Molecular mechanisms of adaptation to the habitat depth in visual pigments of A. subulata and L. forbesi squids: On the role of the S270F substitution

Dmitrii M. Nikolaev, Demid E. Osipov, Daniil M. Strashkov, Sergey Yu. Vyazmin, Vasily E. Akulov, Denis V. Kravtcov, Oleg B. Chakchir, Maxim S. Panov, Mikhail N. Ryazantsev

Research output: Contribution to journalReview articlepeer-review

3 Scopus citations

Abstract

Revealing the mechanisms of animal adaptation to different habitats is one of the central tasks of evolutionary physiology. A particular case of such adaptation is the visual adaptation of marine species to different depth ranges. Because water absorbs more intensively longer wavelengths than shorter wavelengths, the increase of habitat depth shifts the visual perception of marine species towards tire blue region. In This study, we investigated the molecular mechanisms of such visual adaptation for two squid species - Alloteuthis subulata and Loligo forbesi. These species live at different depths (200 m and 300 m, respectively) and the absorption maximum of A. subulata visual rhodopsin is slightly red-shifted compared to L forbesi rhodopsin (499 and 494 nm, respectively). Previously, the amino acid sequences of these two species were found to differ in 22 sites with only seven of them being non-neutral substitutions, and the S270F substitution was proposed as a possible candidate responsible for the spectral shift. In this study, we constructed computational models of visual rhodopsins of these two squid species and determined the main factors that cause the 5 nm spectral shift between the two proteins. We find that the origin of this spectral shift is a consequence of a complex reorganization of the protein caused by at least two mutations including S270F. Moreover, the direct electrostatic effect of polar hydroxyl-bearing serine that replaces non polar phenylalanine is negligible due to the relatively long distance to the chromophore.

Original languageEnglish
Pages (from-to)44-50
JournalJournal of Integrated OMICS
Volume9
Issue number1
DOIs
StatePublished - 1 Jun 2019

Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Keywords

  • A. subulata vision
  • L. forbesi vision
  • molecular mechanisms of visual adaptation
  • non-direct tuning in rhodopsins
  • spectral tuning in rhodopsins
  • squid rhodopsins
  • Visual adaptation

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