Pharmacological screening of a new alpha-2 adrenergic receptor agonist, mafedine, in zebrafish

Yuriy I. Sysoev, Darya A. Meshalkina, Daniil V. Petrov, Sergey V. Okovityi, Pavel E. Musienko, Allan V. Kalueff

Research output

Abstract

Pharmacological agents acting at alpha-2 adrenergic receptors are widely used in physiology and neuroscience research. Mounting evidence of their potential utility in clinical and experimental psychopharmacology, necessitates new models and novel model organisms for their screening. Here, we characterize behavioral effects of mafedine (6-oxo-1-phenyl-2- (phenylamino)-1,6-dihydropyrimidine-4-sodium olate), a novel drug with alpha-2 adrenergic receptor agonistic effects, in adult zebrafish (Danio rerio) in the novel tank test of anxiety and activity. Following an acute 20-min exposure, mafedine at 60 mg/L produced a mild psychostimulant action with some anxiogenic-like effects. Repeated acute 20-min/day administration of mafedine for 7 consecutive days at 1, 5 and 10 mg/L had a similar action on fish behavior as an acute exposure to 60 mg/L. Since mafedine demonstrated robust behavioral effects in zebrafish – a sensitive vertebrate aquatic model, it is likely that it may modulate rodent and human behavior as well. Thus, further studies are needed to explore this possibility in detail, and whether it may foster clinical application of mafedine and related alpha-2 adrenergic agents.

Original languageEnglish
Pages (from-to)234-239
Number of pages6
JournalNeuroscience Letters
Volume701
DOIs
Publication statusPublished - 14 May 2019

Fingerprint

Adrenergic alpha-2 Receptor Agonists
Zebrafish
Adrenergic alpha-2 Receptors
Pharmacology
Psychopharmacology
Neurosciences
Adrenergic Agents
Vertebrates
Rodentia
Fishes
Anxiety
Sodium
Research
Pharmaceutical Preparations

Scopus subject areas

  • Neuroscience(all)

Cite this

Sysoev, Yuriy I. ; Meshalkina, Darya A. ; Petrov, Daniil V. ; Okovityi, Sergey V. ; Musienko, Pavel E. ; Kalueff, Allan V. / Pharmacological screening of a new alpha-2 adrenergic receptor agonist, mafedine, in zebrafish. In: Neuroscience Letters. 2019 ; Vol. 701. pp. 234-239.
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Pharmacological screening of a new alpha-2 adrenergic receptor agonist, mafedine, in zebrafish. / Sysoev, Yuriy I.; Meshalkina, Darya A.; Petrov, Daniil V.; Okovityi, Sergey V.; Musienko, Pavel E.; Kalueff, Allan V.

In: Neuroscience Letters, Vol. 701, 14.05.2019, p. 234-239.

Research output

TY - JOUR

T1 - Pharmacological screening of a new alpha-2 adrenergic receptor agonist, mafedine, in zebrafish

AU - Sysoev, Yuriy I.

AU - Meshalkina, Darya A.

AU - Petrov, Daniil V.

AU - Okovityi, Sergey V.

AU - Musienko, Pavel E.

AU - Kalueff, Allan V.

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N2 - Pharmacological agents acting at alpha-2 adrenergic receptors are widely used in physiology and neuroscience research. Mounting evidence of their potential utility in clinical and experimental psychopharmacology, necessitates new models and novel model organisms for their screening. Here, we characterize behavioral effects of mafedine (6-oxo-1-phenyl-2- (phenylamino)-1,6-dihydropyrimidine-4-sodium olate), a novel drug with alpha-2 adrenergic receptor agonistic effects, in adult zebrafish (Danio rerio) in the novel tank test of anxiety and activity. Following an acute 20-min exposure, mafedine at 60 mg/L produced a mild psychostimulant action with some anxiogenic-like effects. Repeated acute 20-min/day administration of mafedine for 7 consecutive days at 1, 5 and 10 mg/L had a similar action on fish behavior as an acute exposure to 60 mg/L. Since mafedine demonstrated robust behavioral effects in zebrafish – a sensitive vertebrate aquatic model, it is likely that it may modulate rodent and human behavior as well. Thus, further studies are needed to explore this possibility in detail, and whether it may foster clinical application of mafedine and related alpha-2 adrenergic agents.

AB - Pharmacological agents acting at alpha-2 adrenergic receptors are widely used in physiology and neuroscience research. Mounting evidence of their potential utility in clinical and experimental psychopharmacology, necessitates new models and novel model organisms for their screening. Here, we characterize behavioral effects of mafedine (6-oxo-1-phenyl-2- (phenylamino)-1,6-dihydropyrimidine-4-sodium olate), a novel drug with alpha-2 adrenergic receptor agonistic effects, in adult zebrafish (Danio rerio) in the novel tank test of anxiety and activity. Following an acute 20-min exposure, mafedine at 60 mg/L produced a mild psychostimulant action with some anxiogenic-like effects. Repeated acute 20-min/day administration of mafedine for 7 consecutive days at 1, 5 and 10 mg/L had a similar action on fish behavior as an acute exposure to 60 mg/L. Since mafedine demonstrated robust behavioral effects in zebrafish – a sensitive vertebrate aquatic model, it is likely that it may modulate rodent and human behavior as well. Thus, further studies are needed to explore this possibility in detail, and whether it may foster clinical application of mafedine and related alpha-2 adrenergic agents.

KW - Alpha-2 agonists

KW - Anxiogenic effects

KW - Mafedine

KW - Novel tank test

KW - Psychostimulant action

KW - Zebrafish

KW - Dose-Response Relationship, Drug

KW - Adrenergic alpha-2 Receptor Agonists/pharmacology

KW - Animals

KW - Behavior, Animal/drug effects

KW - Drug Evaluation, Preclinical/methods

KW - Mafenide/pharmacology

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DO - 10.1016/j.neulet.2019.03.001

M3 - Article

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AN - SCOPUS:85062723875

VL - 701

SP - 234

EP - 239

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

ER -