Optimization of Metabolite Profiling for Black Medick (Medicago lupulina) and Peas (Pisum sativum)

R. K. Puzanskiy, V. V. Yemelyanov, M. S. Kliukova, A. L. Shavarda, O. Yu Shtark, A. P. Yurkov, M. F. Shishova

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Metabolic profiling is a key approach in current basic and applied research in biology. Comparative analysis of different metabolite extraction methods for pea (P. sativum) and black medick (M. lupulina) made it possible to find the optimal conditions for metabolite extraction and subsequent detection by gas chromatography coupled with mass spectrometry. The optimized method was shown to be reliable for assessment of the organ and species metabolic profiles for roots and leaves in pea and black medick plants.

Original languageEnglish
Pages (from-to)442-448
Number of pages7
JournalApplied Biochemistry and Microbiology
Volume54
Issue number4
DOIs
StatePublished - 13 Jul 2018

Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

Keywords

  • black medick
  • extraction
  • gas chromatography
  • mass spectrometry
  • metabolite
  • peas

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