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On the Polymer System “PEG–Dextran” for Isolating Nanoscale Extracellular Vesicles. / Слюсаренко, Мария Александровна; Евлампиева, Наталья Петровна; Везо, Ольга Сергеевна; Malek, Anastasia.

In: Nanobiotechnology Reports, Vol. 18, No. 6, 01.12.2023, p. 842–853.

Research output: Contribution to journal › Article › peer-review

Harvard

Слюсаренко, МА, Евлампиева, НП, Везо, ОС & Malek, A 2023, 'On the Polymer System “PEG–Dextran” for Isolating Nanoscale Extracellular Vesicles', Nanobiotechnology Reports, vol. 18, no. 6, pp. 842–853. https://doi.org/10.1134/s2635167623601043

APA

Слюсаренко, М. А., Евлампиева, Н. П., Везо, О. С., & Malek, A. (2023). On the Polymer System “PEG–Dextran” for Isolating Nanoscale Extracellular Vesicles. Nanobiotechnology Reports, 18(6), 842–853. https://doi.org/10.1134/s2635167623601043

Vancouver

Слюсаренко МА, Евлампиева НП, Везо ОС, Malek A. On the Polymer System “PEG–Dextran” for Isolating Nanoscale Extracellular Vesicles. Nanobiotechnology Reports. 2023 Dec 1;18(6):842–853. https://doi.org/10.1134/s2635167623601043

Author

Слюсаренко, Мария Александровна ; Евлампиева, Наталья Петровна ; Везо, Ольга Сергеевна ; Malek, Anastasia. / On the Polymer System “PEG–Dextran” for Isolating Nanoscale Extracellular Vesicles. In: Nanobiotechnology Reports. 2023 ; Vol. 18, No. 6. pp. 842–853.

BibTeX

@article{f02be889f64e4f62825272f493c0b160,
title = "On the Polymer System “PEG–Dextran” for Isolating Nanoscale Extracellular Vesicles",
abstract = "Abstract: It is shown that the two-phase polymer system “polyethylene glycol–dextran” can be used with high efficiency for isolating nanoscale vesicles from human blood plasma, and a corresponding methodology is developed. The optimal parameters of the system for this purpose are determined: molecular weight of polymers, their weight ratio, operating temperature. The mechanism of interaction between the components of the polymer system and plasma components is also studied. It is established that the release of vesicles in a given size range is due to the choice of the composition of the polymer system in the region below its binodal and compliance with a certain quantitative ratio of the hydrodynamic volumes of dextran and polyethylene-glycol molecules in the plasma. The proposed method is an inexpensive and convenient way to concentrate vesicles for their subsequent biochemical analysis.",
author = "Слюсаренко, {Мария Александровна} and Евлампиева, {Наталья Петровна} and Везо, {Ольга Сергеевна} and Anastasia Malek",
year = "2023",
month = dec,
day = "1",
doi = "10.1134/s2635167623601043",
language = "English",
volume = "18",
pages = "842–853",
journal = "Nanobiotechnology Reports",
issn = "2635-1676",
publisher = "Pleiades Publishing",
number = "6",

}

RIS

TY - JOUR

T1 - On the Polymer System “PEG–Dextran” for Isolating Nanoscale Extracellular Vesicles

AU - Слюсаренко, Мария Александровна

AU - Евлампиева, Наталья Петровна

AU - Везо, Ольга Сергеевна

AU - Malek, Anastasia

PY - 2023/12/1

Y1 - 2023/12/1

N2 - Abstract: It is shown that the two-phase polymer system “polyethylene glycol–dextran” can be used with high efficiency for isolating nanoscale vesicles from human blood plasma, and a corresponding methodology is developed. The optimal parameters of the system for this purpose are determined: molecular weight of polymers, their weight ratio, operating temperature. The mechanism of interaction between the components of the polymer system and plasma components is also studied. It is established that the release of vesicles in a given size range is due to the choice of the composition of the polymer system in the region below its binodal and compliance with a certain quantitative ratio of the hydrodynamic volumes of dextran and polyethylene-glycol molecules in the plasma. The proposed method is an inexpensive and convenient way to concentrate vesicles for their subsequent biochemical analysis.

AB - Abstract: It is shown that the two-phase polymer system “polyethylene glycol–dextran” can be used with high efficiency for isolating nanoscale vesicles from human blood plasma, and a corresponding methodology is developed. The optimal parameters of the system for this purpose are determined: molecular weight of polymers, their weight ratio, operating temperature. The mechanism of interaction between the components of the polymer system and plasma components is also studied. It is established that the release of vesicles in a given size range is due to the choice of the composition of the polymer system in the region below its binodal and compliance with a certain quantitative ratio of the hydrodynamic volumes of dextran and polyethylene-glycol molecules in the plasma. The proposed method is an inexpensive and convenient way to concentrate vesicles for their subsequent biochemical analysis.

UR - https://www.mendeley.com/catalogue/472a6dfd-5fd6-307c-a70e-bf9007eeb0bc/

U2 - 10.1134/s2635167623601043

DO - 10.1134/s2635167623601043

M3 - Article

VL - 18

SP - 842

EP - 853

JO - Nanobiotechnology Reports

JF - Nanobiotechnology Reports

SN - 2635-1676

IS - 6

ER -

ID: 126516521