Self-assemble nanoparticles based on polypeptides containing C-terminal luminescent Pt-cysteine complex

E. G. Vlakh, E. V. Grachova, D. D. Zhukovsky, A. V. Hubina, A. S. Mikhailova, J. R. Shakirova, V. V. Sharoyko, S. P. Tunik, T. B. Tennikova

Research outputpeer-review

8 Citations (Scopus)

Abstract

The growing attention to the luminescent nanocarriers is strongly stimulated by their potential application as drug delivery systems and by the necessity to monitor their distribution in cells and tissues. In this communication we report on the synthesis of amphiphilic polypeptides bearing C-terminal phosphorescent label together with preparation of nanoparticles using the polypeptides obtained. The approach suggested is based on a unique and highly technological process where the new phosphorescent Pt-cysteine complex serves as initiator of the ring-opening polymerization of a-amino acid N-carboxyanhydrides to obtain the polypeptides bearing intact the platinum chromophore covalently bound to the polymer chain. It was established that the luminescent label retains unchanged its emission characteristics not only in the polypeptides but also in more complicated nanoaggregates such as the polymer derived amphiphilic block-copolymers and self-assembled nanoparticles. The phosphorescent nanoparticles display no cytotoxicity and hemolytic activity in the tested range of concentrations and easily internalize into living cells that makes possible in vivo cell visualization, including prospective application in time resolved imaging and drug delivery monitoring.

Original languageEnglish
Article number41991
Number of pages10
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 3 Feb 2017

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Cysteine
Bearings (structural)
Nanoparticles
Peptides
Labels
Polymers
Ring opening polymerization
Chromophores
Cytotoxicity
Platinum
Drug delivery
Block copolymers
Visualization
Cells
Tissue
Imaging techniques
Amino Acids
Monitoring
Communication

Cite this

Vlakh, E. G. ; Grachova, E. V. ; Zhukovsky, D. D. ; Hubina, A. V. ; Mikhailova, A. S. ; Shakirova, J. R. ; Sharoyko, V. V. ; Tunik, S. P. ; Tennikova, T. B. / Self-assemble nanoparticles based on polypeptides containing C-terminal luminescent Pt-cysteine complex. In: Scientific Reports. 2017 ; Vol. 7.
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abstract = "The growing attention to the luminescent nanocarriers is strongly stimulated by their potential application as drug delivery systems and by the necessity to monitor their distribution in cells and tissues. In this communication we report on the synthesis of amphiphilic polypeptides bearing C-terminal phosphorescent label together with preparation of nanoparticles using the polypeptides obtained. The approach suggested is based on a unique and highly technological process where the new phosphorescent Pt-cysteine complex serves as initiator of the ring-opening polymerization of a-amino acid N-carboxyanhydrides to obtain the polypeptides bearing intact the platinum chromophore covalently bound to the polymer chain. It was established that the luminescent label retains unchanged its emission characteristics not only in the polypeptides but also in more complicated nanoaggregates such as the polymer derived amphiphilic block-copolymers and self-assembled nanoparticles. The phosphorescent nanoparticles display no cytotoxicity and hemolytic activity in the tested range of concentrations and easily internalize into living cells that makes possible in vivo cell visualization, including prospective application in time resolved imaging and drug delivery monitoring.",
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Self-assemble nanoparticles based on polypeptides containing C-terminal luminescent Pt-cysteine complex. / Vlakh, E. G.; Grachova, E. V.; Zhukovsky, D. D.; Hubina, A. V.; Mikhailova, A. S.; Shakirova, J. R.; Sharoyko, V. V.; Tunik, S. P.; Tennikova, T. B.

In: Scientific Reports, Vol. 7, 41991, 03.02.2017.

Research outputpeer-review

TY - JOUR

T1 - Self-assemble nanoparticles based on polypeptides containing C-terminal luminescent Pt-cysteine complex

AU - Vlakh, E. G.

AU - Grachova, E. V.

AU - Zhukovsky, D. D.

AU - Hubina, A. V.

AU - Mikhailova, A. S.

AU - Shakirova, J. R.

AU - Sharoyko, V. V.

AU - Tunik, S. P.

AU - Tennikova, T. B.

PY - 2017/2/3

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N2 - The growing attention to the luminescent nanocarriers is strongly stimulated by their potential application as drug delivery systems and by the necessity to monitor their distribution in cells and tissues. In this communication we report on the synthesis of amphiphilic polypeptides bearing C-terminal phosphorescent label together with preparation of nanoparticles using the polypeptides obtained. The approach suggested is based on a unique and highly technological process where the new phosphorescent Pt-cysteine complex serves as initiator of the ring-opening polymerization of a-amino acid N-carboxyanhydrides to obtain the polypeptides bearing intact the platinum chromophore covalently bound to the polymer chain. It was established that the luminescent label retains unchanged its emission characteristics not only in the polypeptides but also in more complicated nanoaggregates such as the polymer derived amphiphilic block-copolymers and self-assembled nanoparticles. The phosphorescent nanoparticles display no cytotoxicity and hemolytic activity in the tested range of concentrations and easily internalize into living cells that makes possible in vivo cell visualization, including prospective application in time resolved imaging and drug delivery monitoring.

AB - The growing attention to the luminescent nanocarriers is strongly stimulated by their potential application as drug delivery systems and by the necessity to monitor their distribution in cells and tissues. In this communication we report on the synthesis of amphiphilic polypeptides bearing C-terminal phosphorescent label together with preparation of nanoparticles using the polypeptides obtained. The approach suggested is based on a unique and highly technological process where the new phosphorescent Pt-cysteine complex serves as initiator of the ring-opening polymerization of a-amino acid N-carboxyanhydrides to obtain the polypeptides bearing intact the platinum chromophore covalently bound to the polymer chain. It was established that the luminescent label retains unchanged its emission characteristics not only in the polypeptides but also in more complicated nanoaggregates such as the polymer derived amphiphilic block-copolymers and self-assembled nanoparticles. The phosphorescent nanoparticles display no cytotoxicity and hemolytic activity in the tested range of concentrations and easily internalize into living cells that makes possible in vivo cell visualization, including prospective application in time resolved imaging and drug delivery monitoring.

KW - CYCLOMETALATED PLATINUM(II) COMPLEXES

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KW - POLYMERIC NANOPARTICLES

KW - CANCER-THERAPY

KW - FLUORESCENT

KW - LIGANDS

KW - NANOSTRUCTURES

KW - VISUALIZATION

KW - CARRIERS

U2 - 10.1038/srep41991

DO - 10.1038/srep41991

M3 - статья

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JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

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