Profiling of Seed Proteome in Pea (Pisum sativum L.) Lines Characterized with High and Low Responsivity to Combined Inoculation with Nodule Bacteria and Arbuscular Mycorrhizal Fungi

Tatiana Mamontova, Alexey M. Afonin, Christian Ihling, Alena Soboleva, Elena Lukasheva, Anton S. Sulima, Oksana Y. Shtark, Gulnara A. Akhtemova, Maria N. Povydysh, Andrea Sinz, Andrej Frolov, Vladimir A. Zhukov, Igor A. Tikhonovich

Research output

1 Citation (Scopus)

Abstract

Legume crops represent the major source of food protein and contribute to human nutrition and animal feeding. An essential improvement of their productivity can be achieved by symbiosis with beneficial soil microorganisms—rhizobia (Rh) and arbuscular mycorrhizal (AM) fungi. The efficiency of these interactions depends on plant genotype. Recently, we have shown that, after simultaneous inoculation with Rh and AM, the productivity gain of pea (Pisum sativum L) line K-8274, characterized by high efficiency of interaction with soil microorganisms (EIBSM), was higher in comparison to a low-EIBSM line K-3358. However, the molecular mechanisms behind this effect are still uncharacterized. Therefore, here, we address the alterations in pea seed proteome, underlying the symbiosis-related productivity gain, and identify 111 differentially expressed proteins in the two lines. The high-EIBSM line K-8274 responded to inoculation by prolongation of seed maturation, manifested by up-regulation of proteins involved in cellular respiration, protein biosynthesis, and down-regulation of late-embryogenesis abundant (LEA) proteins. In contrast, the low-EIBSM line K-3358 demonstrated lower levels of the proteins, related to cell metabolism. Thus, we propose that the EIBSM trait is linked to prolongation of seed filling that needs to be taken into account in pulse crop breeding programs. The raw data have been deposited to the ProteomeXchange with identifier PXD013479.
Original languageEnglish
Article number1603
Number of pages27
JournalMolecules
Volume24
Issue number8
DOIs
Publication statusPublished - 2 Apr 2019

Cite this

Mamontova, Tatiana ; Afonin, Alexey M. ; Ihling, Christian ; Soboleva, Alena ; Lukasheva, Elena ; Sulima, Anton S. ; Shtark, Oksana Y. ; Akhtemova, Gulnara A. ; Povydysh, Maria N. ; Sinz, Andrea ; Frolov, Andrej ; Zhukov, Vladimir A. ; Tikhonovich, Igor A. / Profiling of Seed Proteome in Pea (Pisum sativum L.) Lines Characterized with High and Low Responsivity to Combined Inoculation with Nodule Bacteria and Arbuscular Mycorrhizal Fungi. In: Molecules. 2019 ; Vol. 24, No. 8.
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title = "Profiling of Seed Proteome in Pea (Pisum sativum L.) Lines Characterized with High and Low Responsivity to Combined Inoculation with Nodule Bacteria and Arbuscular Mycorrhizal Fungi",
abstract = "Legume crops represent the major source of food protein and contribute to human nutrition and animal feeding. An essential improvement of their productivity can be achieved by symbiosis with beneficial soil microorganisms—rhizobia (Rh) and arbuscular mycorrhizal (AM) fungi. The efficiency of these interactions depends on plant genotype. Recently, we have shown that, after simultaneous inoculation with Rh and AM, the productivity gain of pea (Pisum sativum L) line K-8274, characterized by high efficiency of interaction with soil microorganisms (EIBSM), was higher in comparison to a low-EIBSM line K-3358. However, the molecular mechanisms behind this effect are still uncharacterized. Therefore, here, we address the alterations in pea seed proteome, underlying the symbiosis-related productivity gain, and identify 111 differentially expressed proteins in the two lines. The high-EIBSM line K-8274 responded to inoculation by prolongation of seed maturation, manifested by up-regulation of proteins involved in cellular respiration, protein biosynthesis, and down-regulation of late-embryogenesis abundant (LEA) proteins. In contrast, the low-EIBSM line K-3358 demonstrated lower levels of the proteins, related to cell metabolism. Thus, we propose that the EIBSM trait is linked to prolongation of seed filling that needs to be taken into account in pulse crop breeding programs. The raw data have been deposited to the ProteomeXchange with identifier PXD013479.",
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author = "Tatiana Mamontova and Afonin, {Alexey M.} and Christian Ihling and Alena Soboleva and Elena Lukasheva and Sulima, {Anton S.} and Shtark, {Oksana Y.} and Akhtemova, {Gulnara A.} and Povydysh, {Maria N.} and Andrea Sinz and Andrej Frolov and Zhukov, {Vladimir A.} and Tikhonovich, {Igor A.}",
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Profiling of Seed Proteome in Pea (Pisum sativum L.) Lines Characterized with High and Low Responsivity to Combined Inoculation with Nodule Bacteria and Arbuscular Mycorrhizal Fungi. / Mamontova, Tatiana; Afonin, Alexey M.; Ihling, Christian; Soboleva, Alena; Lukasheva, Elena; Sulima, Anton S.; Shtark, Oksana Y.; Akhtemova, Gulnara A.; Povydysh, Maria N.; Sinz, Andrea; Frolov, Andrej; Zhukov, Vladimir A.; Tikhonovich, Igor A.

In: Molecules, Vol. 24, No. 8, 1603, 02.04.2019.

Research output

TY - JOUR

T1 - Profiling of Seed Proteome in Pea (Pisum sativum L.) Lines Characterized with High and Low Responsivity to Combined Inoculation with Nodule Bacteria and Arbuscular Mycorrhizal Fungi

AU - Mamontova, Tatiana

AU - Afonin, Alexey M.

AU - Ihling, Christian

AU - Soboleva, Alena

AU - Lukasheva, Elena

AU - Sulima, Anton S.

AU - Shtark, Oksana Y.

AU - Akhtemova, Gulnara A.

AU - Povydysh, Maria N.

AU - Sinz, Andrea

AU - Frolov, Andrej

AU - Zhukov, Vladimir A.

AU - Tikhonovich, Igor A.

PY - 2019/4/2

Y1 - 2019/4/2

N2 - Legume crops represent the major source of food protein and contribute to human nutrition and animal feeding. An essential improvement of their productivity can be achieved by symbiosis with beneficial soil microorganisms—rhizobia (Rh) and arbuscular mycorrhizal (AM) fungi. The efficiency of these interactions depends on plant genotype. Recently, we have shown that, after simultaneous inoculation with Rh and AM, the productivity gain of pea (Pisum sativum L) line K-8274, characterized by high efficiency of interaction with soil microorganisms (EIBSM), was higher in comparison to a low-EIBSM line K-3358. However, the molecular mechanisms behind this effect are still uncharacterized. Therefore, here, we address the alterations in pea seed proteome, underlying the symbiosis-related productivity gain, and identify 111 differentially expressed proteins in the two lines. The high-EIBSM line K-8274 responded to inoculation by prolongation of seed maturation, manifested by up-regulation of proteins involved in cellular respiration, protein biosynthesis, and down-regulation of late-embryogenesis abundant (LEA) proteins. In contrast, the low-EIBSM line K-3358 demonstrated lower levels of the proteins, related to cell metabolism. Thus, we propose that the EIBSM trait is linked to prolongation of seed filling that needs to be taken into account in pulse crop breeding programs. The raw data have been deposited to the ProteomeXchange with identifier PXD013479.

AB - Legume crops represent the major source of food protein and contribute to human nutrition and animal feeding. An essential improvement of their productivity can be achieved by symbiosis with beneficial soil microorganisms—rhizobia (Rh) and arbuscular mycorrhizal (AM) fungi. The efficiency of these interactions depends on plant genotype. Recently, we have shown that, after simultaneous inoculation with Rh and AM, the productivity gain of pea (Pisum sativum L) line K-8274, characterized by high efficiency of interaction with soil microorganisms (EIBSM), was higher in comparison to a low-EIBSM line K-3358. However, the molecular mechanisms behind this effect are still uncharacterized. Therefore, here, we address the alterations in pea seed proteome, underlying the symbiosis-related productivity gain, and identify 111 differentially expressed proteins in the two lines. The high-EIBSM line K-8274 responded to inoculation by prolongation of seed maturation, manifested by up-regulation of proteins involved in cellular respiration, protein biosynthesis, and down-regulation of late-embryogenesis abundant (LEA) proteins. In contrast, the low-EIBSM line K-3358 demonstrated lower levels of the proteins, related to cell metabolism. Thus, we propose that the EIBSM trait is linked to prolongation of seed filling that needs to be taken into account in pulse crop breeding programs. The raw data have been deposited to the ProteomeXchange with identifier PXD013479.

KW - )

KW - EMBRYOGENESIS

KW - EXPRESSION

KW - GENOME

KW - GLYCATION

KW - HEAT-SHOCK-PROTEIN

KW - LC-MS

KW - METABOLOME

KW - PLANT NUTRITION

KW - PLASMA-PROTEINS

KW - REVEALS

KW - SYSTEMS

KW - arbuscular mycorrhiza

KW - beneficial soil microorganisms

KW - combined inoculation

KW - inoculation efficiency

KW - pea (Pisum sativum L

KW - proteomics

KW - rhizobial symbiosis

KW - seed proteome

U2 - 10.3390/molecules24081603

DO - 10.3390/molecules24081603

M3 - Article

VL - 24

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 8

M1 - 1603

ER -