Significance and Regulation of Acids Production by Rock-Inhabited Fungi

Katerina V. Sazanova, Dmitry Yu. Vlasov, Natalia G. Osmolovskay, Sergei M. Schiparev, Alexey V. Rusakov

Research output

2 Citations (Scopus)

Abstract

According to the results of screening acidification activity of 24 species of micromycetes in vitro, 18 of them produced organic acids in artificial medium. The fungi from genera Penicillium and strains of Aspergillus niger have the most active production of organic acids. The ratio of acids formed by fungi on a liquid glucose-containing medium changes during ontogenesis: gluconic acid and acids of the Krebs cycle dominated in a log phase of growth; oxalic acid accumulated in a stationary phase while concentrations of other organic acids reduced. Oxalic acid is the main acid produced by fungi in a culture and apparently in a natural habitat. Formation of gluconate improves the efficiency of other carbon substrates consumption by fungi. Citric, succinic, malic, and fumaric acids are produced only at higher concentrations of carbohydrate and high ratio of C/N. Production of oxalic acid by micromycetes increases on the media containing only nitrate nitrogen. Zn in concentration up to 2 mM induces secretion of oxalic acid on nitrate media. Copper has stimulation effect on oxalic acid production only in very low concentration (25 µM). Presence of Zn in culture leads to formation of oxalate Zn crystals on the surface of mycelium. On ammonium-containing culture Zn and Cu does not stimulate oxalic acid production, but induce significant morphological changes.
Original languageEnglish
Title of host publicationBiogenic – Abiogenic Interactions in Natural and Anthropogenic Systems. Lecture Notes in Earth System Sciences
PublisherSpringer
Pages379-392
ISBN (Electronic)978-3-319-24987-2
ISBN (Print)978-3-319-24985-8
DOIs
Publication statusPublished - 2016

Publication series

NameLecture Notes in Earth System Sciences
PublisherSpringer International Publishing
ISSN (Print)2193-8571

Fingerprint

oxalic acid
rocks
fungi
acids
organic acids and salts
gluconic acid
fumaric acid
tricarboxylic acid cycle
nitrate nitrogen
malic acid
oxalates
succinic acid
Aspergillus niger
Penicillium
citric acid
carbon nitrogen ratio
acidification
mycelium
crystals
ontogeny

Cite this

Sazanova, K. V., Vlasov, D. Y., Osmolovskay, N. G., Schiparev, S. M., & Rusakov, A. V. (2016). Significance and Regulation of Acids Production by Rock-Inhabited Fungi. In Biogenic – Abiogenic Interactions in Natural and Anthropogenic Systems. Lecture Notes in Earth System Sciences (pp. 379-392). (Lecture Notes in Earth System Sciences). Springer. https://doi.org/10.1007/978-3-319-24987-2_29
Sazanova, Katerina V. ; Vlasov, Dmitry Yu. ; Osmolovskay, Natalia G. ; Schiparev, Sergei M. ; Rusakov, Alexey V. / Significance and Regulation of Acids Production by Rock-Inhabited Fungi. Biogenic – Abiogenic Interactions in Natural and Anthropogenic Systems. Lecture Notes in Earth System Sciences. Springer, 2016. pp. 379-392 (Lecture Notes in Earth System Sciences).
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Sazanova, KV, Vlasov, DY, Osmolovskay, NG, Schiparev, SM & Rusakov, AV 2016, Significance and Regulation of Acids Production by Rock-Inhabited Fungi. in Biogenic – Abiogenic Interactions in Natural and Anthropogenic Systems. Lecture Notes in Earth System Sciences. Lecture Notes in Earth System Sciences, Springer, pp. 379-392. https://doi.org/10.1007/978-3-319-24987-2_29

Significance and Regulation of Acids Production by Rock-Inhabited Fungi. / Sazanova, Katerina V.; Vlasov, Dmitry Yu.; Osmolovskay, Natalia G.; Schiparev, Sergei M.; Rusakov, Alexey V.

Biogenic – Abiogenic Interactions in Natural and Anthropogenic Systems. Lecture Notes in Earth System Sciences. Springer, 2016. p. 379-392 (Lecture Notes in Earth System Sciences).

Research output

TY - CHAP

T1 - Significance and Regulation of Acids Production by Rock-Inhabited Fungi

AU - Sazanova, Katerina V.

AU - Vlasov, Dmitry Yu.

AU - Osmolovskay, Natalia G.

AU - Schiparev, Sergei M.

AU - Rusakov, Alexey V.

PY - 2016

Y1 - 2016

N2 - According to the results of screening acidification activity of 24 species of micromycetes in vitro, 18 of them produced organic acids in artificial medium. The fungi from genera Penicillium and strains of Aspergillus niger have the most active production of organic acids. The ratio of acids formed by fungi on a liquid glucose-containing medium changes during ontogenesis: gluconic acid and acids of the Krebs cycle dominated in a log phase of growth; oxalic acid accumulated in a stationary phase while concentrations of other organic acids reduced. Oxalic acid is the main acid produced by fungi in a culture and apparently in a natural habitat. Formation of gluconate improves the efficiency of other carbon substrates consumption by fungi. Citric, succinic, malic, and fumaric acids are produced only at higher concentrations of carbohydrate and high ratio of C/N. Production of oxalic acid by micromycetes increases on the media containing only nitrate nitrogen. Zn in concentration up to 2 mM induces secretion of oxalic acid on nitrate media. Copper has stimulation effect on oxalic acid production only in very low concentration (25 µM). Presence of Zn in culture leads to formation of oxalate Zn crystals on the surface of mycelium. On ammonium-containing culture Zn and Cu does not stimulate oxalic acid production, but induce significant morphological changes.

AB - According to the results of screening acidification activity of 24 species of micromycetes in vitro, 18 of them produced organic acids in artificial medium. The fungi from genera Penicillium and strains of Aspergillus niger have the most active production of organic acids. The ratio of acids formed by fungi on a liquid glucose-containing medium changes during ontogenesis: gluconic acid and acids of the Krebs cycle dominated in a log phase of growth; oxalic acid accumulated in a stationary phase while concentrations of other organic acids reduced. Oxalic acid is the main acid produced by fungi in a culture and apparently in a natural habitat. Formation of gluconate improves the efficiency of other carbon substrates consumption by fungi. Citric, succinic, malic, and fumaric acids are produced only at higher concentrations of carbohydrate and high ratio of C/N. Production of oxalic acid by micromycetes increases on the media containing only nitrate nitrogen. Zn in concentration up to 2 mM induces secretion of oxalic acid on nitrate media. Copper has stimulation effect on oxalic acid production only in very low concentration (25 µM). Presence of Zn in culture leads to formation of oxalate Zn crystals on the surface of mycelium. On ammonium-containing culture Zn and Cu does not stimulate oxalic acid production, but induce significant morphological changes.

KW - Organic acids

KW - Fungi

KW - Rock substrate

KW - Oxalates

KW - Adaptation

KW - Trophic conditions

KW - Heavy metals

U2 - 10.1007/978-3-319-24987-2_29

DO - 10.1007/978-3-319-24987-2_29

M3 - Chapter

SN - 978-3-319-24985-8

T3 - Lecture Notes in Earth System Sciences

SP - 379

EP - 392

BT - Biogenic – Abiogenic Interactions in Natural and Anthropogenic Systems. Lecture Notes in Earth System Sciences

PB - Springer

ER -

Sazanova KV, Vlasov DY, Osmolovskay NG, Schiparev SM, Rusakov AV. Significance and Regulation of Acids Production by Rock-Inhabited Fungi. In Biogenic – Abiogenic Interactions in Natural and Anthropogenic Systems. Lecture Notes in Earth System Sciences. Springer. 2016. p. 379-392. (Lecture Notes in Earth System Sciences). https://doi.org/10.1007/978-3-319-24987-2_29