Polylactic acid nanoparticles influence on immunogenicity of the protein bound with them

D. S. Polyakov, O. I. Antimonova, R. G. Sakhabeev, N. A. Grudinina, A. E. Khodova, E. S. Sinitsyna, V. A. Korzhikov-Vlakh, T. B. Tennikova, M. M. Shavlovsky

Research output

Abstract

We investigated immunogenic properties of proteins bound with nanoparticles. A process for producing spherical nanoparticles having size of 20 microns by polymerization of lactic acid and an optimal method of nanoparticle surface activation were described. Activated nanoparticles were used for covalent binding of model fusion protein comprising sequences of human beta-2 microglobulin and green fluorescent protein. It is shown that the nanoparticles were able to bind 3 micrograms of the protein per 1 mg of the polymer. According to the results of confocal microscopy and electrophoresis the protein is firmly adsorbed on the surface of the granules. F1 (CBA x C57BL) mice were subjected to intraperitoneal immunization with fusion protein modified nanoparticles and equivalent mixture of unmodified nanoparticles and unbound fusion protein. Blood was taken at 2 weeks after three-time intraperitoneal immunization. Antibody level to model protein was determined in mouse sera using enzyme-linked immunosorbent assay. Each of experimental and control groups comprised 39 animals. The validity of the results was evaluated using the Mann-Whitney test. It is shown that the average antibody level in the control group was 1.8 times greater than that in the experimental group. The diffe rence was significant (p < 0.004). We discuss the significance of the results in terms of development traps capable to bind virus particles in blood and to provide immune response.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalRussian Journal of Infection and Immunity
Volume7
Issue number2
DOIs
Publication statusPublished - 1 Apr 2017

Fingerprint

Nanoparticles
Proteins
Immunization
beta 2-Microglobulin
Control Groups
Antibodies
Green Fluorescent Proteins
poly(lactic acid)
Inbred C57BL Mouse
Reproducibility of Results
Confocal Microscopy
Polymerization
Virion
Electrophoresis
Lactic Acid
Polymers
Enzyme-Linked Immunosorbent Assay
Serum

Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Polyakov, D. S., Antimonova, O. I., Sakhabeev, R. G., Grudinina, N. A., Khodova, A. E., Sinitsyna, E. S., ... Shavlovsky, M. M. (2017). Polylactic acid nanoparticles influence on immunogenicity of the protein bound with them. Russian Journal of Infection and Immunity, 7(2), 123-129. https://doi.org/10.15789/2220-7619-2017-2-123-129
Polyakov, D. S. ; Antimonova, O. I. ; Sakhabeev, R. G. ; Grudinina, N. A. ; Khodova, A. E. ; Sinitsyna, E. S. ; Korzhikov-Vlakh, V. A. ; Tennikova, T. B. ; Shavlovsky, M. M. / Polylactic acid nanoparticles influence on immunogenicity of the protein bound with them. In: Russian Journal of Infection and Immunity. 2017 ; Vol. 7, No. 2. pp. 123-129.
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Polylactic acid nanoparticles influence on immunogenicity of the protein bound with them. / Polyakov, D. S.; Antimonova, O. I.; Sakhabeev, R. G.; Grudinina, N. A.; Khodova, A. E.; Sinitsyna, E. S.; Korzhikov-Vlakh, V. A.; Tennikova, T. B.; Shavlovsky, M. M.

In: Russian Journal of Infection and Immunity, Vol. 7, No. 2, 01.04.2017, p. 123-129.

Research output

TY - JOUR

T1 - Polylactic acid nanoparticles influence on immunogenicity of the protein bound with them

AU - Polyakov, D. S.

AU - Antimonova, O. I.

AU - Sakhabeev, R. G.

AU - Grudinina, N. A.

AU - Khodova, A. E.

AU - Sinitsyna, E. S.

AU - Korzhikov-Vlakh, V. A.

AU - Tennikova, T. B.

AU - Shavlovsky, M. M.

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Y1 - 2017/4/1

N2 - We investigated immunogenic properties of proteins bound with nanoparticles. A process for producing spherical nanoparticles having size of 20 microns by polymerization of lactic acid and an optimal method of nanoparticle surface activation were described. Activated nanoparticles were used for covalent binding of model fusion protein comprising sequences of human beta-2 microglobulin and green fluorescent protein. It is shown that the nanoparticles were able to bind 3 micrograms of the protein per 1 mg of the polymer. According to the results of confocal microscopy and electrophoresis the protein is firmly adsorbed on the surface of the granules. F1 (CBA x C57BL) mice were subjected to intraperitoneal immunization with fusion protein modified nanoparticles and equivalent mixture of unmodified nanoparticles and unbound fusion protein. Blood was taken at 2 weeks after three-time intraperitoneal immunization. Antibody level to model protein was determined in mouse sera using enzyme-linked immunosorbent assay. Each of experimental and control groups comprised 39 animals. The validity of the results was evaluated using the Mann-Whitney test. It is shown that the average antibody level in the control group was 1.8 times greater than that in the experimental group. The diffe rence was significant (p < 0.004). We discuss the significance of the results in terms of development traps capable to bind virus particles in blood and to provide immune response.

AB - We investigated immunogenic properties of proteins bound with nanoparticles. A process for producing spherical nanoparticles having size of 20 microns by polymerization of lactic acid and an optimal method of nanoparticle surface activation were described. Activated nanoparticles were used for covalent binding of model fusion protein comprising sequences of human beta-2 microglobulin and green fluorescent protein. It is shown that the nanoparticles were able to bind 3 micrograms of the protein per 1 mg of the polymer. According to the results of confocal microscopy and electrophoresis the protein is firmly adsorbed on the surface of the granules. F1 (CBA x C57BL) mice were subjected to intraperitoneal immunization with fusion protein modified nanoparticles and equivalent mixture of unmodified nanoparticles and unbound fusion protein. Blood was taken at 2 weeks after three-time intraperitoneal immunization. Antibody level to model protein was determined in mouse sera using enzyme-linked immunosorbent assay. Each of experimental and control groups comprised 39 animals. The validity of the results was evaluated using the Mann-Whitney test. It is shown that the average antibody level in the control group was 1.8 times greater than that in the experimental group. The diffe rence was significant (p < 0.004). We discuss the significance of the results in terms of development traps capable to bind virus particles in blood and to provide immune response.

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