TY - JOUR

T1 - Psychrobacillus syltrankelensis sp. nov., a new species isolated from soil in the North Caucasus, Russia

AU - Романенко, Мария Николаевна

AU - Шиков, Антон Евгеньевич

AU - Шматов, Федор

AU - Савина, Юлия

AU - Белоусов, Михаил Владимирович

AU - Соловченко, Алексей

AU - Чивкунова, Ольга

AU - Савельев, Григорий Константинович

AU - Кузнецова, Ирина Г.

AU - Карлов, Денис

AU - Нижников, Антон Александрович

AU - Антонец, Кирилл Сергеевич

PY - 2026/6/2

Y1 - 2026/6/2

N2 - Members of the genus Psychrobacillus are widely distributed across diverse environments, and novel representatives continue to be identified. Here, we describe the strain nc5.1T, isolated from soil near Lake Syltran-Kel in the Kabardino-Balkarian Republic, Russia. Phylogenomic analysis indicated that P. glaciei PB01T was its closest relative, placing the isolate within the genus Psychrobacillus. The average nucleotide identity (84.3%) and digital DNA–DNA hybridization (31%) values compared with PB01T suggested that the isolate represents a novel species. The strain nc5.1T was a Gram-negative, motile, rod-shaped bacterium forming circular, smooth, beige-brown colonies. No morphologically detectable endospores were observed under sporulation-inducing conditions. Optimal growth conditions were pH 6.5, 0.5% NaCl, and 30°C; however, a minor but reproducible viable fraction persisted after exposure to 80°C. Biochemical profiling revealed that the strain assimilated 46 carbon sources spanning carbohydrates, amino acids, and organic acids and exhibited growth in the presence of aztreonam, nalidixic acid, and potassium tellurite. The dominant cellular fatty acids were C14:1 (52.8%), C14:0 (16.0%), and C16:0 (7.7%), which distinguished the isolate from P. glaciei. Genomic analysis identified biosynthetic gene clusters associated with the synthesis of iturin and a streptozotocin-like moiety, suggesting the capacity for biosynthesis of secondary metabolites with beneficial properties. MTT assays showed that culture supernatants did not reduce mammalian cell viability but, conversely, moderately stimulated the proliferation of PANC-1 cells. Together, the phenotypic, chemotaxonomic, and genomic data indicate that strain nc5.1T represents a novel species with the proposed name Psychrobacillus syltrankelensis sp. nov. The type strain is nc5.1T (=RCAM 07221).

AB - Members of the genus Psychrobacillus are widely distributed across diverse environments, and novel representatives continue to be identified. Here, we describe the strain nc5.1T, isolated from soil near Lake Syltran-Kel in the Kabardino-Balkarian Republic, Russia. Phylogenomic analysis indicated that P. glaciei PB01T was its closest relative, placing the isolate within the genus Psychrobacillus. The average nucleotide identity (84.3%) and digital DNA–DNA hybridization (31%) values compared with PB01T suggested that the isolate represents a novel species. The strain nc5.1T was a Gram-negative, motile, rod-shaped bacterium forming circular, smooth, beige-brown colonies. No morphologically detectable endospores were observed under sporulation-inducing conditions. Optimal growth conditions were pH 6.5, 0.5% NaCl, and 30°C; however, a minor but reproducible viable fraction persisted after exposure to 80°C. Biochemical profiling revealed that the strain assimilated 46 carbon sources spanning carbohydrates, amino acids, and organic acids and exhibited growth in the presence of aztreonam, nalidixic acid, and potassium tellurite. The dominant cellular fatty acids were C14:1 (52.8%), C14:0 (16.0%), and C16:0 (7.7%), which distinguished the isolate from P. glaciei. Genomic analysis identified biosynthetic gene clusters associated with the synthesis of iturin and a streptozotocin-like moiety, suggesting the capacity for biosynthesis of secondary metabolites with beneficial properties. MTT assays showed that culture supernatants did not reduce mammalian cell viability but, conversely, moderately stimulated the proliferation of PANC-1 cells. Together, the phenotypic, chemotaxonomic, and genomic data indicate that strain nc5.1T represents a novel species with the proposed name Psychrobacillus syltrankelensis sp. nov. The type strain is nc5.1T (=RCAM 07221).

KW - Bacillales/genetics

KW - Bacterial Typing Techniques

KW - DNA, Bacterial/genetics

KW - Fatty Acids/metabolism

KW - Genome, Bacterial

KW - Nucleic Acid Hybridization

KW - Phylogeny

KW - RNA, Ribosomal, 16S/genetics

KW - Russia

KW - Soil Microbiology

UR - https://journals.asm.org/doi/10.1128/spectrum.02919-25

UR - https://www.mendeley.com/catalogue/3bb642e5-0f42-3543-82c7-dc3bcbdf07b5/

U2 - 10.1128/spectrum.02919-25

DO - 10.1128/spectrum.02919-25

M3 - Article

C2 - 42003585

VL - 14

JO - Microbiology spectrum

JF - Microbiology spectrum

SN - 2165-0497

IS - 6

M1 - e0291925

ER -