Molecular oxygen mapping in biological samples by time-correlated single photon counting technique and Ir(III)-based complexes

Marina V. Shirmanova, Vladislav I. Shcheslavskiy, Maria M. Lukina, Varvara V. Dudenkova, Ilya S. Kritchenkov, Anastasia I. Solomatina, Sergey P. Tunik

Research outputpeer-review

Abstract

Non-invasive optical techniques on the basis of quenching of phosphorescence by molecular oxygen represent a powerful tool to perform mapping of oxygen content in biological systems. The development of phosphorescent oxygensensitive probes suitable for biological applications, especially in vivo, remains a challenging task. In this paper we applied for biological investigations one novel and one previously published Ir(III)-based complexes. This complexes demonstrate low cytotoxicity at the concentrations ≤70 μM and good uptake by cultured cancer cells. Both compounds were primarily located within the cytoplasm. Using TCSPC-based phosphorescence lifetime macro-imaging in vivo, we found that IR-2 complex shows detectable phosphorescence in tumor tissue in mice upon local injection. These results illustrate the high potential of organometallic complexes under study for mapping of oxygen level in cells and tissues.

Original languageEnglish
Title of host publicationOptical Biopsy XVIII
Subtitle of host publicationToward Real-Time Spectroscopic Imaging and Diagnosis
EditorsRobert R. Alfano, Stavros G. Demos, Angela B. Seddon
PublisherSPIE
ISBN (Electronic)9781510632318
DOIs
Publication statusPublished - 1 Jan 2020
EventOptical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis 2020 - San Francisco
Duration: 2 Feb 20205 Feb 2020

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11234
ISSN (Print)1605-7422

Conference

ConferenceOptical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis 2020
CountryUnited States
CitySan Francisco
Period2/02/205/02/20

Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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