Research output: Contribution to journal › Article › peer-review
Phyllobacterium zundukense sp. Nov., a novel species of rhizobia isolated from root nodules of the legume species Oxytropis triphylla (Pall.) Pers. / Safronova, Vera I.; Sazanova, Anna L.; Kuznetsova, Irina G.; Belimov, Andrey A.; Andronov, Evgeny E.; Chirak, Elizaveta R.; Popova, Janna P.; Verkhozina, Alla V.; Willems, Anne; Tikhonovich, Igor A.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 5, 002722, 01.05.2018, p. 1644-1651.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Phyllobacterium zundukense sp. Nov., a novel species of rhizobia isolated from root nodules of the legume species Oxytropis triphylla (Pall.) Pers
AU - Safronova, Vera I.
AU - Sazanova, Anna L.
AU - Kuznetsova, Irina G.
AU - Belimov, Andrey A.
AU - Andronov, Evgeny E.
AU - Chirak, Elizaveta R.
AU - Popova, Janna P.
AU - Verkhozina, Alla V.
AU - Willems, Anne
AU - Tikhonovich, Igor A.
PY - 2018/5/1
Y1 - 2018/5/1
N2 - Gram-negative strains Tri-36, Tri-38, Tri-48T and Tri-53 were isolated from root nodules of the relict legume Oxytropis triphylla (Pall.) Pers. originating from Zunduk Cape (Baikal Lake region, Russia). 16S rRNA gene sequencing showed that the novel isolates were phylogenetically closest to the type strains Phyllobacterium sophorae LMG 27899T, Phyllobacterium brassicacearum LMG 22836T, Phyllobacterium endophyticum LMG 26470T and Phyllobacterium bourgognense LMG 22837T while similarity levels between the isolates and the most closely related strain P. endophyticum LMG 26470T were 98.8-99.5%. The recA and glnII genes of the isolates showed highest sequence similarities with P. sophorae LMG 27899T (95.4 and 89.5%, respectively) and P. brassicacearum LMG 22836T (91.4 and 85.1%, respectively). Comparative analysis of phenotypic properties between the novel isolates and the closest reference strains P. sophorae LMG 27899T, P. brassicacearum LMG 22836T and P. endophyticum LMG 26470T was performed using a microassay system. Average nucleotide identities between the whole genome sequences of the isolates Tri-38 and Tri-48T and P. sophorae LMG 27899T, P. brassicacearum LMG 22836T and P. endophyticum LMG 26470T ranged from 79.23% for P. endophyticum LMG 26470T to 85.74% for P. sophorae LMG 27899T. The common nodABC genes required for legume nodulation were absent from strains Tri-38 and Tri-48T, although some other symbiotic nod and fix genes were detected. On the basis of genotypic and phenotypic analysis, a novel species, Phyllobacterium zundukense sp. nov. (type strain Tri-48T=LMG 30371T=RCAM 03910T), is proposed.
AB - Gram-negative strains Tri-36, Tri-38, Tri-48T and Tri-53 were isolated from root nodules of the relict legume Oxytropis triphylla (Pall.) Pers. originating from Zunduk Cape (Baikal Lake region, Russia). 16S rRNA gene sequencing showed that the novel isolates were phylogenetically closest to the type strains Phyllobacterium sophorae LMG 27899T, Phyllobacterium brassicacearum LMG 22836T, Phyllobacterium endophyticum LMG 26470T and Phyllobacterium bourgognense LMG 22837T while similarity levels between the isolates and the most closely related strain P. endophyticum LMG 26470T were 98.8-99.5%. The recA and glnII genes of the isolates showed highest sequence similarities with P. sophorae LMG 27899T (95.4 and 89.5%, respectively) and P. brassicacearum LMG 22836T (91.4 and 85.1%, respectively). Comparative analysis of phenotypic properties between the novel isolates and the closest reference strains P. sophorae LMG 27899T, P. brassicacearum LMG 22836T and P. endophyticum LMG 26470T was performed using a microassay system. Average nucleotide identities between the whole genome sequences of the isolates Tri-38 and Tri-48T and P. sophorae LMG 27899T, P. brassicacearum LMG 22836T and P. endophyticum LMG 26470T ranged from 79.23% for P. endophyticum LMG 26470T to 85.74% for P. sophorae LMG 27899T. The common nodABC genes required for legume nodulation were absent from strains Tri-38 and Tri-48T, although some other symbiotic nod and fix genes were detected. On the basis of genotypic and phenotypic analysis, a novel species, Phyllobacterium zundukense sp. nov. (type strain Tri-48T=LMG 30371T=RCAM 03910T), is proposed.
KW - Oxytropis triphylla (Pall.) Pers
KW - Phyllobacterium zundukense
KW - Relict legumes
KW - Root nodule bacteria
UR - http://www.scopus.com/inward/record.url?scp=85046553057&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.002722
DO - 10.1099/ijsem.0.002722
M3 - Article
C2 - 29620492
AN - SCOPUS:85046553057
VL - 68
SP - 1644
EP - 1651
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 5
M1 - 002722
ER -
ID: 36778740