New data on the fine structure of Deuteramoeba mycophaga CCAP 1586/1 (Amoebozoa, Tubulinea)

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New data on the fine structure of Deuteramoeba mycophaga CCAP 1586/1 (Amoebozoa, Tubulinea). / Камышацкая, Оксана Геннадьевна ; Насонова, Елена Станиславовна ; Бондаренко, Наталья Ивановна ; Смирнов, Алексей Валерьевич.

In: European Journal of Protistology, Vol. 82, 125853, 02.2022.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{0d369bc704ce406d85bdec6811e10733,

title = "New data on the fine structure of Deuteramoeba mycophaga CCAP 1586/1 (Amoebozoa, Tubulinea)",

abstract = "The genus Deuteramoeba is one of the six amoebae genera belonging to the best-known amoeba family – Amoebidae (Amoebozoa, Tubulinea), containing such a popular species as Amoeba proteus. However, members of other genera of the family Amoebidae are much less known, and most of the studies of their morphology and ultrastructure date back to the 1970s and 1980s. Since these “classical” species are believed to be “well studied”, their morphology and fine structure rarely become a subject of re-investigation. The absence of modern morphological data may be critical when molecular data of the type strain are not available, and the only way to identify a species is by morphological comparison. For this paper, we performed an ultrastructural study of the strain CCAP 1586/1 – the type strain of the species Deuteramoeba mycophaga. Our study revealed new details of the nuclear structure, including a peripheral layer of filaments and a heterogeneous nucleolus, and provided new data on the cytoplasmic inclusions of this species. We performed a whole-genome amplification of the DNA from a single amoeba cell followed by NGS sequencing and searched for genetic evidence for the presence of a putative nuclear parasite detected in 2017, but found no evidence for the presence of Opisthosporidia.",

keywords = "Amoebidae, Amoebozoa, Deuteramoeba, Ultrastructure",

author = "Камышацкая, {Оксана Геннадьевна} and Насонова, {Елена Станиславовна} and Бондаренко, {Наталья Ивановна} and Смирнов, {Алексей Валерьевич}",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier GmbH",

year = "2022",

month = feb,

doi = "10.1016/j.ejop.2021.125853",

language = "English",

volume = "82",

journal = "European Journal of Protistology",

issn = "0932-4739",

publisher = "Urban und Fischer Verlag GmbH und Co. KG",

}

RIS

TY - JOUR

T1 - New data on the fine structure of Deuteramoeba mycophaga CCAP 1586/1 (Amoebozoa, Tubulinea)

AU - Камышацкая, Оксана Геннадьевна

AU - Насонова, Елена Станиславовна

AU - Бондаренко, Наталья Ивановна

AU - Смирнов, Алексей Валерьевич

PY - 2022/2

Y1 - 2022/2

N2 - The genus Deuteramoeba is one of the six amoebae genera belonging to the best-known amoeba family – Amoebidae (Amoebozoa, Tubulinea), containing such a popular species as Amoeba proteus. However, members of other genera of the family Amoebidae are much less known, and most of the studies of their morphology and ultrastructure date back to the 1970s and 1980s. Since these “classical” species are believed to be “well studied”, their morphology and fine structure rarely become a subject of re-investigation. The absence of modern morphological data may be critical when molecular data of the type strain are not available, and the only way to identify a species is by morphological comparison. For this paper, we performed an ultrastructural study of the strain CCAP 1586/1 – the type strain of the species Deuteramoeba mycophaga. Our study revealed new details of the nuclear structure, including a peripheral layer of filaments and a heterogeneous nucleolus, and provided new data on the cytoplasmic inclusions of this species. We performed a whole-genome amplification of the DNA from a single amoeba cell followed by NGS sequencing and searched for genetic evidence for the presence of a putative nuclear parasite detected in 2017, but found no evidence for the presence of Opisthosporidia.

AB - The genus Deuteramoeba is one of the six amoebae genera belonging to the best-known amoeba family – Amoebidae (Amoebozoa, Tubulinea), containing such a popular species as Amoeba proteus. However, members of other genera of the family Amoebidae are much less known, and most of the studies of their morphology and ultrastructure date back to the 1970s and 1980s. Since these “classical” species are believed to be “well studied”, their morphology and fine structure rarely become a subject of re-investigation. The absence of modern morphological data may be critical when molecular data of the type strain are not available, and the only way to identify a species is by morphological comparison. For this paper, we performed an ultrastructural study of the strain CCAP 1586/1 – the type strain of the species Deuteramoeba mycophaga. Our study revealed new details of the nuclear structure, including a peripheral layer of filaments and a heterogeneous nucleolus, and provided new data on the cytoplasmic inclusions of this species. We performed a whole-genome amplification of the DNA from a single amoeba cell followed by NGS sequencing and searched for genetic evidence for the presence of a putative nuclear parasite detected in 2017, but found no evidence for the presence of Opisthosporidia.

KW - Amoebidae

KW - Amoebozoa

KW - Deuteramoeba

KW - Ultrastructure

UR - https://www.mendeley.com/catalogue/0b7ee12d-7156-31bb-867f-f91a5e40cdad/

UR - http://www.scopus.com/inward/record.url?scp=85122511156&partnerID=8YFLogxK

U2 - 10.1016/j.ejop.2021.125853

DO - 10.1016/j.ejop.2021.125853

M3 - Article

VL - 82

JO - European Journal of Protistology

JF - European Journal of Protistology

SN - 0932-4739

M1 - 125853

ER -

ID: 85979304