New supramolecular AuI-CuI complex as potential luminescent label for proteins

D.V. Krupenya, P.A. Snegurov, E.V. Grachova, V.V. Gurzhiy, S.P. Tunik, A.S. Melnikov, P.Y. Serdobintsev, E.G. Vlakh, E.S. Sinitsyna, T.B. Tennikova

Research output

11 Citations (Scopus)

Abstract

A novel supramolecular [Au6Cu2(C2C6H4-4-COONC4H4O2)6(Ph2PC6H4PPh2)3](PF6)2 complex functionalized with succinimide alkynyl substituent has been synthesized and characterized using X-ray crystallography, mass spectrometry and NMR spectroscopy. Like the other complexes of this class demonstrates bright emission in acetone and dichloromethane solutions with the excited state lifetime in microsecond domain. This complex readily reacts with surface amine group of proteins/enzymes (human serum albumin, rabbit anti-HSA antibodies, α-chymotrypsin) to give covalent conjugates, which contain up to five molecules of the luminescent label bound to the biomolecule. The conjugates keep high level of the phosphorescent label emission but in contrast to the parent complex molecule display excellent solubility and high stability in physiological media. Investigation of biological activity of the conjugates also showed that specific structure of the biomolecules remains nearly unchanged upon bonding with the label that is indi
Original languageEnglish
Pages (from-to)12521-12528
JournalInorganic Chemistry
Volume52
Issue number21
DOIs
Publication statusPublished - 2013

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Labels
Biomolecules
proteins
succinimides
Proteins
Molecules
Methylene Chloride
X ray crystallography
rabbits
Chymotrypsin
Acetone
activity (biology)
Bioactivity
antibodies
albumins
Excited states
Serum Albumin
serums
acetone
Nuclear magnetic resonance spectroscopy

Cite this

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title = "New supramolecular AuI-CuI complex as potential luminescent label for proteins",
abstract = "A novel supramolecular [Au6Cu2(C2C6H4-4-COONC4H4O2)6(Ph2PC6H4PPh2)3](PF6)2 complex functionalized with succinimide alkynyl substituent has been synthesized and characterized using X-ray crystallography, mass spectrometry and NMR spectroscopy. Like the other complexes of this class demonstrates bright emission in acetone and dichloromethane solutions with the excited state lifetime in microsecond domain. This complex readily reacts with surface amine group of proteins/enzymes (human serum albumin, rabbit anti-HSA antibodies, α-chymotrypsin) to give covalent conjugates, which contain up to five molecules of the luminescent label bound to the biomolecule. The conjugates keep high level of the phosphorescent label emission but in contrast to the parent complex molecule display excellent solubility and high stability in physiological media. Investigation of biological activity of the conjugates also showed that specific structure of the biomolecules remains nearly unchanged upon bonding with the label that is indi",
keywords = "Phosphorescent label, Proteins, Bioconjugates, Supramolecular complexes, Gold",
author = "D.V. Krupenya and P.A. Snegurov and E.V. Grachova and V.V. Gurzhiy and S.P. Tunik and A.S. Melnikov and P.Y. Serdobintsev and E.G. Vlakh and E.S. Sinitsyna and T.B. Tennikova",
year = "2013",
doi = "10.1021/ic401569n",
language = "English",
volume = "52",
pages = "12521--12528",
journal = "Inorganic Chemistry",
issn = "0020-1669",
publisher = "American Chemical Society",
number = "21",

}

New supramolecular AuI-CuI complex as potential luminescent label for proteins. / Krupenya, D.V.; Snegurov, P.A.; Grachova, E.V.; Gurzhiy, V.V.; Tunik, S.P.; Melnikov, A.S.; Serdobintsev, P.Y.; Vlakh, E.G.; Sinitsyna, E.S.; Tennikova, T.B.

In: Inorganic Chemistry, Vol. 52, No. 21, 2013, p. 12521-12528.

Research output

TY - JOUR

T1 - New supramolecular AuI-CuI complex as potential luminescent label for proteins

AU - Krupenya, D.V.

AU - Snegurov, P.A.

AU - Grachova, E.V.

AU - Gurzhiy, V.V.

AU - Tunik, S.P.

AU - Melnikov, A.S.

AU - Serdobintsev, P.Y.

AU - Vlakh, E.G.

AU - Sinitsyna, E.S.

AU - Tennikova, T.B.

PY - 2013

Y1 - 2013

N2 - A novel supramolecular [Au6Cu2(C2C6H4-4-COONC4H4O2)6(Ph2PC6H4PPh2)3](PF6)2 complex functionalized with succinimide alkynyl substituent has been synthesized and characterized using X-ray crystallography, mass spectrometry and NMR spectroscopy. Like the other complexes of this class demonstrates bright emission in acetone and dichloromethane solutions with the excited state lifetime in microsecond domain. This complex readily reacts with surface amine group of proteins/enzymes (human serum albumin, rabbit anti-HSA antibodies, α-chymotrypsin) to give covalent conjugates, which contain up to five molecules of the luminescent label bound to the biomolecule. The conjugates keep high level of the phosphorescent label emission but in contrast to the parent complex molecule display excellent solubility and high stability in physiological media. Investigation of biological activity of the conjugates also showed that specific structure of the biomolecules remains nearly unchanged upon bonding with the label that is indi

AB - A novel supramolecular [Au6Cu2(C2C6H4-4-COONC4H4O2)6(Ph2PC6H4PPh2)3](PF6)2 complex functionalized with succinimide alkynyl substituent has been synthesized and characterized using X-ray crystallography, mass spectrometry and NMR spectroscopy. Like the other complexes of this class demonstrates bright emission in acetone and dichloromethane solutions with the excited state lifetime in microsecond domain. This complex readily reacts with surface amine group of proteins/enzymes (human serum albumin, rabbit anti-HSA antibodies, α-chymotrypsin) to give covalent conjugates, which contain up to five molecules of the luminescent label bound to the biomolecule. The conjugates keep high level of the phosphorescent label emission but in contrast to the parent complex molecule display excellent solubility and high stability in physiological media. Investigation of biological activity of the conjugates also showed that specific structure of the biomolecules remains nearly unchanged upon bonding with the label that is indi

KW - Phosphorescent label

KW - Proteins

KW - Bioconjugates

KW - Supramolecular complexes

KW - Gold

U2 - 10.1021/ic401569n

DO - 10.1021/ic401569n

M3 - Article

VL - 52

SP - 12521

EP - 12528

JO - Inorganic Chemistry

JF - Inorganic Chemistry

SN - 0020-1669

IS - 21

ER -