Molecular Phylogeny of Polychaos annulatum (Amoebozoa, Tubulinea, Euamoebida) Shows that Genus Polychaos Belongs to the Family Hartmannellidae

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Molecular Phylogeny of Polychaos annulatum (Amoebozoa, Tubulinea, Euamoebida) Shows that Genus Polychaos Belongs to the Family Hartmannellidae. / Kamyshatskaya, Oksana G. ; Bondarenko, Natalya I. ; Mesentsev, Yelisei S. ; Chistyakova, Ludmila V. ; Nassonova, Elena S.; Smirnov, Alexey V. .

In: Journal of Eukaryotic Microbiology, Vol. 67, No. 3, 01.05.2020, p. 321-326.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{cea61f1f45dc443f9f9e66119d125706,

title = "Molecular Phylogeny of Polychaos annulatum (Amoebozoa, Tubulinea, Euamoebida) Shows that Genus Polychaos Belongs to the Family Hartmannellidae",

abstract = "We have obtained a sequence of the 18S rRNA gene of the species Polychaos annulatum (Penard 1902) Smirnov et Goodkov 1998 using the isolation of a single nucleus from an amoeba cell. Attempts to amplify the 18S rRNA gene from the DNA of this species by conventional PCR were not successful, so we applied the whole genome amplification of the nuclear DNA followed by NGS‐sequencing. The 18S rRNA gene was found among the resulting contigs. The analysis unexpectedly shows that Polychaos annulatum robustly groups within the family Hartmannellidae, but not Amoebidae. This finding warrants revision of the basic morphological criteria used to classify Euamoebida families and show that “proteus‐type” amoebae may belong to other families rather than Amoebidae. This makes taxonomic assignments of such species more complex and the borders between Euamoebida families more nuanced. It is getting evident that molecular data are necessary to clarify the position of species even in this most “classical” order of naked lobose amoebae.",

keywords = "amoeba, Amoebidae, Systematics, taxonomy, systematics, Amoeba, FINE-STRUCTURE, ALGORITHM, CLASSIFICATION, PENARD, LOBOSEA, POSITION, LIMAX AMEBAS, GYMNAMOEBIA",

author = "Kamyshatskaya, {Oksana G.} and Bondarenko, {Natalya I.} and Mesentsev, {Yelisei S.} and Chistyakova, {Ludmila V.} and Nassonova, {Elena S.} and Smirnov, {Alexey V.}",

note = "Publisher Copyright: {\textcopyright} 2020 International Society of Protistologists",

year = "2020",

month = may,

day = "1",

doi = "10.1111/jeu.12782",

language = "English",

volume = "67",

pages = "321--326",

journal = "Journal of Eukaryotic Microbiology",

issn = "1066-5234",

publisher = "Wiley-Blackwell",

number = "3",

}

RIS

TY - JOUR

T1 - Molecular Phylogeny of Polychaos annulatum (Amoebozoa, Tubulinea, Euamoebida) Shows that Genus Polychaos Belongs to the Family Hartmannellidae

AU - Kamyshatskaya, Oksana G.

AU - Bondarenko, Natalya I.

AU - Mesentsev, Yelisei S.

AU - Chistyakova, Ludmila V.

AU - Nassonova, Elena S.

AU - Smirnov, Alexey V.

PY - 2020/5/1

Y1 - 2020/5/1

N2 - We have obtained a sequence of the 18S rRNA gene of the species Polychaos annulatum (Penard 1902) Smirnov et Goodkov 1998 using the isolation of a single nucleus from an amoeba cell. Attempts to amplify the 18S rRNA gene from the DNA of this species by conventional PCR were not successful, so we applied the whole genome amplification of the nuclear DNA followed by NGS‐sequencing. The 18S rRNA gene was found among the resulting contigs. The analysis unexpectedly shows that Polychaos annulatum robustly groups within the family Hartmannellidae, but not Amoebidae. This finding warrants revision of the basic morphological criteria used to classify Euamoebida families and show that “proteus‐type” amoebae may belong to other families rather than Amoebidae. This makes taxonomic assignments of such species more complex and the borders between Euamoebida families more nuanced. It is getting evident that molecular data are necessary to clarify the position of species even in this most “classical” order of naked lobose amoebae.

AB - We have obtained a sequence of the 18S rRNA gene of the species Polychaos annulatum (Penard 1902) Smirnov et Goodkov 1998 using the isolation of a single nucleus from an amoeba cell. Attempts to amplify the 18S rRNA gene from the DNA of this species by conventional PCR were not successful, so we applied the whole genome amplification of the nuclear DNA followed by NGS‐sequencing. The 18S rRNA gene was found among the resulting contigs. The analysis unexpectedly shows that Polychaos annulatum robustly groups within the family Hartmannellidae, but not Amoebidae. This finding warrants revision of the basic morphological criteria used to classify Euamoebida families and show that “proteus‐type” amoebae may belong to other families rather than Amoebidae. This makes taxonomic assignments of such species more complex and the borders between Euamoebida families more nuanced. It is getting evident that molecular data are necessary to clarify the position of species even in this most “classical” order of naked lobose amoebae.

KW - amoeba

KW - Amoebidae

KW - Systematics

KW - taxonomy

KW - systematics

KW - Amoeba

KW - FINE-STRUCTURE

KW - ALGORITHM

KW - CLASSIFICATION

KW - PENARD

KW - LOBOSEA

KW - POSITION

KW - LIMAX AMEBAS

KW - GYMNAMOEBIA

UR - http://www.scopus.com/inward/record.url?scp=85078656624&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/1bb82c51-5bb2-3fe6-9425-a9f857dbaaf3/

U2 - 10.1111/jeu.12782

DO - 10.1111/jeu.12782

M3 - Article

VL - 67

SP - 321

EP - 326

JO - Journal of Eukaryotic Microbiology

JF - Journal of Eukaryotic Microbiology

SN - 1066-5234

IS - 3

ER -

ID: 49048106