Peculiarities of Cyto- and Chemoarchitectonics of Human Entorhinal Cortex during the Fetal Period

P A Zykin, I A Moiseenko, L A Tkachenko, R A Nasyrov, E A Tsvetkov, E I Krasnoshchekova

Research output

Abstract

We studied peculiarities of the structure of human entorhinal cortex at weeks 20-26 of gestation (10 hemispheres). The samples were Nissl-stained and immunohistochemically treated with antibodies to parvalbumin, calretinin, calbindin, and cytoskeleton proteins (MAP2 and N200). 3D-reconstruction of the entorhinal cortex from serial sections was performed, caudomedial and rostrolateral areas were isolated. Parvalbumin+cells in layer I, discrete distribution of layer II cells with colocalization of MAP2 and calretinin at the border with layer I, and two sublayers Va and Vb with MAP2+neurons were typical for the caudomedial area. Rostrolateral area was characterized by the homogenous layer II with big amount of cells, high density of MAP2+neurons only in layer III, and the unique layer V. Reelin+Cajal-Retzius cells and N200+fiber plexus in layer I were observed in the caudomedial and rostrolateral areas of the entorhial cortex. Layer IV was represented by a cell-free desiccant.

Original languageEnglish
Pages (from-to)497-501
Number of pages5
JournalBulletin of Experimental Biology and Medicine
Volume164
Issue number4
Early online date2018
DOIs
Publication statusPublished - Mar 2018

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Calbindin 2
Parvalbumins
Entorhinal Cortex
Neurons
Hygroscopic Agents
Calbindins
Antibodies
Fibers
Cytoskeleton
Proteins
Cell Count
Pregnancy

Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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title = "Peculiarities of Cyto- and Chemoarchitectonics of Human Entorhinal Cortex during the Fetal Period",
abstract = "We studied peculiarities of the structure of human entorhinal cortex at weeks 20-26 of gestation (10 hemispheres). The samples were Nissl-stained and immunohistochemically treated with antibodies to parvalbumin, calretinin, calbindin, and cytoskeleton proteins (MAP2 and N200). 3D-reconstruction of the entorhinal cortex from serial sections was performed, caudomedial and rostrolateral areas were isolated. Parvalbumin+cells in layer I, discrete distribution of layer II cells with colocalization of MAP2 and calretinin at the border with layer I, and two sublayers Va and Vb with MAP2+neurons were typical for the caudomedial area. Rostrolateral area was characterized by the homogenous layer II with big amount of cells, high density of MAP2+neurons only in layer III, and the unique layer V. Reelin+Cajal-Retzius cells and N200+fiber plexus in layer I were observed in the caudomedial and rostrolateral areas of the entorhial cortex. Layer IV was represented by a cell-free desiccant.",
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AU - Moiseenko, I A

AU - Tkachenko, L A

AU - Nasyrov, R A

AU - Tsvetkov, E A

AU - Krasnoshchekova, E I

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