Properties of stem cells isolated from subcutaneous and subepicardial adipose tissues

T.A. Krylova, O.A. Bystrova, A.A. Khudyakov, A.B. Malashicheva, O.M. Moiseeva, V.V. Zenin, M.G. Martynova

Research output

1 Citation (Scopus)

Abstract

Stem cells (SCs) vary in morphological, immunophenotypic, proliferative, and differentiation characteristics depending on their tissue source. Comparative analysis of their biological properties is essential for making an optimal SC choice for regenerative therapy. Using immunocytochemistry, flow cytometry, histochemistry, and RT-PCR, we have investigated SCs obtained from human subepicardial (SEC-AT) and subcutaneous (SC-AT) adipose tissues and cultured under similar conditions without any differentiation-promoting factors. The cultures were similar in having a high proportion of proliferating cells positive for nuclear antigen (PCNA). In both cultures, immunophenotyping has revealed high expression of mesenchymal stem-cell surface markers CD29, CD44, CD73, and CD105; low expression of CD31, CD34, and CD45; and variability in CD117, CD146, and CD309 expression. The only difference in the CD marker profile was the significantly lower expression of CD90 in the culture of SCs from SC-AT than from SEC-AT. Histoc
Original languageEnglish
Pages (from-to)277-282
JournalCell and Tissue Biology
Issue number4
DOIs
Publication statusPublished - 2014
Externally publishedYes

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