PRONOUNCED HYPERACTIVITY, COGNITIVE DYSFUNCTIONS AND BDNF DYSREGULATION IN DOPAMINE TRANSPORTER KNOCKOUT RATS

Damiana Leo, Ilya Sukhanov, Francesca Zoratto, Placido Illiano, Lucia Caffino, Fabrizio Sanna, Giulia Messa, Marco Emanuele, Alessandro Esposito, Maria Dorofeikova, Evgeny A Budygin, Liudmila Mus, Evgenia E Efimova, Marco Niello, Stefano Espinoza, Tatyana D Sotnikova, Marius C Hoener, Gianni Laviola, Fabio Fumagalli, Walter AdrianiRaul R Gainetdinov

Research output

16 Citations (Scopus)

Abstract

Dopamine (DA) controls many vital physiological functions and is critically involved in several neuropsychiatric disorders such as schizophrenia and attention deficit hyperactivity disorder (ADHD). The major function of the plasma membrane dopamine transporter (DAT) is the rapid uptake of released DA into presynaptic nerve terminals leading to control of both the extracellular levels of DA and the intracellular stores of DA. Here, we present a newly developed strain of rats (DAT-knockout, DAT-KO rats) in which the gene encoding the DAT has been disrupted by using zinc finger nuclease technology (ZFN). Male and female DAT-KO rats develop normally but weigh less than heterozygote and wild-type rats and demonstrate pronounced spontaneous locomotor hyperactivity. While striatal extracellular DA lifetime and concentrations are significantly increased, the total tissue content of DA is markedly decreased demonstrating the key role of DAT in the control of DA neurotransmission. Hyperactivity of DAT-KO rats can be counteracted by amphetamine, methylphenidate, the partial Trace Amine-Associated Receptor 1 (TAAR1) agonist RO5203648 and haloperidol. DAT-KO rats also demonstrate a deficit in working memory and sensorimotor gating tests, less propensity to develop obsessive behaviors and show strong dysregulation in frontostriatal BDNF function. DAT-KO rats could provide a novel translational model for human diseases involving aberrant DA function and/or mutations affecting the DAT or related regulatory mechanisms.SIGNIFICANCE STATEMENTHere, we present a newly developed strain of rats in which the gene encoding the dopamine transporter (DAT) has been disrupted (DAT-KO rats). DAT-KO rats display functional hyperdopaminergia accompanied with pronounced spontaneous locomotor hyperactivity. Hyperactivity of DAT-KO rats can be counteracted by amphetamine, methylphenidate, and a few other compounds exerting inhibitory action on dopamine-dependent hyperactivity. DAT-KO rats also demonstrate cognitive deficits in working memory and sensorimotor gating tests, less propensity to develop compulsive behaviors and strong dysregulation in frontostriatal BDNF function. These observations highlight the key role of the DAT in the control of brain dopaminergic transmission. DAT-KO rats could provide a novel translational model for human diseases involving aberrant dopamine functions.

Original languageEnglish
JournalJournal of Neuroscience
Volume38
Issue number3
DOIs
Publication statusE-pub ahead of print - 2018

Fingerprint

Dopamine Plasma Membrane Transport Proteins
Brain-Derived Neurotrophic Factor
Dopamine
Sensory Gating
Cognitive Dysfunction
Methylphenidate
Amphetamine
Short-Term Memory
Compulsive Behavior
Obsessive Behavior
Corpus Striatum
Membrane Transport Proteins
Zinc Fingers
Presynaptic Terminals
Haloperidol
Attention Deficit Disorder with Hyperactivity

Cite this

Leo, Damiana ; Sukhanov, Ilya ; Zoratto, Francesca ; Illiano, Placido ; Caffino, Lucia ; Sanna, Fabrizio ; Messa, Giulia ; Emanuele, Marco ; Esposito, Alessandro ; Dorofeikova, Maria ; Budygin, Evgeny A ; Mus, Liudmila ; Efimova, Evgenia E ; Niello, Marco ; Espinoza, Stefano ; Sotnikova, Tatyana D ; Hoener, Marius C ; Laviola, Gianni ; Fumagalli, Fabio ; Adriani, Walter ; Gainetdinov, Raul R. / PRONOUNCED HYPERACTIVITY, COGNITIVE DYSFUNCTIONS AND BDNF DYSREGULATION IN DOPAMINE TRANSPORTER KNOCKOUT RATS. In: Journal of Neuroscience. 2018 ; Vol. 38, No. 3.
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abstract = "Dopamine (DA) controls many vital physiological functions and is critically involved in several neuropsychiatric disorders such as schizophrenia and attention deficit hyperactivity disorder (ADHD). The major function of the plasma membrane dopamine transporter (DAT) is the rapid uptake of released DA into presynaptic nerve terminals leading to control of both the extracellular levels of DA and the intracellular stores of DA. Here, we present a newly developed strain of rats (DAT-knockout, DAT-KO rats) in which the gene encoding the DAT has been disrupted by using zinc finger nuclease technology (ZFN). Male and female DAT-KO rats develop normally but weigh less than heterozygote and wild-type rats and demonstrate pronounced spontaneous locomotor hyperactivity. While striatal extracellular DA lifetime and concentrations are significantly increased, the total tissue content of DA is markedly decreased demonstrating the key role of DAT in the control of DA neurotransmission. Hyperactivity of DAT-KO rats can be counteracted by amphetamine, methylphenidate, the partial Trace Amine-Associated Receptor 1 (TAAR1) agonist RO5203648 and haloperidol. DAT-KO rats also demonstrate a deficit in working memory and sensorimotor gating tests, less propensity to develop obsessive behaviors and show strong dysregulation in frontostriatal BDNF function. DAT-KO rats could provide a novel translational model for human diseases involving aberrant DA function and/or mutations affecting the DAT or related regulatory mechanisms.SIGNIFICANCE STATEMENTHere, we present a newly developed strain of rats in which the gene encoding the dopamine transporter (DAT) has been disrupted (DAT-KO rats). DAT-KO rats display functional hyperdopaminergia accompanied with pronounced spontaneous locomotor hyperactivity. Hyperactivity of DAT-KO rats can be counteracted by amphetamine, methylphenidate, and a few other compounds exerting inhibitory action on dopamine-dependent hyperactivity. DAT-KO rats also demonstrate cognitive deficits in working memory and sensorimotor gating tests, less propensity to develop compulsive behaviors and strong dysregulation in frontostriatal BDNF function. These observations highlight the key role of the DAT in the control of brain dopaminergic transmission. DAT-KO rats could provide a novel translational model for human diseases involving aberrant dopamine functions.",
keywords = "Journal Article",
author = "Damiana Leo and Ilya Sukhanov and Francesca Zoratto and Placido Illiano and Lucia Caffino and Fabrizio Sanna and Giulia Messa and Marco Emanuele and Alessandro Esposito and Maria Dorofeikova and Budygin, {Evgeny A} and Liudmila Mus and Efimova, {Evgenia E} and Marco Niello and Stefano Espinoza and Sotnikova, {Tatyana D} and Hoener, {Marius C} and Gianni Laviola and Fabio Fumagalli and Walter Adriani and Gainetdinov, {Raul R}",
note = "Copyright {\circledC} 2018 the authors.",
year = "2018",
doi = "10.1523/JNEUROSCI.1931-17.2018",
language = "English",
volume = "38",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "3",

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Leo, D, Sukhanov, I, Zoratto, F, Illiano, P, Caffino, L, Sanna, F, Messa, G, Emanuele, M, Esposito, A, Dorofeikova, M, Budygin, EA, Mus, L, Efimova, EE, Niello, M, Espinoza, S, Sotnikova, TD, Hoener, MC, Laviola, G, Fumagalli, F, Adriani, W & Gainetdinov, RR 2018, 'PRONOUNCED HYPERACTIVITY, COGNITIVE DYSFUNCTIONS AND BDNF DYSREGULATION IN DOPAMINE TRANSPORTER KNOCKOUT RATS', Journal of Neuroscience, vol. 38, no. 3. https://doi.org/10.1523/JNEUROSCI.1931-17.2018

PRONOUNCED HYPERACTIVITY, COGNITIVE DYSFUNCTIONS AND BDNF DYSREGULATION IN DOPAMINE TRANSPORTER KNOCKOUT RATS. / Leo, Damiana; Sukhanov, Ilya; Zoratto, Francesca; Illiano, Placido; Caffino, Lucia; Sanna, Fabrizio; Messa, Giulia; Emanuele, Marco; Esposito, Alessandro; Dorofeikova, Maria; Budygin, Evgeny A; Mus, Liudmila; Efimova, Evgenia E; Niello, Marco; Espinoza, Stefano; Sotnikova, Tatyana D; Hoener, Marius C; Laviola, Gianni; Fumagalli, Fabio; Adriani, Walter; Gainetdinov, Raul R.

In: Journal of Neuroscience, Vol. 38, No. 3, 2018.

Research output

TY - JOUR

T1 - PRONOUNCED HYPERACTIVITY, COGNITIVE DYSFUNCTIONS AND BDNF DYSREGULATION IN DOPAMINE TRANSPORTER KNOCKOUT RATS

AU - Leo, Damiana

AU - Sukhanov, Ilya

AU - Zoratto, Francesca

AU - Illiano, Placido

AU - Caffino, Lucia

AU - Sanna, Fabrizio

AU - Messa, Giulia

AU - Emanuele, Marco

AU - Esposito, Alessandro

AU - Dorofeikova, Maria

AU - Budygin, Evgeny A

AU - Mus, Liudmila

AU - Efimova, Evgenia E

AU - Niello, Marco

AU - Espinoza, Stefano

AU - Sotnikova, Tatyana D

AU - Hoener, Marius C

AU - Laviola, Gianni

AU - Fumagalli, Fabio

AU - Adriani, Walter

AU - Gainetdinov, Raul R

N1 - Copyright © 2018 the authors.

PY - 2018

Y1 - 2018

N2 - Dopamine (DA) controls many vital physiological functions and is critically involved in several neuropsychiatric disorders such as schizophrenia and attention deficit hyperactivity disorder (ADHD). The major function of the plasma membrane dopamine transporter (DAT) is the rapid uptake of released DA into presynaptic nerve terminals leading to control of both the extracellular levels of DA and the intracellular stores of DA. Here, we present a newly developed strain of rats (DAT-knockout, DAT-KO rats) in which the gene encoding the DAT has been disrupted by using zinc finger nuclease technology (ZFN). Male and female DAT-KO rats develop normally but weigh less than heterozygote and wild-type rats and demonstrate pronounced spontaneous locomotor hyperactivity. While striatal extracellular DA lifetime and concentrations are significantly increased, the total tissue content of DA is markedly decreased demonstrating the key role of DAT in the control of DA neurotransmission. Hyperactivity of DAT-KO rats can be counteracted by amphetamine, methylphenidate, the partial Trace Amine-Associated Receptor 1 (TAAR1) agonist RO5203648 and haloperidol. DAT-KO rats also demonstrate a deficit in working memory and sensorimotor gating tests, less propensity to develop obsessive behaviors and show strong dysregulation in frontostriatal BDNF function. DAT-KO rats could provide a novel translational model for human diseases involving aberrant DA function and/or mutations affecting the DAT or related regulatory mechanisms.SIGNIFICANCE STATEMENTHere, we present a newly developed strain of rats in which the gene encoding the dopamine transporter (DAT) has been disrupted (DAT-KO rats). DAT-KO rats display functional hyperdopaminergia accompanied with pronounced spontaneous locomotor hyperactivity. Hyperactivity of DAT-KO rats can be counteracted by amphetamine, methylphenidate, and a few other compounds exerting inhibitory action on dopamine-dependent hyperactivity. DAT-KO rats also demonstrate cognitive deficits in working memory and sensorimotor gating tests, less propensity to develop compulsive behaviors and strong dysregulation in frontostriatal BDNF function. These observations highlight the key role of the DAT in the control of brain dopaminergic transmission. DAT-KO rats could provide a novel translational model for human diseases involving aberrant dopamine functions.

AB - Dopamine (DA) controls many vital physiological functions and is critically involved in several neuropsychiatric disorders such as schizophrenia and attention deficit hyperactivity disorder (ADHD). The major function of the plasma membrane dopamine transporter (DAT) is the rapid uptake of released DA into presynaptic nerve terminals leading to control of both the extracellular levels of DA and the intracellular stores of DA. Here, we present a newly developed strain of rats (DAT-knockout, DAT-KO rats) in which the gene encoding the DAT has been disrupted by using zinc finger nuclease technology (ZFN). Male and female DAT-KO rats develop normally but weigh less than heterozygote and wild-type rats and demonstrate pronounced spontaneous locomotor hyperactivity. While striatal extracellular DA lifetime and concentrations are significantly increased, the total tissue content of DA is markedly decreased demonstrating the key role of DAT in the control of DA neurotransmission. Hyperactivity of DAT-KO rats can be counteracted by amphetamine, methylphenidate, the partial Trace Amine-Associated Receptor 1 (TAAR1) agonist RO5203648 and haloperidol. DAT-KO rats also demonstrate a deficit in working memory and sensorimotor gating tests, less propensity to develop obsessive behaviors and show strong dysregulation in frontostriatal BDNF function. DAT-KO rats could provide a novel translational model for human diseases involving aberrant DA function and/or mutations affecting the DAT or related regulatory mechanisms.SIGNIFICANCE STATEMENTHere, we present a newly developed strain of rats in which the gene encoding the dopamine transporter (DAT) has been disrupted (DAT-KO rats). DAT-KO rats display functional hyperdopaminergia accompanied with pronounced spontaneous locomotor hyperactivity. Hyperactivity of DAT-KO rats can be counteracted by amphetamine, methylphenidate, and a few other compounds exerting inhibitory action on dopamine-dependent hyperactivity. DAT-KO rats also demonstrate cognitive deficits in working memory and sensorimotor gating tests, less propensity to develop compulsive behaviors and strong dysregulation in frontostriatal BDNF function. These observations highlight the key role of the DAT in the control of brain dopaminergic transmission. DAT-KO rats could provide a novel translational model for human diseases involving aberrant dopamine functions.

KW - Journal Article

U2 - 10.1523/JNEUROSCI.1931-17.2018

DO - 10.1523/JNEUROSCI.1931-17.2018

M3 - Article

C2 - 29348190

VL - 38

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 3

ER -