of medium pH-values in silicon (Si)-induced mitigation Fe deficiency in Strategy I plants (Cucumis sativus) was investigated, particularly the metabolites regulated by a lack of Fe, using a target metabolomics approach. Plants were grown hydroponically, either with (+Fe) or in Fe-free (−Fe) nutrient solution, with (+Si) or without (−Si) a Si supply. The nutrient solution was adjusted to pH 5.0 or 6.0 and checked daily. Leaf metabolites potentially involved in Fe transport were determined. The typical Fe responses of cucumber (e.g., decrease in leaf chlorophyll, Fe imbalance) were more pronounced when plants were grown at pH 6.0 than 5.0, during long-term Fe deficiency (15 days). Major metabolites up-regulated by Fe deficiency and found in young leaf were succinic, citric and glutamic acids, respectively; their maximal concentrations occurred in Fe-starved plants grown at pH 6.0 without Si supply. Silicon (Si)-induced effects accompanied with alleviation chlorosis symptoms, were most distinct in plants grown at pH 6.0 for an extended period without Fe. Changes in abundance of metabolites specifically up-regulated by a lack of Fe may be manifested before any Si-induced changes in plant Fe content were apparent, suggesting that metabolite responses are highly sensitive to a Fe-dependent signal altered by Si treatments under Fe deficiency. The results indicate that Si supply was more evident when plants were more stressed by an increase in nutrient solution pH under Fe-limited conditions.",
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Silicon ameliorates iron deficiency of cucumber in a pH-dependent manner. / Bityutskii, Nikolai P.; Yakkonen, Kirill L.; Петрова, Анастасия Игоревна; Lukina, Kseniia A.; Shavarda, Alexey L. .

In: Journal of Plant Physiology, Vol. 231, 01.12.2018, p. 364-373.

Research output

TY - JOUR

T1 - Silicon ameliorates iron deficiency of cucumber in a pH-dependent manner

AU - Bityutskii, Nikolai P.

AU - Yakkonen, Kirill L.

AU - Петрова, Анастасия Игоревна

AU - Lukina, Kseniia A.

AU - Shavarda, Alexey L.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Strategy I plants may respond to iron (Fe) deficiency by rhizosphere acidification. Here, the role of medium pH-values in silicon (Si)-induced mitigation Fe deficiency in Strategy I plants (Cucumis sativus) was investigated, particularly the metabolites regulated by a lack of Fe, using a target metabolomics approach. Plants were grown hydroponically, either with (+Fe) or in Fe-free (−Fe) nutrient solution, with (+Si) or without (−Si) a Si supply. The nutrient solution was adjusted to pH 5.0 or 6.0 and checked daily. Leaf metabolites potentially involved in Fe transport were determined. The typical Fe responses of cucumber (e.g., decrease in leaf chlorophyll, Fe imbalance) were more pronounced when plants were grown at pH 6.0 than 5.0, during long-term Fe deficiency (15 days). Major metabolites up-regulated by Fe deficiency and found in young leaf were succinic, citric and glutamic acids, respectively; their maximal concentrations occurred in Fe-starved plants grown at pH 6.0 without Si supply. Silicon (Si)-induced effects accompanied with alleviation chlorosis symptoms, were most distinct in plants grown at pH 6.0 for an extended period without Fe. Changes in abundance of metabolites specifically up-regulated by a lack of Fe may be manifested before any Si-induced changes in plant Fe content were apparent, suggesting that metabolite responses are highly sensitive to a Fe-dependent signal altered by Si treatments under Fe deficiency. The results indicate that Si supply was more evident when plants were more stressed by an increase in nutrient solution pH under Fe-limited conditions.

AB - Strategy I plants may respond to iron (Fe) deficiency by rhizosphere acidification. Here, the role of medium pH-values in silicon (Si)-induced mitigation Fe deficiency in Strategy I plants (Cucumis sativus) was investigated, particularly the metabolites regulated by a lack of Fe, using a target metabolomics approach. Plants were grown hydroponically, either with (+Fe) or in Fe-free (−Fe) nutrient solution, with (+Si) or without (−Si) a Si supply. The nutrient solution was adjusted to pH 5.0 or 6.0 and checked daily. Leaf metabolites potentially involved in Fe transport were determined. The typical Fe responses of cucumber (e.g., decrease in leaf chlorophyll, Fe imbalance) were more pronounced when plants were grown at pH 6.0 than 5.0, during long-term Fe deficiency (15 days). Major metabolites up-regulated by Fe deficiency and found in young leaf were succinic, citric and glutamic acids, respectively; their maximal concentrations occurred in Fe-starved plants grown at pH 6.0 without Si supply. Silicon (Si)-induced effects accompanied with alleviation chlorosis symptoms, were most distinct in plants grown at pH 6.0 for an extended period without Fe. Changes in abundance of metabolites specifically up-regulated by a lack of Fe may be manifested before any Si-induced changes in plant Fe content were apparent, suggesting that metabolite responses are highly sensitive to a Fe-dependent signal altered by Si treatments under Fe deficiency. The results indicate that Si supply was more evident when plants were more stressed by an increase in nutrient solution pH under Fe-limited conditions.

KW - Cucumis sativus

KW - Iron deficiency

KW - Metabolites

KW - Nutrient solution

KW - Silicon

KW - pH values

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U2 - 10.1016/j.jplph.2018.10.017

DO - 10.1016/j.jplph.2018.10.017

M3 - Article

VL - 231

SP - 364

EP - 373

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

ER -